發(fā)布時(shí)間：2018-03-08 14:50

  本文選題：麥草畏　切入點(diǎn)：生物降解　出處：《應(yīng)用與環(huán)境生物學(xué)報(bào)》2017年02期 　論文類型：期刊論文

【摘要】：麥草畏是理想的抗除草劑轉(zhuǎn)基因工程的靶標(biāo)除草劑;發(fā)掘新的麥草畏高效降解菌株和基因具有非常重要的理論和應(yīng)用價(jià)值.從南京土壤樣品中分離到一株麥草畏高效降解菌株,命名為3-3.根據(jù)生理生化特征和16S r DNA序列相似性分析,將其初步鑒定為蒼白桿菌屬(Ochrobactrum sp.).菌株3-3在48 h內(nèi)完全降解100 mg/L的麥草畏.該菌株降解麥草畏的最適溫度為30℃,最適p H為7.0.代謝產(chǎn)物高效液相和質(zhì)譜鑒定結(jié)果表明該菌株降解麥草畏的起始步驟是脫甲基,形成沒有除草活性的3,6-二氯水楊酸(DCSA).菌株粗酶液只在NADH存在時(shí)才有麥草畏脫甲基酶活性.PCR擴(kuò)增和該菌基因組生物信息學(xué)分析表明該菌株沒有已報(bào)道的麥草畏脫甲基酶基因DMO、Mtv及Dmt或其同源序列.總之,本研究首次分離篩選到蒼白桿菌屬的麥草畏降解菌,且該菌可能存在一個(gè)新的氧化酶類麥草畏脫甲基酶基因.
[Abstract]:Is the ideal target of herbicide dicamba herbicide resistant transgenic engineering; it has a very important theoretical and application value to explore the efficient degradation of dicamba strains and new genes isolated from Nanjing soil samples. The strain of dicamba degrading strains, named as 3-3. 16S according to the physiological and biochemical characteristics and R DNA sequence similarity analysis, the preliminary identification as Ochrobactrum (Ochrobactrum sp.). 3-3 strains of dicamba completely degraded 100 mg/L within 48 h. The optimum temperature is 30 DEG C the degradation of dicamba, the optimum P H 7.0. metabolites of HPLC and mass spectrometry identification results show that the initial steps of the degradation of dicamba is demethylated, formation of 3,6- chlorine no herbicidal activity two salicylic acid (DCSA) strains. The crude enzyme only in the presence of NADH have dicamba demethylase activity of the bacteria genome.PCR amplification and bioinformatics analysis showed that the There were no reported dicamba demethylase gene DMO, Mtv and Dmt and its homologous sequences. In short, this is the first study to isolated dicamba degrading bacteria Ochrobactrum, bacteria and the possible presence of a new enzyme dicamba demethylase gene.

【作者單位】： 南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院 農(nóng)業(yè)部農(nóng)業(yè)環(huán)境微生物工程重點(diǎn)開放實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(3157010 5) 教育部新世紀(jì)優(yōu)秀人才項(xiàng)目(NCET-13-0861)資助~~
【分類號(hào)】：X172;X592
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本文編號(hào)：1584355