本文選題：豬場(chǎng)沼液　切入點(diǎn)：微生物強(qiáng)化　出處：《華中農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：豬場(chǎng)沼液是豬場(chǎng)廢水經(jīng)過厭氧發(fā)酵后產(chǎn)生的具有高氨氮(NH4~(+)-N)和高化學(xué)需氧量(COD)的有機(jī)養(yǎng)殖廢水。由于沼液的低COD/N特性,微生物再利用困難,氮磷排放不達(dá)標(biāo)成為技術(shù)難題。本課題在實(shí)驗(yàn)室模擬條件下對(duì)豬場(chǎng)沼液除氨菌株、工藝條件以及生物強(qiáng)化序批式生物反應(yīng)器(SBR)工藝脫氮效果進(jìn)行了研究,主要結(jié)果如下:(1)篩選得到氨氧化細(xì)菌AT7(AOB,Nitrosomonas europaea),在起始NH4~(+)-N濃度為200 mg/L-250 mg/L的沼液中處理3 d后,NH4~(+)-N去除率達(dá)71.1%;篩選得到亞硝酸氧化細(xì)菌Y3-2(NOB,Nitrobacter winogradskyi),4 d后亞硝酸鹽(NO2~(-)-N)的積累量降低41.7%。(2)同時(shí)接種AT7和Y3-2,濃度分別為105 CFU/m L,轉(zhuǎn)速為200 r/min,吸附劑稻草粉的添加量為0.3 g/100 m L,對(duì)于NH4~(+)-N濃度為200 mg/L-250 mg/L的沼液,3 d后NH4~(+)-N去除率達(dá)77.8%;對(duì)于低NH4~(+)-N為60 mg/L以下的沼液,需添加甲醇為碳源,補(bǔ)加頻率為2 d一次,3 d后NH4~(+)-N去除率為80.4%。(3)在SBR系統(tǒng)中,采用曝氣和靜止交替進(jìn)行,作用時(shí)間分別為6 h和2 h,接種AT7、Y3-2各105 CFU/m L,3 d后NH4~(+)-N去除率為89.3%,轉(zhuǎn)化而成的硝酸鹽(NO3~(-)-N)升高39.7%。(4)調(diào)節(jié)COD/N的最適比例為11:1;以甲醇為碳源,接種硝化細(xì)菌和反硝化細(xì)菌,NH4~(+)-N、TN、COD和TP的去除率分別為98.7±0.4%,83.7±0.8%,97.4±0.2%,70.6±5.0%。低聚果糖廢液能替代甲醇,降低成本,NH4~(+)-N、TN、COD和TP的去除率分別為98.9±0.3%,95.1±1.5%,97.1±0.3%,73.9±3.9%。(5)對(duì)NH4~(+)-N濃度為771 mg/L的高濃度沼液,以系統(tǒng)出水循環(huán)利用稀釋進(jìn)水的模式,降低NH4~(+)-N濃度至200 mg/L-250 mg/L,SBR系統(tǒng)運(yùn)行21 d后穩(wěn)定,NH4~(+)-N、TN、COD和TP去除率分別為98.2±0.2%,77.8±0.5%,94.1±0.6%,77.1±1.6%。(6)室外模擬試驗(yàn)中,日平均溫度在9℃-20℃之間,NH4~(+)-N、TN、COD和TP去除率分別可達(dá)97.9±0.2%,73.8±0.9%,92.1±0.9%,80.4±1.9%。說明在一定的溫度范圍內(nèi),溫度變化對(duì)SBR系統(tǒng)的運(yùn)行沒有太大的影響。
[Abstract]:Pig farm biogas liquor is an organic culture wastewater with high ammonia nitrogen (NH4N) and high chemical oxygen demand (COD) produced by anaerobic fermentation of pig wastewater. Because of the low COD/N characteristics of the biogas, it is difficult for microorganism to reuse. It has become a technical problem that the nitrogen and phosphorus discharge is not up to standard. In this paper, the denitrification effect of bioreactor SBR and the strain of ammonia removal from biogas liquid of pig farm were studied under the condition of laboratory simulation. The main results were as follows: 1) the ammonia oxidizing bacteria AT7 AOBN Nitrosomonas europaeaan was screened. After 3 days of treatment in the biogas containing 200 mg/L-250 mg/L concentration of nitrite N, the removal rate of nitrite was 71.1; the nitrite oxidizing bacteria Y3-2NOBNitrobacter winogradskyianine 4 days later, the accumulation of nitrite nitrite no _ 2no _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2O _ 2-N was decreased after screening. AT7 and Y3-2 were inoculated at the same time with a concentration of 105 CFU/m / L, a rotational speed of 200rr / min, an amount of 0.3 g / 100 mL of adsorbent straw powder, and a removal rate of 77.8% for NH _ 4N (N = 200 mg/L-250 mg/L) for 3 days after inoculation; for biogas with low NH _ 4N concentration of less than 60 mg/L, When methanol was added as carbon source, NH _ 4N was added at a frequency of 2 days once a time for 3 days, and NH _ 4N removal efficiency was 80.4. N ~ (3). In SBR system, aeration and rest were used alternately. The optimum ratio of NH _ 4N (removal rate of nitrate-N is 89.3g) to nitrate-nitrate-no _ 3 (-N) increased 39.7g / L ~ (-1) after inoculation with AT7N _ 3-2 105 CFU/m / L ~ (-1) for 6 h and 2 h, respectively, and methanol was used as carbon source. Inoculated with nitrifying bacteria and denitrifying bacteria, the removal rates of COD and TP were 98.7 鹵0.410 鹵83.7 鹵0.810 鹵97.4 鹵0.2 and 70.6 鹵5.0 respectively. Fructose oligosaccharide waste liquid could replace methanol and reduce the cost of NH _ 4 (removal rates of -NTNCOD and TP were 98.9 鹵0.35.1 鹵95.1 鹵1.35 鹵3.93.90 鹵3.9. 5, respectively). In the outdoor simulation experiment, the NH _ 4N concentration up to 200 mg/L-250 mg 路L ~ (-1) SBR system was reduced by diluting the influent by the system, and the NH _ 4 ~ + _ 4 (COD and TP removal rates were 98.2 鹵0.22 ~ 77.8 鹵0.5 ~ 94.1 鹵77.1 鹵1.6.1 鹵1.6.6m ~ (-1) respectively) in the outdoor simulation experiment, and the NH _ 4N concentration was reduced to 200 mg/L-250 路L ~ (-1) / L ~ (-1) SBR system for 21 days. The average daily temperature is between 9 鈩，

本文編號(hào)：1660996