發(fā)布時間：2018-04-15 10:08

  本文選題：紅樺林 + 高通量測序�。� 參考：《環(huán)境科學(xué)》2017年07期

【摘要】：研究秦嶺辛家山林區(qū)紅樺林細(xì)菌群落在土壤剖面上的分布狀況,對評估土壤細(xì)菌在森林生態(tài)系統(tǒng)土壤肥力調(diào)節(jié)、碳氮循環(huán)等作用至關(guān)重要.采用Illumina MiSeq高通量測序技術(shù)對土壤細(xì)菌16S r DNA V3~V4可變區(qū)進(jìn)行測序,結(jié)合相關(guān)生物信息學(xué)分析,初步探討了紅樺林0~10、10~20、20~40和40~60 cm這4個土壤層細(xì)菌群落豐富度、多樣性指數(shù)和細(xì)菌群落組成及豐度變化.結(jié)果表明,在紅樺林土壤剖面上,OTUs、Chao1指數(shù)、Shannon指數(shù)均在0~10 cm處達(dá)到最大值,分別為1 688、2 314、8.66,土層間差異不顯著.4個土壤層的優(yōu)勢菌門均為酸桿菌門(Acidobacteria)、變形菌門(Proteobacteria),主要的優(yōu)勢菌屬為Gp4、Gp6和Gp16.優(yōu)勢菌門的相對豐度在土層間并不相同,0~10 cm土壤層具有較高的變形菌門(Proteobacteria),其相對豐度為23.62%,而40~60 cm具有較高的酸桿菌門(Acidobacteria),相對豐度為62.88%.酸桿菌門(Acidobacteria)與全氮、土壤有機(jī)碳、C/N、可溶性有機(jī)碳顯著相關(guān),變形菌門(Proteobacteria)與土壤含水量、土壤有機(jī)碳、可溶性有機(jī)碳顯著相關(guān).經(jīng)RDA分析證明,影響秦嶺紅樺林土壤剖面細(xì)菌群落分布的主要土壤因素是可溶性有機(jī)碳.這些研究結(jié)果表明在秦嶺紅樺林土壤4個土層均有較高的細(xì)菌多樣性,為進(jìn)一步認(rèn)識森林土壤細(xì)菌多樣性奠定了理論基礎(chǔ),在研究森林生態(tài)系統(tǒng)土壤剖面養(yǎng)分循環(huán)過程時應(yīng)予以考慮.
[Abstract]:It is very important to study the distribution of bacterial community in the soil profile of the red birch forest in Xinjiashan forest area of Qinling Mountains in order to evaluate the regulation of soil bacterial fertility and the carbon and nitrogen cycle in the forest ecosystem.The 16s r DNA V3~V4 variable region of soil bacteria was sequenced by Illumina MiSeq high-throughput sequencing technique. Combined with the bioinformatics analysis, the richness of bacterial communities in the four soil layers of the red birch forest (01010, 10, 10, 20, 20, 20, 40, 40, 60 cm) was preliminarily studied.Diversity index, composition and abundance of bacterial community.The results showed that the Shannon index of OTUsN Chao1 index reached the maximum at 0 ~ 10 cm on the soil profile of birch forest.The dominant bacteria in the four soil layers were Acidobacteria acidobacteriae, Proteobacteriae, Proteobacteria, and the main dominant bacteria were Gp4Gp6 and Gp16.The relative abundance of dominant phylum was different between soil layers, and the relative abundance of Proteobacteriae was 23.62cm, while that of 400.60cm was higher than that of Acidobacteriae, and the relative abundance was 62.88.Acidobacteria) was significantly correlated with total nitrogen, soil organic carbon C / N, soluble organic carbon, and Proteobacteria) with soil water content, soil organic carbon and soluble organic carbon.RDA analysis showed that soluble organic carbon was the main soil factor that affected the distribution of bacterial community in the soil profile of red birch forest in Qinling Mountains.These results indicated that there was high bacterial diversity in the four soil layers of the red birch forest in the Qinling Mountains, which laid a theoretical foundation for further understanding the bacterial diversity in the forest soil.The nutrient cycling process in soil profile of forest ecosystem should be considered.
【作者單位】： 西北農(nóng)林科技大學(xué)資源環(huán)境學(xué)院農(nóng)業(yè)部西北植物營養(yǎng)與農(nóng)業(yè)環(huán)境重點實驗室;陜西省寶雞市辛家山林業(yè)局;西北農(nóng)林科技大學(xué)林學(xué)院;
【基金】：國家林業(yè)公益性行業(yè)科研專項(201304307)
【分類號】：S714.3

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2 傅志軍;太白山紅樺林的排序[J];西北植物學(xué)報;1995年04期

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