本文選題：蘭州百合 + 有機(jī)堆肥　； 參考：《西北農(nóng)業(yè)學(xué)報(bào)》2017年03期

【摘要】：以蘭州百合為試材,基于llumina MiSeq測(cè)序平臺(tái),探討施用堆肥與化肥對(duì)蘭州百合鱗莖鮮質(zhì)量及根際土壤細(xì)菌和真菌微生物群落的影響。結(jié)果表明,有機(jī)堆肥對(duì)增加百合鱗莖鮮質(zhì)量作用顯著,相比化肥增產(chǎn)76.6%。物種注釋結(jié)果顯示,細(xì)菌的門(mén)、綱、目、科、屬的數(shù)量在施用堆肥的土壤中都大于施用化肥的土壤,而經(jīng)施用化肥處理的土壤中的真菌各個(gè)分類(lèi)水平的數(shù)量大于施用有機(jī)肥的。從豐富度指數(shù)(Chao1)和多樣性指數(shù)(Shannon)分析,細(xì)菌群落整體變化小于真菌群落的變化。施用有機(jī)肥或化肥的樣品,一些細(xì)菌屬的相對(duì)豐度變化不明顯,這些細(xì)菌屬包含的大多數(shù)菌是有益菌。此外,在有機(jī)堆肥組的樣品中出現(xiàn)一些有益特有菌屬,例如野野村菌屬(Nonomuraea),而化肥組的樣品中發(fā)現(xiàn)致病特有菌屬(Aquicella)。在真菌屬中,鐮刀菌屬(Fusarium)、曲霉屬(Aspergillus)、青霉菌屬(Penicillium)和支頂孢屬(Acremonium)的豐度在有機(jī)肥處理下顯著降低,這些屬分布有很多潛在或機(jī)會(huì)致病真菌,這在一定程度上說(shuō)明施用有機(jī)肥能有效改善百合根際土壤微生物群落。
[Abstract]:On the basis of llumina MiSeq sequencing platform, the effects of compost and chemical fertilizer on the fresh quality of bulbs and rhizosphere soil bacteria and fungal microbial communities of Lanzhou Lily were studied.The results showed that organic compost had a significant effect on increasing the fresh quality of lily bulb, and increased 76. 6% compared with chemical fertilizer.The results of species notes showed that the number of bacteria phylum, class, order, family, genus in compost soil was higher than that in fertilizer application soil, and the number of fungi in soil treated with chemical fertilizer was higher than that in organic fertilizer treatment.Based on the richness index (Chao1) and the diversity index (Shannon), the overall change of bacterial community was smaller than that of fungal community.The relative abundance of some bacterial genera is not obvious in the samples of organic fertilizer or chemical fertilizer. Most of the bacteria in these genera are beneficial bacteria.In addition, some beneficial endemic genera were found in organic compost samples, such as Nonomuraea, while those in chemical fertilizer group were found to be Aquicellaa.The abundance of Fusarium, Aspergillus, Penicillium and Acremoniumin in genus Fusarium, Aspergillus, Penicillium and Acremonium) decreased significantly under organic fertilizer treatment, and there were many potential or opportunistic pathogenic fungi in these genera.To some extent, the application of organic fertilizer can effectively improve the soil microbial community in lily rhizosphere.
【作者單位】： 中國(guó)科學(xué)院寒區(qū)旱區(qū)環(huán)境與工程研究所生態(tài)與農(nóng)業(yè)研究室(皋蘭生態(tài)與農(nóng)業(yè)綜合研究站);甘肅省寒區(qū)旱區(qū)逆境生理與生態(tài)重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院大學(xué);中國(guó)科學(xué)院寒區(qū)旱區(qū)環(huán)境與工程研究所沙漠與沙漠化研究室;
【基金】：國(guó)家自然科學(xué)基金(31370447) 中國(guó)科學(xué)院“百人計(jì)劃”項(xiàng)目(27Y127L41002)~~
【分類(lèi)號(hào)】：S682.29;S141.4

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