本文關(guān)鍵詞： 西瓜噬酸菌 鞭毛素 趨化性 致病力　出處：《農(nóng)業(yè)生物技術(shù)學(xué)報(bào)》2017年11期 　論文類型：期刊論文

【摘要】：鞭毛是細(xì)菌的主要運(yùn)動(dòng)器官,也是一種重要的致病因子。趨化性是有運(yùn)動(dòng)能力的細(xì)菌趨向有益刺激,逃避有害刺激的反應(yīng)。前期研究表明,缺乏趨化性核心基因che A的西瓜噬酸菌(Acidovorax citrulli)致病力下降;為明確鞭毛素基因和趨化性核心基因同時(shí)缺失對(duì)西瓜噬酸菌的影響以及趨化性與鞭毛基因間的關(guān)系,本研究構(gòu)建了西瓜噬酸菌雙缺失突變菌株ΔcheAΔfliC及其互補(bǔ)菌株ΔcheAΔfliC::che A::fliC、突變菌株ΔfliC及互補(bǔ)菌株ΔfliC::fliC,并對(duì)致病力與生物學(xué)特性進(jìn)行測(cè)定。結(jié)果顯示,雙缺失突變菌株ΔcheAΔfliC致病力、運(yùn)動(dòng)能力、生長能力、種子粘附能力均顯著降低,生物膜形成能力顯著增強(qiáng);與ΔfliC和ΔcheA單缺失突變株相比,雙缺失突變菌株ΔcheAΔfliC致病力顯著下降,生物膜形成能力顯著提升,說明fliC和che A同時(shí)缺失對(duì)西瓜噬酸菌致病力和生物膜形成能力影響更為顯著;三者運(yùn)動(dòng)能力基本保持一致,說明fliC缺失與che A缺失同樣影響西瓜噬酸菌的運(yùn)動(dòng)能力,進(jìn)一步說明了西瓜噬酸菌趨化性核心基因che A通過調(diào)控鞭毛的運(yùn)動(dòng)從而影響西瓜噬酸菌的運(yùn)動(dòng)能力。ΔfliC、ΔcheAΔfliC突變株種子上粘附能力顯著下降,且均低于ΔcheA,說明鞭毛素基因fliC對(duì)西瓜噬酸菌粘附能力起著重要作用,這將對(duì)瓜類細(xì)菌性果斑病的防控和有效防治藥劑的研制提供了基礎(chǔ)。
[Abstract]:Flagella is the main movement organ of bacteria and an important pathogenic factor. Chemotaxis is the tendency of bacteria with the ability to move towards beneficial stimulation and escape from harmful stimuli. The pathogenicity of Acidovorax citrullii, which lacks che A, was decreased, and the relationship between chemotaxis and flagellin gene and flagellin gene in watermelon phagocytic bacteria was studied in order to clarify the effect of both flagellin gene and chemoattractant core gene deletion on watermelon phagocytic bacteria, and the relationship between chemotaxis and flagellin gene. In this study, the double deletion mutant 螖 cheA 螖 fliC and its complementary strain 螖 cheA 螖 fliC::che A: 1: fliC, the mutant 螖 fliC and the complementary strain 螖 fliCw: fliCwere constructed. The pathogenicity and biological characteristics of the double deletion mutant 螖 cheA 螖 fliC were determined. The results showed that the mutant 螖 cheA 螖 fliC was virulent and athletic. Compared with 螖 fliC and 螖 cheA single deletion mutants, the virulence of double deletion mutant 螖 cheA 螖 fliC was significantly decreased, and the biofilm formation ability was significantly increased. The results showed that the effects of fliC and che A deficiency on pathogenicity and biofilm formation ability of watermelon were more significant, and the motor ability of the three was basically the same, which indicated that fliC deficiency and che A deficiency also affected the ability of watermelon phagocytomytic bacteria. The results further indicated that the chemoattractant core gene cheA of watermelon phagocytic bacteria affected the motility of watermelon phagocytic bacteria by regulating the movement of flagella. The adhesion ability of 螖 fliC, 螖 cheA 螖 fliC mutants decreased significantly. All of them were lower than 螖 cheA, indicating that flagellin gene fliC played an important role in the adhesion of watermelon phagocytic bacteria, which would provide a basis for the prevention and control of melon bacterial fruit spot disease and the development of effective control agent.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院植物保護(hù)研究所/植物病蟲害生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室;吉林農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：國家西甜瓜產(chǎn)業(yè)技術(shù)體系(No.CARS-26) 北京市自然科學(xué)基金(No.6162023) 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程項(xiàng)目 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(No.201003066)
【分類號(hào)】：S432.4

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