本文關(guān)鍵詞： 畜禽養(yǎng)殖場 腸桿菌 多粘菌素E 多重耐藥性 質(zhì)粒　出處：《生態(tài)環(huán)境學(xué)報(bào)》2017年04期 　論文類型：期刊論文

【摘要】：畜禽養(yǎng)殖業(yè)大量使用抗生素引起了廣泛的微生物耐藥性問題,研究抗生素耐藥菌株可以了解環(huán)境中細(xì)菌的耐藥性情況和抗性基因的傳播機(jī)制。采集畜禽養(yǎng)殖場周邊的污水,分析了污水中多粘菌素E耐藥性細(xì)菌和耐藥性腸桿菌的豐度;同時(shí)以一株具有較高多粘菌素E耐藥水平的腸桿菌R11為例,首先進(jìn)行16S r DNA測序和抗生素耐藥譜分析,然后通過質(zhì)粒提取、酶切電泳以及脈沖場凝膠電泳(PFGE),分析其內(nèi)源性質(zhì)粒等情況。結(jié)果表明:在畜禽養(yǎng)殖場周邊污水環(huán)境中,多粘菌素E耐藥性細(xì)菌占細(xì)菌總數(shù)的0.14%~0.35%,多粘菌素E耐藥性腸桿菌占腸桿菌總數(shù)的1.50%~7.40%,而腸桿菌占細(xì)菌總數(shù)的1.13%~2.45%,說明腸桿菌可能是攜帶多粘菌素E抗性基因的主要細(xì)菌種類,同時(shí)也是養(yǎng)殖業(yè)污水中細(xì)菌的主要組成部分。16S r DNA序列分析確定R11是一株Enterobacter cloacae,抗生素耐藥譜分析R11具有多重耐藥性,除對林可霉素敏感外,阿米卡星、四環(huán)素和環(huán)丙沙星的最小抑菌濃度(MICs)為10μg?m L~(-1),阿莫西林的MICs為25μg?m L~(-1),磺胺甲惡唑的MICs為32μg?m L~(-1),氨芐西林的MICs為40μg?m L~(-1),鏈霉素的MICs為90μg?m L~(-1),而多粘菌素E的MICs最高,達(dá)到96μg?m L~(-1)。質(zhì)粒提取酶切和PFGE分離鑒定結(jié)果顯示,R11菌株可能有多個(gè)內(nèi)源性質(zhì)粒。綜上所述,R11菌株具有較高水平的多粘菌素E耐藥性,并且具有多重耐藥性和多個(gè)內(nèi)源性質(zhì)粒,對于研究多粘菌素E耐藥性機(jī)制具有一定的參考價(jià)值。
[Abstract]:The extensive use of antibiotics in livestock and poultry breeding has caused a wide range of microbial drug resistance problems. The study of antibiotic resistant strains can understand the drug resistance of bacteria in the environment and the transmission mechanism of resistance genes, and collect sewage from livestock and poultry farms. The abundance of polymyxin E resistant bacteria and resistant Enterobacter spp in sewage was analyzed, and the 16s r DNA sequencing and antibiotic resistance spectrum analysis were carried out with a high polymyxin E resistant Enterobacter coli R11 as an example. Then the plasmid extraction, enzyme digestion electrophoresis and pulse field gel electrophoresis were used to analyze the endogenous plasmids. The results showed that: in the sewage environment around the livestock and poultry farms, Polymyxin E resistant bacteria accounted for 0.14% of the total number of bacteria, polymyxin E resistant Enterobacter accounted for 1.50% of the total number of Enterobacter, and Enterobacter accounted for 1.133.45% of the total number of bacteria, indicating that Enterobacter may be the main type of bacteria carrying polymyxin E resistance gene. At the same time, it is also the main component of bacteria. 16s r DNA sequence analysis confirmed that R11 was a strain of Enterobacter cloacae.The antibiotic resistance spectrum analysis showed that R11 was multidrug resistant, except for lincomycin sensitive, amikacin. The minimum inhibitory concentrations of tetracycline and ciprofloxacin were 10 渭 g? The MICs of amoxicillin is 25 渭 g? The MICs of sulfamethoxazole is 32 渭 g? The MICs of ampicillin is 40 渭 g? The MICs of streptomycin is 90 渭 g? The MICs of polymyxin E was the highest, reaching 96 渭 g? The results of restriction endonuclease digestion and PFGE isolation showed that the strain R11 might have multiple endogenous plasmids. In conclusion, the strain R11 had high level of polymyxin E resistance, and had multiple multidrug resistance and multiple endogenous plasmids. It has certain reference value for studying the mechanism of polymyxin E resistance.
【作者單位】： 山東建筑大學(xué)市政與環(huán)境工程學(xué)院;山東省醫(yī)學(xué)科學(xué)院山東省醫(yī)藥生物技術(shù)研究中心;
【基金】：國家自然科學(xué)基金項(xiàng)目(31500094) 山東省自然科學(xué)基金項(xiàng)目(ZR2015EM018) 山東省醫(yī)學(xué)科學(xué)院醫(yī)藥衛(wèi)生科技創(chuàng)新工程項(xiàng)目(201604)
【分類號】：X172;X713

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 袁越,劉修才,顧孝誠;多粘菌素-B的溶液構(gòu)象研究[J];生物物理學(xué)報(bào);1997年03期

2 ;抗噬菌體多粘菌素E發(fā)酵菌株的篩選[J];北京大學(xué)學(xué)報(bào)(自然科學(xué));1973年00期

3 魏家綿;多粘菌素B對類囊體膜上M_g~(2+)-ATPase功能的影響及其作用部位[J];植物生理學(xué)報(bào);1990年04期

4 郭剛,王源,劉俊康,肖湘,徐啟旺;多粘菌素B Sub—MICs導(dǎo)致大腸桿菌自溶的研究[J];中國微生態(tài)學(xué)雜志;1997年02期

5 蔡玲民;;多粘菌素B對革蘭氏陰性菌菌苗中的脂多糖的滅活作用[J];國外醫(yī)學(xué).生物制品分冊;1982年04期

6 ;[J];;年期

，

本文編號：1552008