本文選題：MFC + 生物產(chǎn)電�。� 參考：《中國環(huán)境科學(xué)》2017年09期

【摘要】：采用厭氧雙層平板法從運行穩(wěn)定的雙室型微生物燃料電池(Microbial fuel cell,MFC)陽極生物膜上分離純化出一株優(yōu)勢產(chǎn)電純菌Klebsiella oxytoca,并對其產(chǎn)電機理進行了研究.通過對該純菌的循環(huán)伏安掃描分析,表明該菌具有較強的電化學(xué)活性,并且在胞外存在電子受體時就會進行胞外產(chǎn)電呼吸.對該菌培養(yǎng)物進行三維熒光掃描和比較,發(fā)現(xiàn)該菌主要是利用腐殖質(zhì)作為胞外電子傳遞的介體來完成產(chǎn)電呼吸.鐵還原性測試的結(jié)果表明過量的3價鐵沒有給菌株帶來額外的增長,該菌對3價鐵的還原量有限.研究認為,胞外呼吸不是Klebsiella oxytoca在無氧條件下的主要呼吸方式,而是當有外在電子受體存在時細菌進行胞內(nèi)呼吸的一種輔助方式,即使環(huán)境中存在充分的胞外電子受體,也不會帶來Klebsiella oxytoca生長量的顯著增加,該菌無法從胞外還原中獲得足夠的生長需要的能量,仍然主要是通過胞內(nèi)呼吸獲得細胞生長所需的能量,有機物氧化所產(chǎn)生的電子只有少部分用于胞外電子受體的還原.
[Abstract]:A dominant electricity-producing bacterium Klebsiella oxytoca was isolated and purified from the anodic biofilm of biologically stable biofilm of biofilm, Klebsiella oxytoca, and its motor mechanism was studied. The results of cyclic voltammetry showed that the pure strain had strong electrochemical activity and the extracellular electrorespiration occurred when there was an electron receptor outside the cell. Three-dimensional fluorescence scanning and comparison showed that humus was mainly used as the mediator of extracellular electron transfer to complete electrorespiration. The results of iron reductivity test showed that excessive trivalent iron did not increase the strain and that the strain had limited reduction of trivalent iron. It is believed that extracellular respiration is not the main breathing mode of Klebsiella oxytoca under anaerobic conditions, but an auxiliary way for bacteria to breathe in the presence of external electron receptors, even if there are sufficient extracellular electron receptors in the environment. Nor does it lead to a significant increase in Klebsiella oxytoca growth. The bacteria can't get enough energy from extracellular reduction to grow. It still gets the energy it needs to grow mainly through intracellular respiration. Only a few of the electrons produced by organic oxidation are used for the reduction of extracellular electron receptors.
【作者單位】： 西安建筑科技大學(xué)陜西省環(huán)境工程重點實驗室教育部西北水資源和環(huán)境生態(tài)重點實驗室;
【基金】：重大水專項(2009ZX07212-002)
【分類號】：TM911.45

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1 ;Enhanced hydrogen production by insertional inactivation of adhE gene in Klebsiella oxytoca HP1[J];Chinese Science Bulletin;2007年04期

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本文編號：1906979