本文選題：薏苡　切入點：苗期抗旱性　出處：《中國農(nóng)業(yè)科學》2017年15期 　論文類型：期刊論文

【摘要】：【目的】干旱是影響薏苡生產(chǎn)的重要非生物脅迫因素,鑒定薏苡種質(zhì)資源的抗旱性,確定抗旱指標,篩選抗旱種質(zhì),促進薏苡產(chǎn)業(yè)發(fā)展。【方法】以50份薏苡種質(zhì)為材料,設置正常供水和反復干旱2個處理,在旱棚內(nèi)進行盆栽試驗,測定干旱對幼苗存活率、株高、莖粗、葉長、葉寬、地上部鮮重、地上部干重、根長、根粗、根鮮重和根干重的影響;采用抗旱性度量值(D值)、綜合抗旱系數(shù)(CDC值)、加權抗旱系數(shù)(WDC)、相關分析、頻次分析、主成分分析、灰色關聯(lián)度分析、隸屬函數(shù)分析、聚類分析和逐步回歸分析相結合的方法,對其進行苗期抗旱性鑒定及抗旱指標篩選。【結果】干旱脅迫對各指標均有極顯著影響。相關分析表明,幼苗干旱存活率與莖粗、葉寬和根粗呈極顯著正相關,與葉長、地上部鮮重、地上部干重、根長、根鮮重和根干重呈顯著正相關,與株高呈不顯著的正相關。頻次分析表明,各指標對干旱脅迫反應的敏感程度依次為根干重、根鮮重、葉寬、根粗、根長、幼苗干旱存活率、地上部干重、莖粗、地上部鮮重、葉長和株高。主成分分析表明,6個主成分可代表薏苡抗旱性91.40%的原始數(shù)據(jù)信息量。基于D值、CDC值和WDC值的供試薏苡種質(zhì)抗旱性排序相近�；疑P聯(lián)度分析表明,各指標DC值與D值間的關聯(lián)度大小依次為根干重、葉寬、根鮮重、莖粗、根粗、幼苗干旱存活率、根長、株高、地上部鮮重、地上部干重、葉長,這與各指標DC值與WDC值的密切程度基本吻合。根據(jù)D值進行聚類分析,可將供試薏苡種質(zhì)劃分為5個抗旱級別,其中Ⅰ級3份、Ⅱ級17份、Ⅲ級9份、Ⅳ級20份、Ⅴ級1份。除葉長、地上部鮮重、地上部干重和根干重外,其余指標的隸屬函數(shù)值、CDC值、D值和WDC值均隨抗旱級別的升高而增大。逐步回歸分析表明,與D值密切相關的指標有幼苗干旱存活率、株高、葉寬、根長和根鮮重。【結論】苗期抗旱性強的薏苡種質(zhì)yy18-1、yy14-3和yy13-1,可作為薏苡抗旱育種、抗旱機理及干旱調(diào)控緩解機制研究材料。幼苗存活率、株高、葉寬、根長和根鮮重可作為評價薏苡種質(zhì)資源苗期抗旱性的指標性狀。
[Abstract]:[objective] drought is an important abiotic stress factor affecting the production of Coix. The drought resistance of Coix lachryma germplasm resources was identified, the drought resistance index was determined, the drought resistant germplasm was screened, and the industry development of Coix lacryma was promoted. [methods] 50 Coix germplasms were used as materials. Two treatments, normal water supply and repeated drought, were used to test the survival rate of seedlings, plant height, stem diameter, leaf length, leaf width, fresh weight, dry weight, root length and root diameter. The effects of root fresh weight and root dry weight were analyzed by using drought resistance measure value D value, comprehensive drought resistance coefficient and CDC value, weighted drought resistance coefficient, correlation analysis, frequency analysis, principal component analysis, grey correlation analysis, membership function analysis, Cluster analysis and stepwise regression analysis were used to identify the drought resistance of seedlings and to screen drought resistance indexes. [results] drought stress had a significant effect on each index. Correlation analysis showed that seedling drought survival rate and stem diameter were significantly affected. Leaf width was positively correlated with root diameter, leaf length, shoot fresh weight, shoot dry weight, root length, root fresh weight and root dry weight, but not with plant height. The sensitivities of the indexes to drought stress were root dry weight, root fresh weight, leaf width, root diameter, root length, seedling drought survival rate, shoot dry weight, stem diameter and shoot fresh weight. Leaf length and plant height. Principal component analysis showed that the six principal components could represent the original data information of Coix seed drought resistance 91.40%. The order of drought resistance of Coix lachryma germplasm based on D value and WDC value was similar. The grey correlation analysis showed that the drought resistance of Coix lachryma germplasm was similar. The correlation degree between DC value and D value was root dry weight, leaf width, root fresh weight, stem diameter, root diameter, seedling drought survival rate, root length, plant height, aboveground fresh weight, shoot dry weight, leaf length. According to the cluster analysis of D value, Coix seed germplasm can be divided into 5 drought-resistant grades, of which 3 are 鈪，

本文編號：1617474