本文選題：UV-B　切入點(diǎn)：大豆　出處：《聊城大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：大氣中O3濃度下降,導(dǎo)致達(dá)到地面的UV-B輻射增強(qiáng),對(duì)農(nóng)田生態(tài)系統(tǒng)帶來(lái)諸多不利影響,以往的報(bào)道多著重于大豆地上部分,UV-B對(duì)地下部分及根際微生物影響的報(bào)道較少,根瘤菌和大豆是共生作用的典范,根瘤與大豆具有密切的生理關(guān)系,在研究UV-B輻射對(duì)大豆地上部、地下部及地下部及根際微生物的影響時(shí),無(wú)法忽略根瘤菌在其中起的作用。本實(shí)驗(yàn)在大田條件下,以相同遺傳背景的野生型、多根瘤和無(wú)根瘤三種大豆為材料,設(shè)置了CK、和模擬UV-B輻射增強(qiáng)15%兩個(gè)處理,展開(kāi)對(duì)UV-B輻射增強(qiáng)環(huán)境下三種大豆生物量、UV吸收物質(zhì)等生理指標(biāo)及大豆根際微生物多樣性的研究。主要結(jié)果如下:UV-B輻射增強(qiáng)使大豆UV吸收物質(zhì)和MDA含量上升,對(duì)大豆生長(zhǎng)有抑制作用,最終導(dǎo)致大豆生物量下降,產(chǎn)量下降。通過(guò)比較苗期,花期,鼓粒期大豆的生理指標(biāo)發(fā)現(xiàn)多根瘤大豆對(duì)UV-B輻射不敏感,抗UV-B脅迫能力強(qiáng)。使用高通量測(cè)序方法對(duì)根際土細(xì)菌16s DNAV4區(qū)測(cè)序,通過(guò)分析OTU(Operational Taxonomic Units,操作分類(lèi)單元),進(jìn)行對(duì)微生物多樣性的研究。結(jié)果表明:大豆根際細(xì)菌主要是以放線菌門(mén)(Actinobacteria)、酸桿菌門(mén)(Acidobacteria)、變形菌門(mén)(Proteobacteria)、擬桿菌門(mén)(Bacteroidetes)和浮霉菌門(mén)(Planctomycetes)構(gòu)成。在大豆的三個(gè)生育期,大豆根際土菌落結(jié)構(gòu)分布差異不大。而硝化螺旋菌門(mén)(Nitrospirae)等轉(zhuǎn)氨菌含量少,這與土壤中含氮量有關(guān),本次種植的土壤含氮量較低只達(dá)到國(guó)家土質(zhì)分類(lèi)標(biāo)準(zhǔn)中第三類(lèi)土。根際土壤中菌種相對(duì)含量受到UV-B的影響,但是影響效果和細(xì)菌的種類(lèi)有關(guān)。
[Abstract]:The decrease of O _ 3 concentration in the atmosphere resulted in the enhancement of UV-B radiation to the surface, which brought many adverse effects to the farmland ecosystem. The previous reports focused on the effects of UV-B on the underground and rhizosphere microbes in the aboveground part of soybean, and there were few reports about the effects of UV-B on the underground and rhizosphere microbes. Rhizobia and soybean are typical symbiosis. Rhizobia have close physiological relationship with soybean. In the study of the effects of UV-B radiation on the aboveground, underground, underground and rhizosphere microorganisms of soybean, rhizobia and rhizosphere of soybean were studied. The effect of Rhizobium on it could not be ignored. In this experiment, three kinds of soybean with the same genetic background, wild type, multiple nodule and nodule free, were used to set up CKand and simulate 15% UV-B radiation enhancement under field condition. The physiological indexes such as UV absorbents of three soybean biomass and their rhizosphere microbial diversity were studied in the environment of enhanced UV-B radiation. The main results were as follows: enhanced UV-B radiation increased the content of UV absorbents and MDA in soybean. The growth of soybean was inhibited, which resulted in the decrease of biomass and the decrease of yield. By comparing the physiological indexes of soybean at seedling stage, flowering stage and grain filling stage, it was found that multi-nodule soybean was insensitive to UV-B radiation. High throughput sequencing method was used to sequence the 16s DNAV4 region of rhizosphere bacteria. The microbial diversity of soybean rhizosphere bacteria was studied by analyzing OTU(Operational Taxonomic units and operating taxonomic units. The results showed that actinobacter teriae, actinobacteriae, Proteobacteriae, Bacteroides and Planctomycetesa were the main components of soybean rhizosphere bacteria (Actinobacterium teriae, Actinobacteria actinobacteriae, Proteobacteriae, Bacteroides) and Planctomycetesa. During the three growth periods of soybean, The distribution of colony structure of soybean rhizosphere soil was not different, but the content of nitro spirae was less, which was related to the nitrogen content in soil. The soil nitrogen content of this planting is lower than that of the third kind of soil in the national soil classification standard. The relative content of bacteria in rhizosphere soil is affected by UV-B, but the effect is related to the species of bacteria.
【學(xué)位授予單位】：聊城大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：S565.1;S154.3

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