本文選題：糞便腐熟 + 耐熱��； 參考：《黑龍江畜牧獸醫(yī)》2017年16期

【摘要】：為了提高畜禽糞便腐熟的效率,試驗(yàn)采用平板擴(kuò)散法和Folin-酚試劑比色法篩選耐熱產(chǎn)蛋白酶芽孢桿菌菌株,并對其進(jìn)行種屬鑒定和產(chǎn)芽孢條件優(yōu)化。結(jié)果表明:得到一株耐熱產(chǎn)蛋白酶活性較高的芽孢桿菌菌株N62,初步鑒定為甲基營養(yǎng)型芽孢桿菌(Bacillus methylotrophicus);最適的培養(yǎng)基組成為玉米粉1.0%,黃豆餅粉1.0%,MgSO_4·7H_2O 0.02%,FeSO_4·5H_2O 0.02%;最適的培養(yǎng)條件為初始pH值7,裝液量50 mL/250 mL,接種量8 mL,發(fā)酵時(shí)間72 h;優(yōu)化后芽孢產(chǎn)率為95.0%。
[Abstract]:In order to improve the efficiency of fecal decay of livestock and poultry, the strain of Bacillus thermophilus was screened by plate diffusion method and Folin-phenol reagent colorimetry, and its species identification and spore production conditions were optimized. The results showed that a strain of Bacillus methylotrophicusum with high protease activity was obtained and identified as Bacillus methylotrophicus.The optimum culture medium was corn powder 1.0, soybean cake 1.0 7H_2O 0.02 and FeSO4 5H_2O 0.02; the optimum culture conditions were obtained. The initial pH value was 7, the volume of solution was 50 mL/250 mL, the inoculation amount was 8 mL, the fermentation time was 72 h, and the yield of spore was 95.0%.
【作者單位】： 河北農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;河北眾邦生物技術(shù)有限公司;
【基金】：保定市科學(xué)研究與發(fā)展計(jì)劃項(xiàng)目(15ZN017)
【分類號】：S141.4
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本文編號：1823578