發(fā)布時(shí)間：2018-03-31 19:00

  本文選題：瓜果類生物質(zhì)　切入點(diǎn)：光合細(xì)菌　出處：《河南農(nóng)業(yè)大學(xué)》2015年碩士論文

【摘要】：中國瓜果生產(chǎn)量遙居世界首位,其中具有代表性的蘋果和西瓜年產(chǎn)量分別達(dá)到2千萬噸和7千萬噸。巨大數(shù)量的水果產(chǎn)量勢必會因?yàn)閻毫犹鞖狻㈤L途運(yùn)輸、不易保存等原因造成大量的腐爛,科學(xué)研究表明人畜食用腐爛水果后會造成中毒甚至死亡,使用腐爛瓜果類生物質(zhì)進(jìn)行光合細(xì)菌產(chǎn)氫不僅可以消除資源浪費(fèi)緩解環(huán)境污染,還可以產(chǎn)生清潔的可再生能源,因此成為了可再生能源領(lǐng)域的熱點(diǎn)研究方向。本文研究了不同p H值對HAU-M1光合細(xì)菌生長的影響,做出了其生物量干重對OD660nm值的標(biāo)準(zhǔn)曲線,利用Logistic模型進(jìn)行了光合細(xì)菌生長回歸方程模擬。以蘋果泥、西瓜皮為試驗(yàn)材料進(jìn)行光合細(xì)菌產(chǎn)氫試驗(yàn),研究料液比、p H值調(diào)控、初始p H值、光照度、發(fā)酵溫度、接種量等發(fā)酵工藝對蘋果泥光合生物制氫工藝的影響規(guī)律,使用響應(yīng)面法對蘋果泥光合細(xì)菌產(chǎn)氫試驗(yàn)進(jìn)行優(yōu)化,提出最佳的蘋果泥光合生物制氫工藝參數(shù)。并對瓜果類生物質(zhì)與秸稈類生物質(zhì)光合細(xì)菌產(chǎn)氫進(jìn)行了對比分析。試驗(yàn)結(jié)果表明:1)p H值對光合細(xì)菌生長及產(chǎn)氫過程都有顯著影響,隨著堿性增強(qiáng),光合細(xì)菌生物量逐漸增大,當(dāng)p H值為8時(shí),光合細(xì)菌生物量達(dá)到最大,為543.714 mg/L,最佳接種時(shí)間為84-96h。2)最佳的蘋果泥光合細(xì)菌產(chǎn)氫單因素條件為:蘋果泥與蒸餾水質(zhì)量比為1:4,初始p H值為7,光照度為3000 lx,溫度為30℃,光合細(xì)菌接種量為40%。根據(jù)響應(yīng)面法模型預(yù)測蘋果泥光合細(xì)菌產(chǎn)氫量最大值為714.28m L,此時(shí)試驗(yàn)工藝條件為:初始p H值7.14,光照度3031.33 lx,溫度30.47℃,料液比0.24;最大比產(chǎn)氫量為16.59 m L·g-1,工藝條件為:初始p H值7.14,光照度3032.12 lx,溫度30.50℃,料液比0.21�？蔀楣瞎惿镔|(zhì)光合生物制氫工藝技術(shù)和理論的進(jìn)一步研究提供參考。3)纖維素酶對西瓜皮有很好的酶解作用,經(jīng)纖維素酶處理后,西瓜皮汁液還原糖濃度有顯著提高。p H調(diào)控不利于光合細(xì)菌產(chǎn)氫,p H值調(diào)控可能會減弱光合細(xì)菌活性或者直接將其殺死,對產(chǎn)氫速率和累積產(chǎn)氫量都有很大抑制作用。4)采用響應(yīng)面法BBD模型優(yōu)化玉米秸稈酶解糖化光合細(xì)菌產(chǎn)氫試驗(yàn),獲得的最佳產(chǎn)氫條件為:p H為5.43,溫度為30.8℃,纖維素酶量為70 mg/g。玉米秸稈為5g時(shí)模型最大產(chǎn)氫量為149.39 m L,最大產(chǎn)氫率為29.88m L/g。
[Abstract]:China ranks first in production of melon and fruit in the world, with the representative annual production of apples and watermelons reaching 20 million tons and 70 million tons respectively. A huge amount of fruit production is bound to be transported over long distances because of bad weather. Scientific research shows that human and animal consumption of rotten fruit can lead to poisoning and even death. Using decomposed fruits biomass to produce hydrogen by photosynthetic bacteria can not only eliminate the waste of resources and alleviate environmental pollution. It can also produce clean renewable energy, so it has become a hot research direction in the field of renewable energy. In this paper, the effects of different pH values on the growth of HAU-M1 photosynthetic bacteria were studied, and the standard curve of biomass dry weight to OD660nm value was obtained. The Logistic model was used to simulate the growth of photosynthetic bacteria. The hydrogen production of photosynthetic bacteria was studied by using apple mud and watermelon peel as experimental materials. The control of pH value, initial pH value, light intensity and fermentation temperature were studied. The effects of inoculation and other fermentation techniques on the hydrogen production of apple puree photosynthetic organisms were studied. The response surface method was used to optimize the hydrogen production test of apple puree photosynthetic bacteria. The optimum technological parameters of photosynthetic biological hydrogen production from apple mud were put forward. The hydrogen production of photosynthetic bacteria from melon and fruit biomass was compared with that of straw biomass. The results showed that the value of 1: 1p H had a significant effect on the growth and hydrogen production process of photosynthetic bacteria. With the increase of alkalinity, the biomass of photosynthetic bacteria gradually increased. When pH value was 8, the biomass of photosynthetic bacteria reached the maximum. The optimum conditions for hydrogen production of photosynthetic bacteria from apple mud were as follows: the mass ratio of apple puree to distilled water was 1: 4, initial pH value was 7, light intensity was 3000 LX, temperature was 30 鈩，

本文編號：1692027