【摘要】：為采用生物法治理海洋石油污染,以原油為唯一碳源,從深圳海域5個采樣點取樣,通過富集、涂布平板分離高效石油降解菌,并以復(fù)配、正交等方式構(gòu)建石油降解復(fù)合菌系;通過生理生化實驗和16S r RNA基因序列分析對菌株進行鑒定;采用單因素實驗對復(fù)合菌系降解石油的條件進行優(yōu)化,并使用氣相色譜-質(zhì)譜法(GC-MS)研究其對石油的降解特性。結(jié)果顯示,共分離得到22株高效石油降解菌,對石油的降解率為34.5%-52.2%;由S1-30、S1-38和S2-13構(gòu)建了復(fù)合菌系SQ1,對石油降解率可達68.3%,3株菌分別鑒定為棒桿菌(Corynebacterium sp.)、迪茨氏菌(Dietzia sp.)和拉布倫茨氏菌(Labrenzia sp.);經(jīng)優(yōu)化,SQ1在最適條件下(30℃、p H7.6、石油濃度20 g/L),11 d內(nèi)對石油的去除率高達73.5%;GC-MS結(jié)果表明,復(fù)合菌系SQ1對石油總烷烴的去除率為91.7%,對較難降解的C21-C35烷烴組分的降解率接近100%。研究表明,復(fù)合菌系SQ1在海洋石油污染的生物修復(fù)中具有較強的應(yīng)用潛力。
[Abstract]:In order to treat marine oil pollution by biological method, crude oil was used as the sole carbon source and sampled from five sampling sites in Shenzhen sea area. High efficiency petroleum degrading bacteria were separated by enrichment and coated plate, and the petroleum degradation complex bacteria were constructed by means of compound and orthogonal methods. The strain was identified by physiological and biochemical experiments and 16s r RNA gene sequence analysis. Single factor experiments were used to optimize the degradation conditions of the complex bacteria, and gas chromatography-mass spectrometry (GC-MS) was used to study the degradation characteristics of petroleum. The results showed that 22 strains of high efficiency oil degrading bacteria were isolated and the oil degradation rate was 34.5% / 52.2%. The oil degradation rate of SQ1, was up to 68.3%, and the three strains were identified as Corynebacterium (Corynebacterium sp.), (Dietzia sp.), which was constructed from S1 ~ (30), S _ (1) ~ (38) and S _ (2) ~ (13). Under the optimum conditions (30 鈩，

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