本文選題：TBBPA + 海洋微藻�。� 參考：《中國(guó)海洋大學(xué)》2014年碩士論文

【摘要】：四溴雙酚A(TBBPA)是目前世界上產(chǎn)量和用量最大的溴系阻燃劑，相關(guān)研究表明其對(duì)水生生物具有較強(qiáng)的急性毒性。海洋微藻作為海洋態(tài)系統(tǒng)中的初級(jí)生產(chǎn)者，在維持水生態(tài)的穩(wěn)定與平衡中起著重要作用�；谀壳坝嘘P(guān)TBBPA對(duì)海洋微藻的毒理學(xué)研究還相當(dāng)少見(jiàn)，本文開(kāi)展了TBBPA對(duì)海洋微藻毒性效應(yīng)的相關(guān)研究，為全面評(píng)價(jià)TBBPA對(duì)海洋環(huán)境的影響作出初步探索。主要內(nèi)容分為以下三個(gè)部分，（1）TBBPA對(duì)七種海洋微藻生長(zhǎng)的影響。（2）TBBPA與B[a]P對(duì)塔胞藻和湛江等鞭金藻聯(lián)合毒性效應(yīng)的研究。（3）TBBPA與Cu2+對(duì)塔胞藻和湛江等鞭金藻毒性效應(yīng)的研究。主要結(jié)果如下： 1．TBBPA對(duì)七種海洋微藻生長(zhǎng)的影響 以7種海洋微藻（微擬球藻、塔胞藻、牟氏角毛藻、湛江等鞭金藻、等鞭金藻塔溪）為研究對(duì)象，通過(guò)分析TBBPA處理下海洋微藻相對(duì)生長(zhǎng)率、抑制率的變化，同時(shí)用概率單位—濃度對(duì)數(shù)法求得其96h EC50，來(lái)研究TBBPA對(duì)7種海洋微藻生長(zhǎng)的影響。結(jié)果顯示：TBBPA對(duì)微擬球藻、塔胞藻、牟氏角毛藻、湛江等鞭金藻、等鞭金藻塔溪堤品系的96h EC50分別為2.64mg/L、6.71mg/L、2.59mg/L、4.35mg/L、4.23mg/L，三角褐指藻在濃度為9.83mg/L的TBBPA的作用下在96h時(shí)抑制率為22.8%，亞心形扁藻在濃度14.6mg/L的TBBPA作用下在96h的抑制率僅為17.1%。牟氏角毛藻、微擬球藻為對(duì)TBBPA最為敏感的海洋微藻。 2. TBBPA與B[a]P對(duì)塔胞藻和湛江等鞭金藻聯(lián)合毒性效應(yīng)的研究 本文依據(jù)預(yù)實(shí)驗(yàn)設(shè)計(jì)了合適的污染物配比濃度，先研究TBBPA、B[a]P及TBBPA+B[a]P對(duì)海洋微藻生長(zhǎng)的影響，分別求得三種情況下微藻的96h EC50，然后采用Marking指數(shù)相加法來(lái)評(píng)價(jià)TBBPA與B[a]P對(duì)塔胞藻和湛江等鞭金藻聯(lián)合毒性效應(yīng)。結(jié)果表明：（1）TBBPA對(duì)湛江等鞭金藻的毒性作用屬高毒，B[a]P對(duì)湛江等鞭金藻的作用屬于極高毒。TBBPA與B[a]P在500:3的混合比例下，TBBPA與B[a]P的聯(lián)合毒性指數(shù)AI值為-1.23，小于0，為拮抗作用。（2）TBBPA對(duì)塔胞藻的毒性作用屬高毒，B[a]P對(duì)塔胞藻的作用屬于高毒。在4:1的混合比例下，聯(lián)合毒性指數(shù)AI值為-2.03，小于0，故為拮抗作用。 3. TBBPA與Cu2+對(duì)塔胞藻和湛江等鞭金藻聯(lián)合毒性效應(yīng)的研究 本文依據(jù)預(yù)實(shí)驗(yàn)設(shè)計(jì)了合適的污染物配比濃度，，先研究TBBPA、Cu2+及TBBPA+Cu2+對(duì)海洋微藻生長(zhǎng)的影響，分別求得三種情況下微藻的96h EC50，然后采用Marking指數(shù)相加法來(lái)評(píng)價(jià)TBBPA與Cu2+對(duì)塔胞藻和湛江等鞭金藻聯(lián)合毒性效應(yīng)。結(jié)果表明：（1）Cu2+對(duì)湛江等鞭金藻的作用屬于高毒。在5：1的混合比例下，TBBPA與Cu2+聯(lián)合毒性指數(shù)AI值為-1.23，小于0，為拮抗作用。（2）Cu2+對(duì)塔胞藻的作用屬于中毒。在5:2的混合比例下，TBBPA與Cu2+聯(lián)合毒性指數(shù)AI值為-1.38，小于0，為拮抗作用。
[Abstract]:Tetrabromobisphenol A (TBBPA) is the largest bromine flame retardant in the world at present. Related studies have shown that TBBPA has a strong acute toxicity to aquatic organisms. Marine microalgae, as primary producers in marine systems, play an important role in maintaining the stability and balance of water ecology. Based on the relatively rare toxicological studies of TBBPA on marine microalgae, this paper has carried out related studies on the toxic effects of TBBPA on marine microalgae, in order to evaluate the effects of TBBPA on marine environment. The main contents are as follows: (1) the effect of TBBPA on the growth of seven marine microalgae; (2) the study on the toxic effects of TBBPA and B [a] P on TBBPA and Zhanjiang isopodium. (3) the toxic effects of TBBPA and Cu2 on TBBPA and Zhanjiang isopodium. The main results are as follows: 1. The effects of TBBPA on the growth of seven marine microalgae were studied. The effects of TBBPA on the growth of