本文關(guān)鍵詞：北方溫帶森林不同海拔梯度土壤有機(jī)質(zhì)分解酶活性及動(dòng)力學(xué)特征研究　出處：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 溫帶森林 土壤酶 酶動(dòng)力學(xué) 碳礦化 溫度敏感性

【摘要】：本文以老禿頂南坡沿海拔梯度(500-1306 m)不同植被類型下的土壤為研究對(duì)象,旨在通過(guò)對(duì)不同海拔梯度的土壤酶活性、土壤碳礦化速率、土壤酶動(dòng)力學(xué)參數(shù)及溫度敏感性的研究,并利用相關(guān)分析、冗余分析及主成分分析等數(shù)學(xué)分析方法研究了其與土壤理化性質(zhì)的關(guān)系。探究影響不同海拔梯度土壤酶變化的關(guān)鍵因子以及不同海拔梯度土壤酶對(duì)溫度升高的響應(yīng),揭示未來(lái)氣候變暖對(duì)不同海拔梯度土壤有機(jī)質(zhì)分解的影響,得到如下結(jié)論：(1)土壤微生物產(chǎn)生的胞外酶可以降解土壤有機(jī)質(zhì)高分子物質(zhì)。本研究以北方溫帶森林老禿頂7個(gè)不同海拔梯度的土壤為研究對(duì)象,研究不同海拔梯度下土壤水解酶和氧化還原酶活性分布特征以及主要驅(qū)動(dòng)因素。研究結(jié)果表明：除N-乙酰-β-氨基葡萄糖苷酶(NAG)之外,α-萄糖苷酶(αG)、p-葡萄糖苷酶(pG)、纖維二糖水解酶(CHB)和木糖苷酶(pX)4種水解酶活性總體上隨著海拔的升高而增加,而土壤氧化還原酶活性并沒(méi)有表現(xiàn)出隨海拔梯度變化的規(guī)律。5種水解酶活性與有機(jī)碳(SOC)和顆粒有機(jī)碳(POC)含量呈顯著正相關(guān)(p0.05),與可溶性有機(jī)碳(DOC)含量呈極顯著正相關(guān)(p0.01)；除NAG以外,其它4種水解酶都與含水量(SWC)、全氮(TN)含量呈極顯著正相關(guān)；土壤過(guò)氧化物酶(PER)活性與SOC、TN和SWC含量呈極顯著正相關(guān),與POC顯著正相關(guān)；多酚氧化酶(PPO)只與土壤pH顯著正相關(guān)。這表明土壤水解酶活性與土壤有機(jī)質(zhì)的含量密切相關(guān)。(2)酶動(dòng)力學(xué)可以完整的描述酶催化反應(yīng)進(jìn)程,酶動(dòng)力學(xué)參數(shù)溫度敏感性可以反映其對(duì)溫度變化及未來(lái)氣候變暖情況下的響應(yīng)。本研究選擇北方溫帶森林老禿頂南坡3個(gè)不同海拔梯度,即1233 m(岳樺林)、1060 m(針闊混交林)、825 m(紅松林)森林土壤,進(jìn)行室內(nèi)不同溫度梯度培養(yǎng)試驗(yàn),研究土壤碳礦化速率和土壤pG動(dòng)力學(xué)參數(shù)及溫度敏感性。結(jié)果表明：溫度升高加快了不同海拔梯度土壤碳礦化速率(Cmin),且1233 m(岳樺林)處Cmin最高,海拔梯度和溫度對(duì)Cmin均有顯著影響(p0.001)碳礦化速率溫度敏感性CQ1O (Cmin))為1233 m(岳樺林)825 m(紅松林)1060 m(針闊混交林),但差異不顯著(p0.05)。土壤βG動(dòng)力學(xué)參數(shù)最大反應(yīng)速率(Vmax)和米氏常數(shù)(Km)均隨著培養(yǎng)溫度的升高而增加,Vmax的溫度敏感性(Q10(Vmax))變化范圍為1.78-1.90；Km的溫度敏感性(Q10(Km))變化范圍為1.79-2.0。1233 m(岳樺林)處Q10(Vmax)/Q10(Km)最高,顯著高于825 m(紅松林)和1060 m(針闊混交林),意味著高海拔岳樺林土壤有機(jī)質(zhì)碳分解受溫度升高影響最大。
[Abstract]:In this paper, the soil from 500-1306 m) on the south slope of old balding was studied under different vegetation types. The purpose of this study was to study the soil carbon mineralization rate through the soil enzyme activity of different elevation gradients. The kinetic parameters and temperature sensitivity of soil enzyme were studied, and the correlation analysis was used. The relationship between soil physical and chemical properties and soil physical and chemical properties was studied by means of redundancy analysis and principal component analysis. The key factors affecting soil enzyme changes at different elevations and the response of soil enzymes at different elevations to temperature rise were explored. Should. The effects of future climate warming on the decomposition of soil organic matter at different altitude gradient were revealed. Conclusion: (1) extracellular enzymes produced by soil microbes can degrade soil organic matter macromolecules. In this study, 7 soils with different elevation gradients in temperate forest in North China were studied. The distribution characteristics and main driving factors of soil hydrolase and redox enzyme activities at different elevation gradients were studied. The results showed that: except for N-acetyl- 尾 -glucosaminidase (NAG). The activities of 偽 -glucosidase (偽 -glucosidase) (偽 -glucosidase), cellulose hydrolase (CHB) and xylosidase (PX) increased with the increase of altitude. However, the activity of soil oxidoreductase did not show a significant positive correlation with the content of organic carbon (SOC) and particulate organic carbon (POC). There was a significant positive correlation with the content of soluble organic carbon (DOC). With the exception of NAG, the other four hydrolases were positively correlated with the contents of water content and total nitrogen. The activity of soil peroxidase peroxidase (per) was positively correlated with the contents of TN and SWC, and with POC. PPO was only positively correlated with soil pH, which indicated that soil hydrolase activity was closely related to the content of soil organic matter. The temperature sensitivity of enzyme kinetic parameters can reflect its response to temperature change and future climate warming. In this study, three different elevation gradients were selected for the southern slope of the old balding forest in the northern temperate zone. That is 1233m (Yuehuan forest) 1060m (coniferous and broad-leaved mixed forest) forest soil (Pinus koraiensis forest), the indoor temperature gradient culture experiment was carried out. The soil carbon mineralization rate, soil PG kinetic parameters and temperature sensitivity were studied. The results showed that the increase of temperature accelerated the soil carbon mineralization rate at different elevations. The Cmin was the highest at 1233m (birch forest). Elevation gradient and temperature had significant effects on Cmin (p0.001). The temperature sensitivity of CQ1O was 1233m (Pinus koraiensis forest) (Pinus koraiensis forest). 1060m (coniferous and broad-leaved mixed forest). But the difference was not significant (p0.05). The maximum reaction rate (Vmax.) and the Michlet constant (Km) of soil 尾 -G kinetic parameters increased with the increase of culture temperature. The temperature sensitivity of Vmax was 1.78-1.90. The range of temperature sensitivity of km is 1.79-2.0.1233 m (birch forest). Significantly higher than 825m (Pinus koraiensis) and 1060m (coniferous and broad-leaved mixed forest) means that soil organic matter carbon decomposition in high altitude birch forest is most affected by temperature rise.
【學(xué)位授予單位】：沈陽(yáng)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：S714

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