發(fā)布時間：2018-02-03 16:29

  本文關(guān)鍵詞： 二氯喹啉酸 玉米芯、竹炭及油枯復(fù)合載體 固定化 生物修復(fù)　出處：《應(yīng)用生態(tài)學(xué)報》2017年02期 　論文類型：期刊論文

【摘要】：采用玉米芯、竹炭及油枯吸附-海藻酸鈉包埋對分離到的施氏假單胞菌PFS-4進(jìn)行復(fù)合固定.采用正交試驗對固定化條件進(jìn)行優(yōu)化,研究了固定化菌劑及游離菌體對二氯喹啉酸的降解效果.結(jié)果表明:固定化菌劑制備的最佳條件為:海藻酸鈉質(zhì)量分?jǐn)?shù)為4%、吸附載體比例(玉米芯∶竹炭∶油枯)為1∶2∶1、CaCl_2質(zhì)量分?jǐn)?shù)為3%、交聯(lián)時間4 h.固定化菌劑在溫度為30℃、初始pH=7的條件下,經(jīng)6 d培養(yǎng)后,對濃度為800 mg·L~(-1)的二氯喹啉酸降解率為91.4%,而游離菌體的降解率為72.8%.將游離菌體和固定化菌劑用于實際污水及土壤處理時,固定化菌劑對水中及土壤中二氯喹啉酸去除率仍能分別達(dá)到84.2%和74.3%.研究結(jié)果表明,載體及其聯(lián)結(jié)方式對土壤中二氯喹啉酸去除產(chǎn)生顯著影響,翻動頻率與土壤中二氯喹啉酸的去除率呈顯著正相關(guān).因此,玉米芯、竹炭及油枯吸附-海藻酸鈉復(fù)合固定施氏假單胞菌PFS-4對不良環(huán)境具有較好的緩沖性能,對二氯喹啉酸污染水體及土壤原位生態(tài)修復(fù)具有潛力.
[Abstract]:Corn cob, bamboo charcoal and oil withered adsorption-sodium alginate were used to immobilize the isolated Pseudomonas sp. PFS-4. Orthogonal experiment was used to optimize the immobilization conditions. The degradation of dichloroquinolinic acid by immobilized bacteria and free bacteria was studied. The results showed that the optimum conditions for the preparation of immobilized bacteria were as follows: sodium alginate 4%. The adsorption carrier ratio (corn cob: bamboo charcoal: oil withered) was 1: 2: 1: 1 CaCl2 mass fraction was 3, crosslinking time was 4 h. The immobilized bacteria agent was immobilized at 30 鈩，

本文編號：1487837