seven marine microalgae were studied by analyzing the changes of the relative growth rate and inhibition rate of marine microalgae under TBBPA treatment, and the 96 h EC50 was obtained by using probabilistic unit concentration logarithm method to study the effect of TBBPA on the growth of seven marine microalgae. The results showed that the effects of TBBPA on Chlorella microphylla, Phaeocystis monocystis, Cham's Corydophyta, Zhanjiang isopodium were studied. The 96 h EC50 of TBBPA was 2.64 mg / L 6.71 mg / L 2.59 mg / L 4.35 mg / L 4.23 mg / L, the inhibition rate of Phaeopsis triangularis was 22.8 at 96 h under the action of 9.83 mg / L TBBPA, and the inhibition rate of TBBPA at 14.6 mg / L was 17.1mg / L at 96 h. Chlorella mongolica is the most sensitive marine microalgae to TBBPA. Effects of TBBPA and B [a] P on the combined toxicity of TBBPA and B [a] P on the growth of marine microalgae, the suitable concentration of pollutants was designed based on the pre-experiment. The effects of TBBPA B [a] P and TBBPA B [a] P on the growth of marine microalgae were studied firstly, and the effects of TBBPA B [a] P and TBBPA B [a] P on the growth of marine microalgae were studied. The 96 h EC50 of microalgae was obtained under three conditions, and the combined toxicity of TBBPA and B [a] P to Phaeophyta and Zhanjiang isopodium was evaluated by Marking index addition method. The results showed that: (1) the toxic effect of TBBPA on Isochrysis zhanjiang belongs to the highly toxic B [a] P, which belongs to the extremely high toxicity. TBBPA and B [a] P in the mixture ratio of 500: 3 and B [a] P, the joint toxicity index AI of TBBPA and B [a] P is -1.23, less than 0, which is antagonistic effect. (2) the toxic effect of TBBPA on TBBPA belongs to high toxic B [a] P. Under the mixture ratio of 4:1, the AI value of joint toxicity index was -2.03, less than 0, so it was antagonistic. Effects of TBBPA and Cu _ 2 on the combined toxicity of TBBPA and Cu _ 2 on the growth of marine microalgae the suitable concentration of pollutants was designed based on the pre-experiment. The effects of TBBPA Cu2 and TBBPA Cu2 on the growth of marine microalgae were studied. The 96 h EC50 of microalgae was obtained under three conditions, and the combined toxicity of TBBPA and Cu2 to Phaeophyta and Zhanjiang isopodium was evaluated by Marking index addition method. The results showed that: (1) the effect of Cu _ 2 on isopodium zhanjiang belongs to high toxicity. The combined toxicity index (AI) of TBBPA and Cu2 was -1.23 at 5:1, which was antagonistic. (2) the effect of Cu2 on TBBPA and Cu2 was toxic. The combined toxicity index (AI) of TBBPA and Cu2 was -1.38, less than 0 at 5:2, which was antagonistic.
【學(xué)位授予單位】：中國(guó)海洋大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：X171.5;X55

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