本文選題：生物電化學體系　切入點：洛克沙胂　出處：《合肥工業(yè)大學》2017年碩士論文　論文類型：學位論文

【摘要】：洛克沙胂(Roxarsone)作為飼料添加劑被廣泛使用,畜禽經喂食后可以抑制腸道病原菌。動物體內大部分的洛克沙胂以原型隨糞便排出。未經過恰當處理的養(yǎng)殖糞便廢水會進入環(huán)境中,產生污染危險。洛克沙胂在厭氧環(huán)境中可以還原為3-氨基-4-羥基苯胂酸(HAPA)。而對其在電化學條件下的轉化機理尚不清楚。本文通過探究洛克沙胂在生物電化學體系中的生物電化學轉化過程來探究洛克沙胂生物轉化的新途徑。通過構建生物電化學污染物轉化實驗系統(tǒng),本文研究了不同底物濃度,產電條件和產電環(huán)境共三種不同條件的電化學體系來模擬不同的自然環(huán)境條件下洛克沙胂在生物電化學體系中的生物電化學轉化過程。主要結論如下:(1)富含有機物條件下洛克沙胂48 h轉化了92.5%,生成9.29μmol/L的無機砷。此時希瓦氏菌通過三種方式來轉化無機砷:氧化途徑,還原途徑和甲基化途徑。低水平有機物條件下洛克沙胂48 h轉化了92.5%,生成1.97μmol/L的無機砷。有機物水平對洛克沙胂轉化為HAPA的影響不顯著。(2)當生物量一定時,電化學體系的電極面積越大,其產電與電極面積關系越大,而陽極液中懸浮生物量影響越小;當電極面積固定時,生物量越大,其對電化學體系產電的影響就越大。洛克沙胂在電化學體系中的轉化遷移過程為:陽極液中的洛克沙胂與附著在電極上的希瓦氏菌接觸,在細胞表面被轉化為HAPA,進而釋放到陽極中,在陽極中與懸浮或附著的的希瓦氏菌接觸,在電子的作用下,HAPA在希瓦氏菌細胞內被轉化為多種砷產物。(3)在面積為6.3 cm~2-37.5 cm~2電化學串聯(lián)系統(tǒng),面積為6.3 cm~2的電極經48h洛克沙胂濃度降低至8.3μmol/L,且其8 h內轉化效率提升2.8%;此外,面積為37.5 cm~2的電極經32 h反應,陽極液內洛克沙胂已轉化完畢,且其8 h內轉化效率提升30.2%。同時,面積為25.0 cm~2-25.0 cm~2電化學并聯(lián)系統(tǒng),洛克沙胂經32 h基本轉化完成,8 h洛克沙胂轉化效率均提升21.8%。此外,50.0 cm~2-0.0 cm~2電化學并聯(lián)系統(tǒng),面積為50.0 cm~2的電極反應至32 h時洛克沙胂轉化完成,8 h洛克沙胂轉化效率提升28.4%;然而,面積為0.0 cm~2的電化學體系反應周期結束時洛克沙胂濃度降低至0.02 mmol/L,且8 h洛克沙胂轉化效率降低10%。
[Abstract]:Roxarsone (Roxarsone) is widely used as a feed additive, and livestock and poultry can inhibit intestinal pathogens after feeding. Most of roxarsone in animals is excreted in their faeces by prototyping. Waste water from untreated aquaculture dung enters the environment. Roxarsone can be reduced to 3-amino-4-hydroxyphenylarsonic acid HAPA4 in anaerobic environment, but the mechanism of its conversion under electrochemical conditions is not clear. In this paper, the mechanism of roxarsone in bioelectrochemical system is not clear. To explore a new way of biotransformation of roxarsone. In this paper, we have studied different substrate concentrations, There are three different electrochemical systems of electricity generation and electricity producing environment to simulate the bioelectrochemical transformation of roxarsone in different natural environment. The main conclusions are as follows: 1) rich in organic matter. Under the conditions of roxarsone for 48 h, 92.5% inorganic arsenic was transformed into 9.29 渭 mol/L inorganic arsenic. At this time, Shiva strain transformed inorganic arsenic in three ways: oxidation pathway. Reduction pathway and methylation pathway. Under low organic level, roxarsone was transformed into 92.5% inorganic arsenic at 48 h, resulting in inorganic arsenic of 1.97 渭 mol/L. The effect of organic level on the transformation of roxarsone to HAPA was not significant. The larger the electrode area of the electrochemical system, the greater the relationship between the electrode area and the generation of electricity, while the less the effect of suspended biomass in the anode solution is, the greater the biomass is when the electrode area is fixed. The transformation and migration of roxarsone in the electrochemical system are as follows: the roxarsone in the anodic solution is in contact with the Shiva bacteria attached to the electrode. It is converted to HAPA on the cell surface and released into the anode, where it is in contact with the suspended or attached Shiva bacteria. In a 6.3 cm~2-37.5 cm~2 electrochemical series system, the electrode with an area of 6.3 cm~2 was reduced to 8.3 渭 mol 路L ~ (-1) after 48 h roxarsone concentration, and the conversion efficiency was increased by 2.8% in 8 h. After 32 h reaction of 37.5 cm~2 electrode, roxarsone in anodic solution was converted, and the conversion efficiency was increased by 30.2% within 8 h. Meanwhile, the electrochemical parallel system with an area of 25.0 cm~2-25.0 cm~2 was obtained. The conversion efficiency of roxarsone was increased by 21.8% after 32 h basic transformation. In addition, the 50.0 cm~2-0.0 cm~2 electrochemical parallel system was used to improve the efficiency of roxarsone conversion. At the electrode area of 50.0 cm~2 to 32 h, the conversion efficiency of roxarsone was increased by 28.4when roxarsone was transformed into roxarsone at 8 h. The concentration of roxarsone decreased to 0.02 mmol / L at the end of the reaction cycle with an area of 0.0 cm~2, and the conversion efficiency of roxarsone decreased by 10% at 8 h.
【學位授予單位】：合肥工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：X713

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