發(fā)布時間：2018-06-29 23:42

  本文選題：紙芯片 + 回形針　； 參考：《煙臺大學(xué)》2017年碩士論文

【摘要】：微流控紙芯片是一種新型的簡易分析設(shè)備,它將微流控技術(shù)的微型化與紙質(zhì)材料的廉價易得等優(yōu)點相結(jié)合,發(fā)展成為當(dāng)前備受關(guān)注的熱點領(lǐng)域,在環(huán)境監(jiān)測,食品質(zhì)量控制以及生物醫(yī)學(xué)等方面都呈現(xiàn)出巨大的應(yīng)用潛力。本文主要研究一種新型的3D紙芯片制作方法,同時設(shè)計了兩種流體控制方式,并對制作的紙芯片進行了功能化研究。本論文可以分為兩個部分:第一部分為綜述。首先,介紹了紙芯片的優(yōu)點,加工、檢測方法以及應(yīng)用等;其次,介紹了3D紙芯片的研究進展,重點突出了其加工方法及流體控制方法;最后,闡述了酶聯(lián)免疫反應(yīng)在紙芯片方面的具體應(yīng)用。第二部分為研究報告,主要由三章構(gòu)成,具體內(nèi)容如下:第一章,建立一種新型的3D微流控紙芯片制作技術(shù)-回形針固定法。設(shè)計多種不同紙芯片,并對回形針方法的重現(xiàn)性、紙芯片的通道及性能進行考察。利用自主研制的紙芯片比色檢測裝置,對牛血清白蛋白(BSA)、二價鐵離子進行定量檢測。在所規(guī)定的范圍內(nèi),都獲得了較好的線性關(guān)系。同時,我們將回形針固定法與其他幾種方法進行了比較,證明回形針固定法的可行性。第二章,基于前面回形針固定法的基礎(chǔ)上,設(shè)計一種新的控制流體流動的方法-移動閥,并實現(xiàn)了對癌癥標(biāo)志物癌胚抗原(CEA)的比色免疫檢測。首先,利用蠟印法制作紙芯片,并將紙芯片和移動閥相結(jié)合,制作了多種形式的移動閥紙芯片,介紹了其工作原理并對其性能進行探究;其次,將比色酶聯(lián)免疫分析與移動閥紙芯片相結(jié)合,構(gòu)建了微流控紙芯片比色免疫傳感器,根據(jù)顯色信號的強度,對CEA的濃度進行定量分析。同時,利用這種方法檢測了實際血清中的CEA,驗證移動閥紙芯片的可行性。第三章,主要研究一種新的圓形的旋轉(zhuǎn)閥紙芯片,并將紙芯片與辣根過氧化物酶(HRP)催化的魯米諾化學(xué)發(fā)光免疫體系結(jié)合,建立一種在紙芯片上檢測血清中癌癥標(biāo)識物甲胎蛋白(AFP)的新方法,同時搭建了一種簡單的化學(xué)發(fā)光檢測設(shè)備�；贏FP上標(biāo)記的HRP能有效催化luminol-H2O2化學(xué)發(fā)光體系的化學(xué)發(fā)光,根據(jù)化學(xué)發(fā)光信號強度與AFP濃度存在的關(guān)系,實現(xiàn)對甲胎蛋白的即時檢測。結(jié)果表明,二者呈現(xiàn)良好的線性關(guān)系,同時,利用此方法檢測了實際血清中的AFP,證明了旋轉(zhuǎn)閥紙芯片的可操作性。
[Abstract]:Microfluidic paper chip is a new type of simple analysis equipment. It combines the advantages of microfluidic technology and cheap and easy to obtain paper materials. Food quality control and biomedicine have great application potential. In this paper, a new method of making 3D paper chip is studied, two kinds of fluid control methods are designed, and the function of the paper chip is studied. This paper can be divided into two parts: the first part is a summary. Firstly, the advantages, processing, testing methods and applications of paper chip are introduced. Secondly, the research progress of 3D paper chip is introduced, especially its machining method and fluid control method. The application of enzyme-linked immunoreactivity (Elisa) in paper chip was described. The second part is the research report, which consists of three chapters. The main contents are as follows: in chapter 1, a new 3D microfluidic paper chip fabrication technique, paper clip fixing method, is established. Several different paper chips were designed, and the reproducibility of paper clip method, the channel and performance of paper chip were investigated. Bovine serum albumin (BSA) and divalent iron ions were quantitatively detected by a paper chip colorimetry device developed by ourselves. A good linear relationship is obtained within the prescribed range. At the same time, the paper clip fixation method is compared with other methods to prove the feasibility of paper clip fixation method. In the second chapter, a new method to control the flow of fluid, the moving valve, is designed, and the colorimetric detection of carcinoembryonic antigen (CEA), a cancer marker, is realized. Firstly, the paper chip is made by wax printing method, and the paper chip and the moving valve are combined to make various kinds of moving valve paper chip. The working principle of the chip is introduced and its performance is explored. A microfluidic paper chip colorimetric immunosensor was constructed by combining colorimetric enzyme linked immunoassay (Elisa) with moving valve paper chip. The concentration of CEA was quantitatively analyzed according to the intensity of color signal. At the same time, this method was used to detect the CEA in real serum to verify the feasibility of mobile valve paper chip. In chapter 3, a new circular rotary valve paper chip was studied, and the paper chip was combined with the luminol chemiluminescence immune system catalyzed by horseradish peroxidase (HRP). A new method for the detection of alpha-fetoprotein (AFP) in serum on paper chip was established, and a simple chemiluminescence detection equipment was built. HRP labeled with AFP can effectively catalyze the chemiluminescence of luminol-H2O2 chemiluminescence system. According to the relationship between the intensity of chemiluminescence signal and the concentration of AFP, the real-time detection of alpha-fetoprotein can be realized. The results show that there is a good linear relationship between them. Meanwhile, AFP in serum is detected by this method, which proves the maneuverability of rotary valve paper chip.
【學(xué)位授予單位】：煙臺大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TQ056.16

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