發(fā)布時(shí)間：2018-02-20 02:15

  本文關(guān)鍵詞： 寡營(yíng)養(yǎng) 解磷菌 解磷特性 生物磷肥　出處：《河南農(nóng)業(yè)科學(xué)》2017年08期 　論文類型：期刊論文

【摘要】：為獲得更加適應(yīng)寡營(yíng)養(yǎng)的自然環(huán)境條件的解磷菌株,以1/4蒙金娜培養(yǎng)基從土壤中分離篩選高效解磷菌株,并對(duì)其進(jìn)行鑒定,分析其生長(zhǎng)條件及解磷機(jī)制。結(jié)果表明,從土壤中共分離獲得解磷菌9株,通過(guò)對(duì)解磷量的定量測(cè)定確定高效解磷菌株XMT-5,其最大解磷量為195.63 mg/L;從菌落特征、生理生化特性及16S rRNA基因序列分析三方面鑒定菌株XMT-5為根瘤菌(Rhizobium sp.);菌株XMT-5在寡營(yíng)養(yǎng)條件下可以分泌檸檬酸、酒石酸和乙酸3種有機(jī)酸,從而可使培養(yǎng)液pH值下降;菌株XMT-5可在溫度4~42℃、pH值4~10、Na Cl質(zhì)量濃度0~80 g/L以及寡營(yíng)養(yǎng)條件下生長(zhǎng),環(huán)境適應(yīng)能力較強(qiáng),具有良好的應(yīng)用前景。
[Abstract]:In order to obtain more suitable for the natural environment condition of oligonutrition, the high efficiency phosphorus releasing bacteria strains were isolated and screened from the soil in 1/4 mongola medium, and identified, the growth conditions and the mechanism of phosphorus release were analyzed, the results showed that: 1. Nine strains of phosphorus releasing bacteria were isolated from soil, and XMT-5 was determined by quantitative determination of the amount of phosphorus. The maximum amount of phosphorus release was 195.63 mg / L. Physiological and biochemical characteristics and 16s rRNA gene sequence analysis showed that the strain XMT-5 was identified as Rhizobium sp.0.The strain XMT-5 could secrete three organic acids, citric acid, tartaric acid and acetic acid, which decreased the pH value of the culture medium. The strain XMT-5 could grow under the conditions of temperature 442 鈩，

本文編號(hào)：1518525