【摘要】：磁分離技術(shù)已經(jīng)有很多年的發(fā)展歷史,因分離方法簡便快捷、目標性強而廣泛應用在生物分離、廢水處理等領(lǐng)域中。但是由于該技術(shù)難以實現(xiàn)放大和連續(xù)化,目前在工業(yè)中還尚未推廣應用。本文通過將浮選技術(shù)耦合到磁分離技術(shù)中,發(fā)展了新型氣助磁分離技術(shù)。以3.3μm的磁性聚甲基丙烯酸縮水甘油酯微球(MPNs)用于牛血清白蛋白BSA的分離作為模擬體系,開展了間歇、連續(xù)和全流程連續(xù)氣助磁分離過程的研究,研究內(nèi)容主要包括下列幾個方面:1)采用分散聚合法制備磁性聚甲基丙烯酸縮水甘油酯微球,在微球表面修飾親和配基,以實現(xiàn)目標蛋白的親和吸附分離�？疾炝说鞍踪|(zhì)的初始濃度、溶液pH、離子強度等因素對蛋白吸附性能的影響,獲得蛋白質(zhì)的最佳吸附條件。2)在磁性載體的最佳吸附條件下,考察并優(yōu)化了萃取、洗滌、反萃個單元過程的主要工藝參數(shù)�？疾炝巳芤旱膒H值、氣體流速、裝載體積等因素對分離效率的影響,對于含磁顆粒濃度為0.72 mg/mL的料液,在pH值為7.2的條件下,裝載體積為250 mL,氣體流速為60 mL/min,其分離速率為410 mL/min,回收率達98%。其結(jié)果表明:間歇條件下,氣速能大大加速磁分離過程且能實現(xiàn)磁顆粒的遠距離捕獲。與單純的磁分離相比,其對磁場的依賴性大為降低,并且分離過程易于放大。同時也證明該分離過程不會導致蛋白質(zhì)從磁顆粒表面脫落,也不會引起蛋白質(zhì)構(gòu)象的變化。3)在此基礎(chǔ)上,進一步開展了連續(xù)氣助磁分離過程的研究,當氣速為250mL/min時,其處理量達24 L/h,95%以上的磁顆粒從上部磁輥流出,底部流出液中磁顆粒的濃度僅為0.0389 mg/m L,整個過程質(zhì)量基本守恒。該過程順利實現(xiàn)了生物分離過程中磁性微球的規(guī)模化連續(xù)分離。4)基于上述各單元過程的研究,將全流程規(guī)�；B續(xù)氣助磁分離中試裝置用于蛋白質(zhì)萃取、洗滌和反萃過程的連續(xù)化分離,證明了該過程的可行性和高效性。
[Abstract]:Magnetic separation technology has been developed for many years. It has been widely used in biological separation, wastewater treatment and other fields because of its simple and fast separation method and strong target. However, because the technology is difficult to realize amplification and continuity, it has not yet been popularized and applied in industry. In this paper, a new gas-assisted magnetic separation technology is developed by coupling flotation technology with magnetic separation technology. Using 3.3 渭 m magnetic polyglycidyl methacrylate microspheres (MPNs) as a simulation system for the separation of bovine serum albumin (BSA) BSA, the intermittent, continuous and whole-process continuous gas-assisted magnetic separation processes were studied. The main contents of this study are as follows: 1) Magnetic polyglycidyl methacrylate microspheres were prepared by dispersion polymerization. The affinity ligands were modified on the surface of the microspheres to achieve the affinity adsorption separation of the target proteins. The effects of the initial concentration of protein, pH of solution and ionic strength on the adsorption properties of protein were investigated. The optimum adsorption conditions of protein were obtained. 2) under the optimum adsorption conditions of magnetic carrier, the extraction and washing of protein were investigated and optimized. The main process parameters of the stripping unit process. The effects of pH value of solution, gas flow rate and loading volume on the separation efficiency were investigated. When the concentration of magnetic particles was 0. 72 mg/mL, the pH value was 7. 2. The loading volume is 250 mL, the gas flow rate is 60 mL / min, the separation rate is 410 mL / min, and the recovery is 98%. The results show that gas velocity can greatly accelerate the magnetic separation process and realize the long distance capture of magnetic particles under intermittent conditions. Compared with simple magnetic separation, its dependence on magnetic field is greatly reduced, and the separation process is easy to amplify. It is also proved that the separation process will not cause protein to fall off the surface of magnetic particles or cause the conformation of protein to change. 3) on this basis, the continuous gas-assisted magnetic separation process has been further studied. When the gas velocity is 250mL/min, More than 95% of the magnetic particles were disposed of from the upper magnetic roller, and the concentration of the magnetic particles in the bottom effluents was only 0.0389 mg/m / L. the quality of the whole process was basically conserved. This process successfully realized the large-scale continuous separation of magnetic microspheres in biological separation. Based on the research of each unit process mentioned above, the whole process of large-scale continuous gas-assisted magnetic separation (GASM) was applied to the extraction of protein. The continuous separation of washing and stripping process proves the feasibility and efficiency of the process.
【學位授予單位】：湘潭大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TQ028;O629.73

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本文編號：2172517