【摘要】：白云石成因問題是地質(zhì)學(xué)上長期懸而未決的難題之一.近年來,微生物誘導(dǎo)白云石沉淀逐漸成為白云石成因的重要理論之一,但其中微生物的作用機理遠(yuǎn)未探明.現(xiàn)生白云石主要分布于高鹽環(huán)境,該環(huán)境中的優(yōu)勢菌群為嗜鹽菌,包括嗜鹽細(xì)菌和嗜鹽古菌.因而此次選取三株嗜鹽古菌Natrinema sp.J7-1、Natrinema sp.J7-3和Natrinema sp.LJ7,研究其誘導(dǎo)白云石沉淀的能力,并對比不同細(xì)胞濃度對白云石沉淀的影響,以期更深入地了解微生物在白云石形成中的作用.通過X射線衍射(XRD)檢測沉淀物的物相,利用掃描電子顯微鏡(SEM)觀察所得礦物形態(tài),同時輔以能量色散譜分析(EDS)分析礦物的元素組成.實驗結(jié)果表明三株嗜鹽古菌皆可誘導(dǎo)球型、啞鈴型、花椰菜型以及球形聚集體等白云石的形成,且在較高細(xì)胞濃度下誘導(dǎo)形成的礦物中白云石含量增多.分析表明細(xì)胞濃度的增加會導(dǎo)致細(xì)胞表面羧基含量的增加,從而為白云石的沉淀提供更多的成核位點,有利于Mg進(jìn)入礦物晶格,從而誘導(dǎo)白云石沉淀,本結(jié)果進(jìn)一步提高了對微生物白云石成因機理的認(rèn)識.
[Abstract]:The genesis of dolomite is one of the outstanding geological problems for a long time. In recent years, microbial induced dolomite precipitation has gradually become one of the important theories of dolomite genesis, but the mechanism of microbial action is far from clear. The present dolomite is mainly distributed in high salt environment, and the dominant flora in this environment is halophilic bacteria, including halophilic bacteria and halophilic archaea. Therefore, three strains of halophilic archaea Natrinema sp.J7-1,Natrinema sp.J7-3 and Natrinema sp.LJ7, were selected to study their ability to induce dolomite precipitation, and the effects of different cell concentrations on dolomite precipitation were compared in order to understand the role of microorganisms in dolomite formation. The phase of precipitates was detected by X-ray diffraction (XRD), and the mineral morphology was observed by scanning electron microscope (SEM). At the same time, the elemental composition of minerals was analyzed by energy dispersion spectroscopy (EDS). The results showed that all three halophilic archaea could induce the formation of dolomite such as ball type, dumbbell type, cauliflower type and spherical aggregate, and the content of dolomite in the minerals induced by high cell concentration increased. The analysis shows that the increase of cell concentration will lead to the increase of carboxy content on the cell surface, thus providing more nucleation sites for dolomite precipitation, which is beneficial for Mg to enter the mineral lattice and induce dolomite precipitation. This result further improves the understanding of the genetic mechanism of microbial dolomite.
【作者單位】： 中國地質(zhì)大學(xué)生物地質(zhì)與環(huán)境地質(zhì)國家重點實驗室;中國地質(zhì)大學(xué)環(huán)境學(xué)院;
【基金】：國家自然科學(xué)基金項目(Nos.41502317,41572323,41130207)
【分類號】：P571

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