本文關(guān)鍵詞： 再生水 室內(nèi)模擬 微生物數(shù)量 群落結(jié)構(gòu) 化學性質(zhì)　出處：《哈爾濱師范大學》2015年碩士論文　論文類型：學位論文

【摘要】：再生水是指廢水經(jīng)過濾、沉淀、混凝等處理后,達到規(guī)定的水質(zhì)指標,滿足特定的使用要求,在一定范圍內(nèi)能夠再次被有益利用的水資源,普遍應用于工業(yè)、農(nóng)業(yè)、城市服務業(yè)、景觀環(huán)境和補充地下水源等。本研究采用室內(nèi)土柱模擬的實驗手段,以自來水澆灌為對照組,再生水回用為實驗組,研究再生水回用對土壤化學性質(zhì)、微生物數(shù)量和微生物群落結(jié)構(gòu)的影響,并深入探討土壤化學性質(zhì)與微生物數(shù)量之間的相關(guān)關(guān)系、土壤化學性質(zhì)和微生物群落結(jié)構(gòu)的響應關(guān)系,以期對再生水回用安全評價及改進再生水處理工藝提供理論支持。通過對自來水和再生水澆灌區(qū)6項土壤樣品化學性質(zhì)(有機質(zhì)、全氮、總磷、速效磷、pH值、含水量)進行測試分析,明確再生水回用過程中土壤化學性質(zhì)的變化規(guī)律。結(jié)果表明:隨再生水回用量的增加,土壤有機質(zhì)(OM)和全氮(TN)含量呈升高趨勢,推測再生水回用可增加土壤養(yǎng)分和提高土壤肥力;再生水回用對土壤總磷(TP)和速效磷(AP)含量無顯著影響,均在土壤元素安全含量范圍內(nèi),未造成磷元素的聚集;再生水回用對土壤pH值和含水量無顯著影響,不會引起土壤酸堿化。通過傳統(tǒng)微生物培養(yǎng)法,研究再生水回用下土壤細菌、放線菌、真菌總數(shù)和群落結(jié)構(gòu)的變化規(guī)律,結(jié)果表明:隨再生水回用量的增加,表層0~20 cm土壤細菌和放線菌數(shù)量呈升高趨勢,而20~40 cm和40~60 cm土層細菌、放線菌、真菌數(shù)量無顯著變化,各土層微生物數(shù)量表現(xiàn)為:0~20 cm土層20~40 cm土層40~60 cm土層,且差異顯著;再生水回用區(qū)分離出可培養(yǎng)細菌分布于15個屬,其中不動桿菌屬(Acinetobacter sp.)為優(yōu)勢菌屬,氣球菌屬(Aerococcus sp.)、鹽桿菌屬(Halobacterium sp.)等6個屬為特有菌屬;再生水回用區(qū)分離出可培養(yǎng)放線菌分布于9個屬,其中鏈霉菌屬(Streptomyces sp.)為優(yōu)勢菌屬,游動放線菌屬(Actinoplanaceae sp.)、束絲放線菌屬(Actinosynnema sp.)為特有菌屬;再生水回用區(qū)分離出可培養(yǎng)真菌分布于12個屬,其中青霉屬(Penicillium sp.)為優(yōu)勢菌屬,帚霉屬(Scopulariopsis sp.)、囊軸霉屬(Rhipidium sp.)和枝孢霉屬(Cladosporium sp.)為特有菌屬。通過SPSS17.0(統(tǒng)計產(chǎn)品與服務解決方案,Statistical Product and Service Solutions)對土壤化學性質(zhì)與微生物數(shù)量之間的相關(guān)性分析表明,細菌、放線菌、真菌總數(shù)與有機質(zhì)(OM)、全氮(TN)、總磷(TP)、速效磷(AP)呈正相關(guān);通過CANOCO4.5去趨勢分析(DCA)和冗余分析(RDA)研究土壤化學性質(zhì)與微生物類群之間的對應關(guān)系,結(jié)果表明,土壤微生物群落結(jié)構(gòu)隨著化學性質(zhì)的變化表現(xiàn)出一定的空間差異,有機質(zhì)(OM)、全氮(TN)、總磷(TP)、速效磷(AP)是對微生物群落結(jié)構(gòu)影響較大的理化因子,其中有機質(zhì)(OM)、全氮(TN)和速效磷(AP)含量對動性球菌屬(Planococcus sp.)、鹽桿菌屬(Halobacterium sp.)、束絲放線菌屬(Actinosynnema sp.)、游動放線菌屬(Actinoplanaceae sp.)的分布影響較大,總磷(TP)含量對奈瑟氏球菌屬(Neisseria sp.)的分布影響較大。
[Abstract]:Reclaimed water refers to the water resources which can be used again in a certain range after being treated by filtration, sedimentation, coagulation and so on, so as to meet the specified water quality targets and meet the specific requirements of use. They are widely used in industry and agriculture. Urban service industry, landscape environment and supplementary underground water sources were used in this study. The chemical properties of soil were studied by indoor soil column simulation, using tap water irrigation as control group and reclaimed water as experimental group. The relationship between soil chemical properties and microbial population, and the response relationship between soil chemical properties and microbial community structure were also discussed. In order to provide theoretical support for safety evaluation of reclaimed water reuse and improvement of reclaimed water treatment process, the chemical properties of 6 soil samples (organic matter, total nitrogen, total phosphorus, available phosphorus and pH value of available phosphorus) in tap water and reclaimed water irrigated area were studied. The results showed that the content of soil organic matter (OM) and total nitrogen (TNN) increased with