本文選題：親和膜 + 溶液噴射紡絲技術　； 參考：《天津工業(yè)大學》2017年碩士論文

【摘要】：親和膜分離是兼具膜分離和親和色譜技術的新型生物純化技術,能實現(xiàn)特異性和高純化率吸附。隨著納米纖維制備技術的發(fā)展,納米纖維親和膜技術受到了廣泛關注。殼聚糖作為天然的吸附劑,表現(xiàn)出良好的吸附性能,本研究采用殼聚糖衍生物作為親和膜吸附材料,利用溶液噴射紡絲技術制備納米纖維親和膜,將為蛋白質的特異性吸附分離研究提供新思路和方法。本論文首先利用1-(3-二甲氨基丙基)-3-乙基碳二亞胺(EDC)和N-羥基琥珀酰亞胺(NHS)作為催化交聯(lián)劑,制備了精氨酸/殼聚糖一系列不同取代度的精氨酸殼聚糖(CS-Arg),利用FTIR、13CNMR、XRD、TG和EA等對其結構表征,結果表明隨著精氨酸的量增加,取代度呈現(xiàn)出先增大后減小的趨勢。論文進一步利用制得的CS-Arg與PLA作為混紡溶液,通過溶液噴射紡絲技術制備納米纖維(CANFs),通過SEM、接觸角、過濾效率及阻力、FTIR和XPS等來表征納米纖維親和膜,結果表明,隨著取代度的增加,纖維直徑稍微變大,平均直徑在100～200nm,CANFs膜具有親水性且有高的過濾效率和低的過濾阻力。將其應用于親和吸附,對CANFs親和膜的吸附分離牛血清蛋白性能進行研究,結果表明親和膜的吸附能力良好,最大吸附量達445.19mg/g;探討了 pH值、初始濃度、吸附時間對BSA的吸附性能的影響,最佳吸附條件為pH=7.14,吸附時間為10h和初始濃度為3.0mg/mL。對CANFs親和膜吸附BSA的吸附等溫線模型、動力學模型和分子擴散模型研究可知,CANFs親和膜吸附BSA符合Langmuir模型,屬于單分子層吸附,且對BSA的親和作用在中等范圍內,為有效吸附,容易洗脫;吸附速率符合準二級動力學模型,不符合分子擴散模型,分子擴散速率控制步驟為化學吸附作用。CANFs親和膜對BSA的洗脫研究可知,有很高的再生吸附性能,可以重復使用,穩(wěn)定性好。本論文通過制備納米纖維親和膜介質,研究了其吸附性能和吸附模型,這為新型氨基酸配基的開發(fā)、親和色譜分離過程研究提供相關數(shù)據(jù)和理論指導,從而拓寬了親和膜技術的應用領域。
[Abstract]:Affinity membrane separation is a new biological purification technology with both membrane separation and affinity chromatography. With the development of nanofiber preparation technology, nanofiber affinity membrane technology has received extensive attention. Chitosan, as a natural adsorbent, has good adsorption properties. In this study, chitosan derivatives were used as affinity membrane adsorbents, and nano-fiber affinity membranes were prepared by solution jet spinning technique. It will provide a new idea and method for the study of protein specific adsorption and separation. In this paper, 1-HYDIMETHYLSANIMIDINE (1-HYDROXYSucinimide) and N-hydroxy-succinimide (NHS) were first used as cross-linking agents. A series of arginine chitosan (CS-Arg) with different degree of substitution were prepared and characterized by FTIR13CNMR-XRDX TG and EA. The results showed that the degree of substitution increased first and then decreased with the increase of arginine content. In this paper, CS-Arg and PLA were further used as blending solution to prepare nanofibers CANFS by solution jet spinning technology. The affinity membranes were characterized by SEM, contact angle, filtration efficiency and resistance, FTIR and XPS. With the increase of the degree of substitution, the fiber diameter increased slightly, and the average diameter of CANFs membrane was 100 ~ 200nm ~ (-1). The membrane had high hydrophilicity, high filtration efficiency and low filtration resistance. The adsorption and separation of bovine serum protein by CANFs affinity membrane were studied. The results showed that the adsorption capacity of affinity membrane was good, the maximum adsorption capacity was 445.19 mg / g, the pH value and initial concentration of bovine serum protein were discussed. The effect of adsorption time on the adsorption performance of BSA was investigated. The optimum adsorption conditions were pH = 7.14, adsorption time 10 h and initial concentration 3.0 mg 路mL ~ (-1). The adsorption isotherm model, kinetic model and molecular diffusion model for the adsorption of BSA by CANFs affinity membrane showed that the adsorption of BSA by CANFs membrane was in accordance with the Langmuir model and belonged to monolayer adsorption, and the affinity to BSA was in a medium range, which was an effective adsorption. The adsorption rate accords with the quasi-second-order kinetic model, and does not accord with the molecular diffusion model. The control step of molecular diffusion rate is chemisorption. The elution of BSA by CANFs affinity membrane shows that it has high regenerative adsorption performance. Can be reused, good stability. In this paper, the adsorption properties and adsorption models of nanofibers were studied by preparing affinity membrane media, which provided relevant data and theoretical guidance for the development of novel amino acid ligands and the separation process of affinity chromatography. Thus, the application field of affinity membrane technology is widened.
【學位授予單位】：天津工業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：TQ051.893

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