本文選題：灰葡萄孢 + 侵染墊��； 參考：《植物病理學(xué)報》2016年05期

【摘要】：灰葡萄孢產(chǎn)生功能上類似附著胞的侵染墊來侵染寄主植物,但目前對于侵染墊形成的分子機制尚不明確。本文利用高通量測序技術(shù)對菌株CanBC-1(弱毒,不形成侵染墊)和CanBC-1c-66(強毒,正常形成侵染墊)進行了轉(zhuǎn)錄水平比較。結(jié)果表明:在菌株CanBC-1中共檢測到2 333個差異表達基因(與菌株CanBC-1c-66相比較),其中1 425個基因表達上調(diào),908個基因表達下調(diào)。對差異表達基因進行功能注釋(GO)分析發(fā)現(xiàn),在細胞組分(Cellular component)中細胞(Cell)和細胞部分(Cell part)這2個亞類所占比例較大,在分子功能(Molecular function)中結(jié)合活性(Binding)和催化活性(Catalytic)這2個亞類所占比例較大。這些結(jié)果暗示差異基因主要參與細胞、代謝和發(fā)育等生物學(xué)過程。篩選得到12個與真菌致病相關(guān)的基因,其中BC1G_03994和BC1G_01012與稻瘟病菌侵染相關(guān)的classⅡ疏水蛋白基因Mhp1同源。RT-PCR檢測發(fā)現(xiàn)這2個基因在弱毒菌株CanBC-1中表達下調(diào),同時該菌株疏水性降低,推測它們可能參與灰葡萄孢侵染墊形成。這些差異基因的獲得為揭示灰葡萄孢侵染形成的分子機制奠定了基礎(chǔ)。
[Abstract]:The sporulation of grapevine sporulation is similar to appressant pad to infect host plants, but the molecular mechanism of infection pad formation is not clear at present.The transcriptional levels of CanBC-1 and CanBC-1c-66 were compared with those of CanBC-1 and CanBC-1c-66 by high-throughput sequencing.The results showed that a total of 2 333 differentially expressed genes were detected in strain CanBC-1 (compared with strain CanBC-1c-66, 1 425 genes were up-regulated and down-regulated in 908 genes).The functional analysis of differentially expressed genes revealed that the two subclasses, Cell-cell and part-cell, were the most common in cellular component.The two subclasses of binding activity (binding) and catalytic activity (Catalytic) in molecular functional molecular function accounted for a large proportion.These results suggest that differential genes are involved in cellular, metabolic and developmental biological processes.Twelve genes related to fungal pathogenicity were screened, among which class 鈪，

本文編號：1741914