the increase of the amount of reclaimed water, and the content of soil organic matter (Om) and total nitrogen (TNN) increased with the increase of the amount of reclaimed water. It was inferred that reclaimed water reuse could increase soil nutrients and soil fertility, and that reclaimed water reuse had no significant effect on the contents of total phosphorus (TP) and rapidly available phosphorus (APP), but did not result in the accumulation of phosphorus in the safe range of soil elements. Reclaimed water reuse had no significant effect on soil pH value and water content, and would not cause soil acid-alkalification. The changes of soil bacteria, actinomycetes, fungi and community structure under reclaimed water reuse were studied by traditional microbial culture method. The results showed that the amount of bacteria and actinomycetes increased with the increase of reclaimed water reclaimed water, but the number of bacteria, actinomycetes and fungi did not change significantly in the soil layer of 20 ~ 40 cm and 40 ~ 60 cm. The number of microbes in each soil layer was 4060 cm in 20 ~ 40 cm soil layer, and the difference was significant, and the culturable bacteria were isolated and distributed in 15 genera in reclaimed water reuse area, among which Acinetobacter sp. (Acinetobacter sp.) was the dominant genus. Aerococcus sp. sp. and Halobacterium sp.) are endemic genera, and culturable actinomycetes have been isolated from the reclaimed water reuse area in 9 genera, among which Streptomyces sp. is the dominant genus. The genus Actinoplanaceae sp. and Actinosynnema sp. are endemic to the genus Actinoplanaceae, and 12 genera of culturable fungi have been isolated from the reclaimed water reuse area, among which Penicillium sp. is the dominant genus. Scopulariopsis sp., Rhipidium sp. and Cladosporium sp.) are endemic to the genus Scopulariopsis. The total number of fungi was positively correlated with organic matter (CANOCO4.5), total nitrogen (TNN), total phosphorus (TP), available phosphorus (APP), and the relationship between soil chemical properties and microbial community was studied by using CANOCO4.5 detrend analysis (DCAA) and redundancy analysis (RDAA). The soil microbial community structure showed some spatial differences with the change of chemical properties. Organic matter (OMN), total nitrogen (TNN), total phosphorus (TP) and available phosphorus (APP) were the most important physical and chemical factors affecting the microbial community structure. The contents of OMN, TNN and APP) affected the distribution of Actinosynnema sp. and Actinoplanaceae sp. in the genus Planococcus sp., Halobacterium sp., Actinosynnema sp. and Actinoplanaceae sp., respectively. The distribution of Neisseria sp. was significantly affected by the content of TPTP.
【學位授予單位】：哈爾濱師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：S154.3;S153

【參考文獻】

相關(guān)期刊論文 前2條

1 趙忠明;陳衛(wèi)平;焦文濤;王美娥;;再生水灌溉對土壤性質(zhì)及重金屬垂直分布的影響[J];環(huán)境科學;2012年12期

2 王倩;鄒志紅;;BP神經(jīng)網(wǎng)絡在再生水補給密云水庫水質(zhì)評價中的應用[J];環(huán)境科學學報;2014年09期

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本文編號：1494759