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大亞灣沉積物中可溶性有機氮與蛋白酶和脲酶活性特征及其影響因素

發(fā)布時間:2019-02-12 23:23
【摘要】:為認知海灣沉積物有機氮轉(zhuǎn)化機制及其對富營養(yǎng)化的影響,本文探討了大亞灣表層沉積物可溶性有機氮(DON)、游離氨基酸(DFAA)含量及其關(guān)鍵酶活性的特征。結(jié)果表明,沉積物DON平均含量為50.56×10-6,分別占沉積物可溶性總氮(TDN)和總氮(TN)的64.52%和3.58%,DON是TDN的重要組成部分;DFAA平均含量為8.48×10-6,占DON的20.34%,表明大亞灣沉積物DON有較多易分解組分。DON和DFAA含量均與沉積物TN、總磷(TP)、有機質(zhì)的含量密切相關(guān),呈現(xiàn)出灣沿岸和海水養(yǎng)殖區(qū)較高,而灣中區(qū)域較低的分布趨勢。蛋白酶和脲酶平均活性分別為61.64 mg/kg/d和86.12 mg/kg/d,蛋白酶主要與DFAA密切相關(guān),而脲酶與DON、DFAA均密切相關(guān),脲酶活性大小可以反映沉積物有機氮引起二次污染的風險程度。
[Abstract]:In order to understand the mechanism of organic nitrogen transformation and its effect on eutrophication, the contents of soluble organic nitrogen (DON),) free amino acid (DFAA) and its key enzyme activities in surface sediments of Daya Bay were investigated. The results showed that the average content of DON in sediment was 50.56 脳 10 ~ (-6), accounting for 64.52% and 3.58% of total nitrogen (TDN) and total nitrogen (TN), respectively, which were important components of TDN. The average content of DFAA was 8.48 脳 10 ~ (-6), accounting for 20.34% of DON, indicating that DON in sediment of Daya Bay had more easily decomposed components. The contents of DON and DFAA were closely related to the content of (TP), organic matter in TN,. The distribution of the coastal and mariculture zones was higher, while that of the bay was lower. The average activities of protease and urease were 61.64 mg/kg/d and 86.12 mg/kg/d, respectively, which were closely related to DFAA, while urease was closely related to DON,DFAA. Urease activity can reflect the risk of secondary pollution caused by organic nitrogen in sediments.
【作者單位】: 中國科學院南海海洋研究所熱帶海洋生物資源與生物重點實驗室;中國科學院大學;
【基金】:國家重點基礎(chǔ)研究計劃(973計劃)項目(2015CB452901;2015CB452902;2015CB452905)
【分類號】:P736.41

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