本文關鍵詞：應用PLFA技術分析氮沉降對三江平原小葉章濕地土壤微生物群落結構的影響　出處：《中南林業(yè)科技大學學報》2016年12期 　論文類型：期刊論文

  更多相關文章： 氮沉降 磷脂脂肪酸 小葉章濕地 微生物多樣性

【摘要】：為了研究氮沉降對三江濕地土壤微生物群落結構的影響,利用磷脂脂肪酸技術(PLFA)對三江平原小葉章濕地土壤微生物多樣性進行了分析。2010年5月,在黑龍江省科學院自然與生態(tài)研究所三江平原濕地生態(tài)定位研究站內(nèi)的小葉章濕地中建立模擬氮沉降試驗平臺,設置3個氮沉降濃度梯度:N1(0 g N·m-2a-1)、N2(4 g N·m-2a-1)、N3(8 g N·m-2a-1),于2014年6月用土鉆進行土壤樣品采集。結果表明:本研究共檢測到PLFAs 75種,其中特征脂肪酸29種。不同氮沉降處理下,真菌群落的生物量較高,土壤微生物生物量的總量介于30~33nmol·g-1。氮沉降增加對真菌群落數(shù)量的影響比較顯著(P0.05),但是對細菌和放線菌影響并不顯著。低氮時土壤微生物的數(shù)量最多,對照最低;高氮時土壤微生物群落的多樣性值和豐度值最高。根據(jù)典型性相關分析,得出銨態(tài)氮和硝態(tài)氮對土壤中真菌含量影響較為顯著。通過本研究可以得出,施氮增加了土壤微生物總量。銨態(tài)氮和硝態(tài)氮是影響土壤微生物數(shù)量的主要因子,低氮增加了土壤微生物多樣性,高氮則產(chǎn)生抑制作用。
[Abstract]:In order to study the effect of nitrogen deposition on soil microbial community structure in Sanjiang wetland. The microbial diversity in the soil of the small leaf chapter wetland in Sanjiang Plain was analyzed by using phospholipid fatty acid technique (PLFAA). May 2010. An experimental platform for simulating nitrogen deposition was established in the wetland ecological location station of Sanjiang Plain of Heilongjiang Academy of Sciences. Three nitrogen deposition gradient of N _ (1) 0 g N 路m ~ (-2) a ~ (-1) N ~ (2 +) ~ (4 g N 路m ~ (-2) a ~ (-1) N _ 3N _ (3) N 路m ~ (-2) a ~ (-1)). In June 2014, soil samples were collected with soil drill. The results showed that 75 species of PLFAs were detected in this study, including 29 species of characteristic fatty acids. The biomass of fungi community was higher, and the total biomass of soil microorganism was between 30 ~ 33nmol 路g ~ (-1). The effect of nitrogen deposition on the quantity of fungi community was significant (P0.05). But the effect on bacteria and actinomycetes was not significant. The diversity and abundance of soil microbial communities were the highest when nitrogen was high. According to the typical correlation analysis, the effects of ammonium nitrogen and nitrate nitrogen on the soil fungal content were significant. Ammonium nitrogen and nitrate nitrogen were the main factors affecting soil microbial quantity, low nitrogen increased soil microbial diversity, and high nitrogen had inhibitory effect.
【作者單位】： 東北林業(yè)大學林學院;黑龍江省科學院自然與生態(tài)研究所濕地與生態(tài)保育國家地方聯(lián)合工程實驗室;
【基金】：國家自然科學基金項目(31470019,31400429,31500410) 十三五國家重點研發(fā)計劃(2016YFC0500405) 黑龍江省科學院項目(STJB16-01,STJB16-04,2015YZ01,2014ST05) 黑龍江省博士后基金(LBH-Z14184) 黑龍江省科研機構創(chuàng)新能力提升專項計劃(GY2014KC0039) 黑龍江省科學院青年創(chuàng)新基金資助
【分類號】：S154.3
【正文快照】： (1.College of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China;2.National and Provincial Joint EngineeringLaboratory of Wetlands and Ecological Conservation Institute of NatureEcology,Heilongjiang Academy of Sciences,Harbin 150040

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