本文選題：生物制氫 + 混合菌��； 參考：《北京化工大學(xué)》2015年碩士論文

【摘要】：在當(dāng)前氣候和能源危機的時代,高熱值、清潔能源氫氣被認(rèn)為是最理想的化石能源的替代品。厭氧發(fā)酵技術(shù)操作條件溫和,可以以固體廢棄物或廢水中的有機生物質(zhì)為底物,在發(fā)酵產(chǎn)氫的同時緩解環(huán)境污染問題,成為氫能研究領(lǐng)域的熱點。但是傳統(tǒng)的懸浮發(fā)酵系統(tǒng)存在菌體易被洗出、產(chǎn)氫效率低的缺點。針對以上問題,本論文對從活性污泥中篩選出的兩株高效產(chǎn)氫菌進行了共固定化的研究。首先對比了纖維材料、活性炭和生物質(zhì)吸附材料三種吸附載體對復(fù)合菌的固定化效果,得到最佳吸附載體為生物質(zhì)吸附材料,反復(fù)10個批次發(fā)酵的平均產(chǎn)氫量為1845 mL,平均產(chǎn)氫得率為1.50 mol H2/mol glucose,比游離發(fā)酵提高了62.5%。并且固定化提高了菌體對底物的耐受性,淀粉酶的活性比懸浮發(fā)酵提高了2-3倍,己糖激酶的活性比懸浮發(fā)酵提高了2倍左右。在以上基礎(chǔ)上,還考察了固定化復(fù)合菌對木糖的利用情況。結(jié)果表明,固定化復(fù)合菌能快速利用木糖,最大產(chǎn)氫速度為懸浮發(fā)酵的4.5倍。反復(fù)10個批次發(fā)酵的平均底物利用率為96%,平均產(chǎn)氫量為1972 mL,平均產(chǎn)氫得率為1.47 mol H2/mol xylose,為復(fù)合菌以纖維素水解液產(chǎn)氫提供了依據(jù)。本論文將從活性污泥中篩選出的兩株產(chǎn)氫菌進行共固定化,在保持兩株菌協(xié)同高效產(chǎn)氫的同時,加快了產(chǎn)氫速度,驗證了固定化復(fù)合菌反復(fù)實驗的可行性,為固定化連續(xù)發(fā)酵產(chǎn)氫奠定了基礎(chǔ)。
[Abstract]:In the current climate and energy crisis era, high calorific value, clean energy hydrogen is considered as the ideal alternative to fossil energy. Anaerobic fermentation technology has mild operating conditions and can be used as substrate of solid waste or organic biomass in wastewater to reduce environmental pollution while producing hydrogen by fermentation. It has become a hot spot in the field of hydrogen energy research. However, the traditional suspension fermentation system has the disadvantages of easy washing out of bacteria and low hydrogen production efficiency. In order to solve the above problems, the co-immobilization of two high efficient hydrogen producing bacteria from activated sludge was carried out in this paper. Firstly, the immobilization effect of fiber material, activated carbon and biomass adsorption material on composite bacteria was compared, and the best adsorption carrier was biomass adsorption material. The average hydrogen production was 1845 mL and the average hydrogen yield was 1.50 mol H2/mol glucose, which was 62.5% higher than that of free fermentation. The immobilization improved the substrate tolerance, the activity of amylase was 2-3 times higher than that of suspension fermentation, and the activity of hexokinase was about 2 times higher than that of suspension fermentation. On the basis of above, the utilization of xylose by immobilized compound bacteria was also investigated. The results showed that the immobilized compound bacteria could rapidly utilize xylose and the maximum hydrogen production rate was 4.5 times of that of suspension fermentation. The average substrate utilization ratio was 96%, the average hydrogen production was 1972 mL and the average hydrogen production rate was 1.47 mol H2/mol xylose. it provided the basis for the compound bacteria to produce hydrogen by cellulose hydrolysate. In this paper, two strains of hydrogen producing bacteria selected from activated sludge were co-immobilized. While keeping the two strains producing hydrogen synergistically and efficiently, the rate of hydrogen production was accelerated, and the feasibility of repeated experiments of immobilized compound bacteria was verified. It lays a foundation for continuous fermentation hydrogen production by immobilized.
【學(xué)位授予單位】：北京化工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TQ929;TQ116.2

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相關(guān)期刊論文 前1條

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本文編號：1946776