【摘要】：為分析玉米ZmPGP1基因啟動子的功能,利用巢式PCR方法克隆出了玉米ZmPGP1基因的啟動子調(diào)控區(qū),并將該啟動子與GUS基因融合,通過基因槍法轉(zhuǎn)入玉米(Zea mays)中,分析ZmPGP1啟動子表達特性。結(jié)果顯示,在玉米中克隆出ZmPGP1基因5′端上游1 090bp的啟動子序列,該啟動子序列包括光響應元件、激素響應元件和脅迫誘導及發(fā)育相關(guān)順式作用元件。GUS染色表明ZmPGP1基因在玉米幼苗的莖部、葉子及根中都有表達,其中莖的節(jié)間處以及葉鞘部位表達量較高,這與ZmPGP1基因的Real-time PCR分析結(jié)果一致。研究結(jié)果進一步闡明ZmPGP1基因的功能以及作用機理。
[Abstract]:In order to analyze the function of the maize ZmPGP1 gene promoter, the promoter regulatory region of the maize ZmPGP1 gene was cloned by a nested PCR method, and the promoter was fused with the GUS gene. The expression of the ZmPGP1 promoter was analyzed by the gene gun method. The results showed that the promoter sequence of 1 090 bp upstream of the 5-terminal end of the ZmPGP1 gene was cloned in the maize, and the promoter sequence included light response element, hormone response element and stress-inducing and developing-related cis-acting element. The GUS staining showed that the ZmPGP1 gene was expressed in the stem, leaf and root of the maize seedling, and the expression of the internode of the stem and the leaf-leaf part was high, which was consistent with the results of the Real-time PCR analysis of the ZmPGP1 gene. The results of the study further illustrate the function and mechanism of the ZmPGP1 gene.
【作者單位】： 江蘇省農(nóng)墾農(nóng)業(yè)科學研究院;中國農(nóng)業(yè)大學農(nóng)學院;
【基金】：國家自然科學基金項目(31171564) 公益性行業(yè)(農(nóng)業(yè))科技專項(201303002)
【分類號】：S513
，

本文編號：2503273