本文關(guān)鍵詞： 棉花 種子萌發(fā) 干旱脅迫 油菜素內(nèi)酯 NAC轉(zhuǎn)錄因子 基因表達(dá)　出處：《新疆農(nóng)業(yè)科學(xué)》2017年08期 　論文類(lèi)型：期刊論文

【摘要】：【目的】研究干旱脅迫下油菜素內(nèi)酯浸種棉花種子萌發(fā)期NAC轉(zhuǎn)錄因子的表達(dá)變化,分析NAC轉(zhuǎn)錄因子在油菜素內(nèi)酯(brassinolide,BR)調(diào)控棉花抗旱中的作用。【方法】根據(jù)陸地棉轉(zhuǎn)錄因子NAC家族的基因序列,擴(kuò)增獲得棉花新陸早17號(hào)NAC家族中的6個(gè)NAC基因(Gh NAC1-6)。實(shí)時(shí)熒光定量PCR檢測(cè)干旱脅迫下不同油菜素內(nèi)酯浸種后,棉花種子萌發(fā)期子葉和胚根NAC基因的表達(dá)變化�！窘Y(jié)果】棉花子葉中的Gh NAC1在干旱脅迫下受不同濃度油菜素內(nèi)酯的抑制,Gh NAC2和Gh NAC3受0.25 mg/L油菜素內(nèi)酯誘導(dǎo)表達(dá),Gh NAC5只受0.5 mg/L油菜素內(nèi)酯誘導(dǎo)表達(dá),而Gh NAC4和Gh NAC6表達(dá)無(wú)顯著變化。在棉花胚根中,Gh NAC1同樣受不同濃度油菜素內(nèi)酯的抑制,Gh NAC2和Gh NAC3受0.25 mg/L油菜素內(nèi)酯誘導(dǎo),同時(shí)Gh NAC3在2 mg/L油菜素內(nèi)酯浸種也呈現(xiàn)高表達(dá),Gh NAC5無(wú)顯著變化,Gh NAC4和Gh NAC6則受高濃度油菜素內(nèi)酯誘導(dǎo)表達(dá)。【結(jié)論】棉花種子萌發(fā)期在干旱脅迫下,棉花種子萌發(fā)期子葉和胚根的Gh NAC1的表達(dá)受油菜素內(nèi)酯抑制,Gh NAC2和Gh NAC3受低濃度油菜素內(nèi)酯的表達(dá)誘導(dǎo),油菜素內(nèi)酯能夠影響Gh NAC的表達(dá)響應(yīng)干旱脅迫。
[Abstract]:[objective] to study the expression of NAC transcription factors during germination of cotton seeds soaked with rapesinolide under drought stress. The role of NAC transcription factors in the regulation of cotton drought resistance by Brassinolide (Br) was analyzed. [methods] according to the gene sequence of NAC family of Upland cotton transcription factors, Six NAC genes, Gh NAC1-6, were amplified from the NAC family of Xinluzao 17 cotton. Real-time quantitative PCR was used to detect the seed soaking of different rapesinolactone under drought stress. The expression of NAC gene in cotyledon and radicle of cotton seeds during germination period. [results] under drought stress, Gh NAC1 in cotyledons was inhibited by different concentrations of Brassinolide. Gh NAC2 and Gh NAC3 were induced to express Gh NAC5 by 0.25 mg/L Brassinolide. The expression was induced by only 0.5 mg/L rapesinolide. However, the expression of Gh NAC4 and Gh NAC6 did not change significantly. In cotton radicle roots, Gh NAC1 was also inhibited by different concentrations of Brassinolide, and Gh NAC2 and Gh NAC3 were also induced by 0. 25 mg/L Brassinolide. At the same time, the expression of Gh NAC3 in seed soaked with 2 mg/L Brassinolide also showed no significant change. Gh NAC4 and Gh NAC6 were induced by high concentration of rapesinolide. [conclusion] during germination of cotton seeds, the expression of Gh NAC4 and Gh NAC6 was under drought stress. The expression of Gh NAC1 in cotyledon and radicle of cotton seeds was inhibited by Brassinolide to induce the expression of Gh NAC2 and Gh NAC3 by low concentration of Brassinolide. Brassinolide could affect the expression of Gh NAC in response to drought stress.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院/新疆生物資源基因工程重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金-新疆聯(lián)合基金重點(diǎn)項(xiàng)目“棉花水分高效利用的關(guān)鍵基因的挖掘及抗旱種質(zhì)材料的創(chuàng)制”(U1303282)~~
【分類(lèi)號(hào)】：S423;S562
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本文編號(hào)：1494072