本文選題：敵草隆　切入點(diǎn)：降解　出處：《農(nóng)藥》2017年05期

【摘要】：[目的]篩選可降解敵草隆菌株,并研究其降解特性,為微生物降解敵草隆提供新途徑。[方法]采用平板涂布法從植物體內(nèi)分離內(nèi)生真菌,并通過搖瓶富集法篩選有降解作用的菌株;通過形態(tài)學(xué)特征和ITS鑒定菌株;采用高效液相色譜法檢測分離的內(nèi)生菌對敵草隆的降解效果,并研究其降解的最適溫度、pH值、營養(yǎng)源和降解動(dòng)力學(xué)特性。通過標(biāo)準(zhǔn)品比對鑒定降解產(chǎn)物。[結(jié)果]從牛筋草中分離得到一株具有降解除草劑敵草隆能力的真菌,命名為D12,鑒定為鐮刀菌屬(Fusarium Lk.ex Fr.)。降解條件優(yōu)化試驗(yàn)結(jié)果表明,該菌的最佳營養(yǎng)源為淀粉,在35℃和pH值6的條件下對敵草隆具有良好降解作用。以敵草隆50 mg/L為起始質(zhì)量濃度進(jìn)行研究,經(jīng)過7 d培養(yǎng),降解率達(dá)57.42%,半衰期為7.30 d。鑒定其降解產(chǎn)物為1-(3,4-dichlorophenyl)methylurea。[結(jié)論]鐮刀菌屬D12具有降解敵草隆得能力,在微生物降解除草劑領(lǐng)域具有良好的應(yīng)用前景。
[Abstract]:[objective] to screen biodegradable diolong strain and study its degradation characteristics, to provide a new way for microbial degradation of diolong.[methods] endophytic fungi were isolated from plants by plate coating method, and biodegradable strains were screened by flask enrichment method, and identified by morphological characteristics and ITS.High performance liquid chromatography (HPLC) was used to determine the degradation effect of endophytic bacteria on dimethyrolone, and the optimum temperature, pH, nutrient source and degradation kinetics were studied.The degradation products were identified by standard product comparison.[results] A fungus with the ability to degrade the herbicide diethyluron was isolated and named as D12 and identified as Fusarium Lk.ex Fr.r.The optimum degradation conditions showed that the optimum nutrient source of the strain was starch, which had a good degradation effect on diazolone at 35 鈩，

本文編號：1714519