【摘要】：羥基磷灰石是脊椎動物的牙齒和骨骼的主要成分,良好的生物相容性,高度的成骨潛能性,可以通過骨傳導(dǎo)機制促進新骨的生長而不會產(chǎn)生任何局部或全身的炎癥反應(yīng),也可以作為模板化合物,用于研究人體中的生物礦化。HAp粒子還可以抑制多種癌細(xì)胞的生長,是作為生物醫(yī)學(xué)應(yīng)用的首選材料。通過控制HAp晶體的形貌、尺寸和粒徑分布,可以擴大它的潛在應(yīng)用。本論文以Ca(NO_3)_2·4H_2O和(NH_4)_3PO_4·3H_2O為原料,以Gemini陽離子型表面活性劑為模板,采用生物礦化法和水熱法,通過改變不同的實驗條件制備出具有特殊形貌的HAp。選取不同比表面積和孔徑的HAp樣品為載體,以萬古霉素為模型,通過改變藥物負(fù)載條件,研究藥物的負(fù)載性能和緩釋能力。采用X射線衍射儀(XRD)、傅立葉變換紅外光譜(FT-IR)、掃描電鏡(SEM)、熱重分析(TG)分別對產(chǎn)物的晶相結(jié)構(gòu)、組成、晶體形貌、熱學(xué)性能進行分析,并分析了不同形貌的HAp晶體形成的機理。實驗表明,Gemini陽離子表面活性劑的濃度對HAp形貌產(chǎn)生影響,有機模板的極性基團和HAp晶體間的相互作用能夠影響HAp的成核和生長。用紫外可見分光光度計(UV)測定樣品的吸光度,分析藥物的負(fù)載能力和緩釋性能。實驗表明在37 ℃的負(fù)載溫度下,載藥時間為24h時,HAp的比表面積越大,萬古霉素的負(fù)載濃度越大,更有利于藥物的負(fù)載。最佳負(fù)載條件下,藥物負(fù)載容量為78.95%,藥物負(fù)載量為44.12 mg/g,包封率為58.83%,累積釋放率為84%。
[Abstract]:Hydroxyapatite is a major component of the teeth and bones of vertebrates, with good biocompatibility, high osteogenic potential, and the ability to promote the growth of new bones through bone conduction without any local or systemic inflammatory response. HAp particles can also inhibit the growth of many kinds of cancer cells and are the preferred materials for biomedical applications. The potential application of HAp crystal can be expanded by controlling its morphology, size and particle size distribution. In this paper, Ca (NO_3) _ 2 4H_2O and (NH_4) _ 3PO_4 3H_2O were used as raw materials and Gemini cationic surfactant as template. HAp. with special morphology was prepared by changing different experimental conditions. HAp samples with different specific surface area and pore size were selected as carriers, vancomycin was used as model, and the loading performance and sustained release ability of vancomycin were studied by changing drug loading conditions. X-ray diffraction (XRD) (XRD), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscope (SEM),) thermogravimetric analysis (TG) were used to analyze the crystal phase structure, composition, crystal morphology and thermal properties of the products, respectively. The formation mechanism of HAp crystals with different morphologies was analyzed. The experimental results show that the concentration of Gemini cationic surfactants affects the morphology of HAp, and the interaction between the polar groups of organic templates and HAp crystals can affect the nucleation and growth of HAp. The absorbance of the sample was determined by UV-vis spectrophotometer (UV), and the drug loading capacity and slow-release property were analyzed. The experimental results show that the larger the specific surface area of HAp is, the greater the loading concentration of vancomycin is, and the better the loading time is when the loading temperature is 37 鈩，

本文編號：2380333