提取方法對土壤可溶性有機碳測定結(jié)果的影響
發(fā)布時間:2018-03-29 09:17
本文選題:可溶性有機碳(DOC) 切入點:振蕩時間 出處:《生態(tài)環(huán)境學(xué)報》2017年11期
【摘要】:為驗證浸提條件對土壤可溶性有機碳(DOC)浸提量的影響,選擇具有代表性的3種土地利用方式的土壤進行實驗,研究土樣處理方式、提取時間、水土比、提取劑種類和振蕩次數(shù)等提取條件對DOC浸提量的影響。結(jié)果表明:土樣處理方式影響DOC浸提量的大小順序為:風(fēng)干過2 mm篩鮮樣過1 mm篩過2 mm篩過5 mm篩不過篩,風(fēng)干過2 mm篩的DOC提取量是鮮樣不過篩的2.4倍,且各處理之間DOC浸提量差異性達顯著水平(P0.05);DOC的浸提量隨振蕩時間變化不是特別明顯,振蕩4 h后基本達到平衡;土壤DOC浸提量隨水土比的增加而增大,呈顯著的線性關(guān)系;浸提劑種類對土壤DOC浸提量影響顯著,4種提取劑提取出來的DOC量的大小順序為:DOC(80℃,H_2O)DOC(25℃,0.5 mol·L~(-1) NaHCO_3)DOC(25℃,0.5 mol·L~(-1) K_2SO_4)DOC(25℃,H_2O);單次提取量隨提取次數(shù)增加而降低,累積提取量隨浸提次數(shù)增加而升高,且累積提取的DOC量與浸提次數(shù)呈極顯著正相關(guān)。建議采用過2 mm篩的土壤鮮樣,提取劑用25℃去離子水,水土比采用5∶1,連續(xù)振蕩4 h后一次過濾測定土壤DOC含量。本研究有助于增強不同研究結(jié)果之間DOC數(shù)據(jù)的可比性,也有利于推動土壤DOC測定標(biāo)準(zhǔn)的完善。
[Abstract]:In order to verify the effect of extraction conditions on the extraction amount of soil soluble organic carbon (SOC), three typical land use methods were selected to study the treatment of soil samples, extraction time and water / soil ratio. The effects of extraction conditions such as the type of extractant and the number of oscillations on the extraction amount of DOC were studied. The results showed that the order of soil sample treatment affecting DOC extraction was as follows: air drying over 2 mm, fresh sample was more than 1 mm, and 2 mm was more than 5 mm. The amount of DOC extracted from the air-dried screen over 2 mm was 2.4 times that of the fresh sample, and the difference of the DOC extraction amount among the treatments reached a significant level. The extraction amount of DOC was not especially obvious with the oscillating time, and the balance was basically reached after 4 hours of oscillation. The amount of DOC in soil increased with the increase of soil and water ratio, and there was a significant linear relationship. The order of DOC quantity extracted by the four extractants was: (1) DOC = 80 鈩,
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