發(fā)布時間：2018-04-26 03:36

  本文選題：費(fèi)氏丙酸桿菌 + 丙酸��； 參考：《河北大學(xué)》2017年碩士論文

【摘要】：微生物發(fā)酵法生產(chǎn)丙酸受到越來越多的關(guān)注,但是人們的焦點(diǎn)主要聚集在如何解除產(chǎn)物反饋抑制、提高底物轉(zhuǎn)化率等方面,對發(fā)酵液中丙酸的分離提取卻鮮有關(guān)注。本論文研究和考察了ZGD630陰離子交換樹脂吸附-解吸費(fèi)氏丙酸桿菌發(fā)酵液中丙酸和丁二酸的工藝參數(shù),建立了“兩段式”層析法聯(lián)產(chǎn)發(fā)酵液中丙酸和丁二酸的提取工藝,并完成了10L層析柱的放大試驗(yàn),為丙酸和丁二酸的半連續(xù)耦合發(fā)酵奠定了基礎(chǔ)。本論文主要取得了如下研究成果:(1)研究和考察了ZGD630陰離子交換樹脂吸附分離費(fèi)氏丙酸桿菌發(fā)酵液中丙酸等代謝產(chǎn)物的最佳吸附條件。結(jié)果表明,采用001×7陽離子交換樹脂對發(fā)酵上清液進(jìn)行酸化處理后,pH可降至1.5。此時,ZGD630陰離子交換樹脂對丙酸和丁二酸的靜態(tài)吸附載量分別為89.4 mg/g和108.2 mg/g,較初始發(fā)酵液(pH 6.8)的吸附載量分別提高了101.35%和71.74%。(2)考察了ZGD630陰離子交換樹脂對酸化發(fā)酵液中丙酸和丁二酸的動態(tài)吸附特性。結(jié)果表明,酸化后的發(fā)酵液過量上樣至裝填有ZGD630陰離子交換樹脂的層析柱時,丁二酸會優(yōu)先被ZGD630陰離子交換樹脂吸附,當(dāng)發(fā)酵液中丁二酸被完全吸附后,丙酸才開始被吸附。此時,穿柱液按體積可分為三部分:0-2 BV,不含丁二酸、丙酸;2-6.5BV,不含丁二酸,含丙酸;6.5 BV,含丁二酸、丙酸三個部分(BV均指樹脂體積)。(3)研究建立了“兩段式”層析法聯(lián)產(chǎn)丙酸和丁二酸的工藝,并確定了其操作參數(shù)。結(jié)果表明,首先采用層析柱Ⅰ吸附發(fā)酵液中的丁二酸,最佳的上樣流速為3 BV/h,上樣體積為10 BV,丁二酸可完全穿柱;然后將層析柱Ⅰ的穿柱液(不含丁二酸的部分)上樣至層析柱Ⅱ,上樣流速為3 BV/h,上樣體積為5 BV,以確保其吸附飽和。(4)層析柱Ⅰ采用NaOH溶液進(jìn)行洗脫,最適的NaOH溶液洗脫濃度為3 mol/L,洗脫流速0.45 BV/h,丁二酸最高濃度可達(dá)91.28 g/L,是發(fā)酵液中丁二酸濃度的7.1倍。層析柱Ⅱ采用H2SO4溶液進(jìn)行洗脫,結(jié)果表明,最適的H2SO4溶液洗脫濃度為4 mol/L,洗脫流速0.6 BV/h,丙酸最高濃度可達(dá)97.02 g/L,是發(fā)酵液中丙酸濃度的4.4倍。(5)對上述工藝進(jìn)行10 L層析柱的中試放大試驗(yàn),取得了良好的效果。丙酸和丁二酸的回收率分別達(dá)到了88.12%和91.05%。
[Abstract]:More and more attention has been paid to the production of propionic acid by microbial fermentation, but the focus is mainly on how to remove the product feedback inhibition and improve the substrate conversion, but little attention has been paid to the separation and extraction of propionic acid from fermentation broth. In this paper, the process parameters of ZGD630 anion exchange resin for adsorption and desorption of propionic acid and succinic acid in the fermentation broth of Propionibacterium fermentum were studied, and the extraction process of propionic acid and succinic acid in the fermentation broth by "two-stage" chromatography was established. The amplification experiment of 10 L chromatographic column was completed, which laid a foundation for semi-continuous coupling fermentation of propionic acid and succinic acid. In this paper, the following research results were obtained: 1) the optimal adsorption conditions for the adsorption and separation of propionic acid and other metabolites from the fermentation broth of Propionibacterium fermentum by ZGD630 anion exchange resin were investigated. The results showed that the pH of ferment supernatant could be reduced to 1.5 after acidizing with 001 脳 7 cation exchange resin. The static adsorption capacity for propionic acid and succinic acid by ZGD630 anion exchange resin was 89.4 mg/g and 108.2 mg / g respectively, which was 101.35% and 71.74% higher than that of initial fermentation broth (pH 6.8). The effect of ZGD630 anion exchange resin on acidizing fermentation broth was investigated. Dynamic adsorption characteristics of propionic acid and succinic acid. The results showed that when the acidified fermentation broth was overloaded with ZGD630 anion exchange resin, the succinic acid would be preferentially adsorbed by ZGD630 anion exchange resin, and when the succinic acid in the fermentation broth was completely adsorbed, propionic acid would begin to be adsorbed. At this time, the liquid can be divided into three parts according to volume: 0-2 BV, no succinic acid, 2. 5 BV propionate, no succinic acid, 6. 5 BV propionic acid, and 5. 5 BV succinic acid. BV of three parts of propionic acid refers to the volume of resin. The process of producing propionic acid and succinic acid by "two-stage" chromatography has been studied and its operating parameters have been determined. The results showed that the best flow rate was 3 BV / h and the volume of the sample was 10 BV, and succinic acid could penetrate the column completely. Then the column I was eluted with NaOH solution at a flow rate of 3 BV / h and a volume of 5 BV in order to ensure its adsorption saturation. The optimal elution concentration of NaOH solution is 3 mol / L, the elution rate is 0.45 BV / h, and the highest concentration of succinic acid can reach 91.28 g / L, which is 7.1 times the concentration of succinic acid in fermentation broth. Chromatographic column 鈪，

本文編號：1804350