本文關(guān)鍵詞： ABP9基因 轉(zhuǎn)基因玉米 抗旱性 田間表型 營養(yǎng)生長和生殖生長階段 籽粒產(chǎn)量　出處：《中國農(nóng)業(yè)科學(xué)院》2016年博士論文　論文類型：學(xué)位論文

【摘要】：干旱在中國是嚴(yán)重限制植物生產(chǎn)力的主要環(huán)境因素,往往給農(nóng)業(yè)造成巨大的經(jīng)濟損失。隨著全球氣候的變化,世界各地的農(nóng)業(yè)生產(chǎn)面臨著來自頻繁極端天氣條件的嚴(yán)重威脅。開發(fā)植物新品種,可以緩解環(huán)境壓力對維持產(chǎn)量穩(wěn)定的負(fù)面影響,是維持和增加農(nóng)業(yè)生產(chǎn)的關(guān)鍵。玉米(Zea mays L.)是中國和世界的一種主要作物。干旱脅迫對其產(chǎn)量和產(chǎn)量的穩(wěn)定性有很大的影響。提高玉米抗旱性已成為私人和公共部門的最優(yōu)先考慮的問題之一。前期我們從玉米中克隆了轉(zhuǎn)錄因子ABP9基因,其過量表達可提高轉(zhuǎn)基因擬南芥對包括干旱在內(nèi)的多種逆境的抗性。為研究該基因在玉米中的功能,我們創(chuàng)制了過量表達ABP9基因的玉米。在本研究中,我們評估了不同啟動子驅(qū)動ABP9表達的四種建構(gòu)即Pabp9-ABP9.1,Pubi-ABP9.1,Pubi-ABP9.2和Pubi-ABP9-3xFLAG的轉(zhuǎn)基因玉米植株在營養(yǎng)生長和生殖生長階段大田干旱下以及正常灌溉下的田間表現(xiàn)(2015),并鑒定了多個表現(xiàn)強抗旱性的轉(zhuǎn)基因事件。在營養(yǎng)生長期施加的干旱下,Pabp9-ABP9.1轉(zhuǎn)基因事件201、206、212和214,Pubi-ABP9.1轉(zhuǎn)基因事件611,612,616和617,Pubi-ABP9.2轉(zhuǎn)基因事件707、714和717,以及Pubi-ABP9-3xFLAG轉(zhuǎn)基因事件809和815與非轉(zhuǎn)基因植株相比具有較高的葉綠素?zé)晒?植被覆蓋指數(shù)提高,雌雄開花間隔期縮短,穗粒數(shù)、百粒重、平均穗籽粒產(chǎn)量增加。生殖生長期施加的干旱下,在Pabp9-ABP9.1轉(zhuǎn)基因事件201、202、204和214,Pubi-ABP9.1轉(zhuǎn)基因事件617和618,Pubi-ABP9.2轉(zhuǎn)基因事件707和714,以及Pubi-ABP9-3xFLAG轉(zhuǎn)基因事件808和817中也觀察到了同樣的表型。在正常灌溉條件下,Pabp9-ABP9.1轉(zhuǎn)基因事件201、210、212和214,Pubi-ABP9.1轉(zhuǎn)基因事件611、617、612和618,Pubi-ABP9.2轉(zhuǎn)基因事件702、707、714和717,Pubi-ABP9-3xFLAG轉(zhuǎn)基因事件803、807、808和817與非轉(zhuǎn)基因植株相比也表現(xiàn)出改善的性狀,如高葉綠素?zé)晒夂椭脖桓采w指數(shù),短雌雄開花間隔期,穗粒數(shù)、百粒重、平均穗籽粒產(chǎn)量提高。總之,本研究中鑒定的玉米轉(zhuǎn)基因事件為進一步分析玉米中ABP9基因作用的分子機制及培育耐旱生物技術(shù)產(chǎn)品提供了寶貴資源。
[Abstract]:Drought is a major environmental factor that severely limits plant productivity in China, often causing huge economic losses to agriculture. Agricultural production around the world faces serious threats from frequent extreme weather conditions. The development of new plant varieties can alleviate the negative impact of environmental pressure on maintaining yield stability. Is the key to maintaining and increasing agricultural production. Zea mays L. Drought stress is a major crop in China and the world. Drought stress has a great impact on yield and yield stability. Improving drought resistance of maize has become one of the top priorities in the private and public sectors. The transcription factor ABP9 gene was cloned in maize. Overexpression can enhance the resistance of transgenic Arabidopsis thaliana to various stresses including drought. In order to study the function of this gene in maize we have created a maize with overexpression of ABP9 gene. We evaluated four constructs of ABP9 expression driven by different promoters, namely, Pabp9-ABP9.1 / Pubi-ABP9.1. Field performance of transgenic Maize plants of Pubi-ABP9.2 and Pubi-ABP9-3xFLAG under Field drought and normal Irrigation during vegetative and Reproductive growth. (2015). The transgenic events of Pabp9-ABP9.1 in vegetative growth period were identified, and 201,206,212 and 214 transgenic events of Pabp9-ABP9.1 were identified. Pubi-ABP9.1 transgene events 611,612,616 and 617 Pubi-ABP9.2 transgenic events 707,714 and 717. The Pubi-ABP9-3xFLAG transgenic events 809 and 815 had higher chlorophyll fluorescence, higher vegetation cover index and shorter flowering interval than those of non-transgenic plants. The number of grains per panicle, 100-grain weight and average grain yield per panicle increased. Under drought during reproductive growth, Pabp9-ABP9.1 transgenic events 201,202,204 and 214were observed. Pubi-ABP9.1 transgene events 617 and 618 Pubi-ABP9.2 transgenic events 707 and 714. The same phenotype was observed in Pubi-ABP9-3xFLAG transgene events 808 and 817 under normal irrigation conditions. Pabp9-ABP9.1 transgene events 201,210,212 and 214m Pubi-ABP9.1 transgenic events 611,617,612 and 618. Pubi-ABP9.2 transgenic event 702,707,714 and 717 Pubi-ABP9-3xFLAG transgenic event 803,807. Compared with the non-transgenic plants, 808 and 817 also showed improved characters, such as high chlorophyll fluorescence and vegetation cover index, short flowering interval between male and female, number of grains per ear and 100 kernel weight. In conclusion, the transgenic events identified in this study provided valuable resources for further analysis of the molecular mechanism of ABP9 gene function in maize and the cultivation of drought-tolerant biotechnology products.
【學(xué)位授予單位】：中國農(nóng)業(yè)科學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：S513;S423
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本文編號：1479945