微流控作為一種新興技術,被廣泛應用于化學和生物分析、醫(yī)學研究、疾病早期診斷等領域。它能夠處理微升/納升乃至更小體積的液體生物樣品和試劑,實現(xiàn)樣品輸運、泵送、混合、細胞處理,生物反應以及生物檢測等,并且新的功能仍然在不斷涌現(xiàn)。芯片實驗室作為微流控技術的延伸,在一個小芯片上集成了生物傳感和樣品前處理等更多功能。對比傳統(tǒng)的分析方法,微流控和芯片實驗室具有更小的尺寸,因此允許使用少量試劑及分析少量樣品,并實現(xiàn)了單一芯片上的多功能集成,從而避免潛在的交叉污染和人為的操作錯誤。微流控技術的其他優(yōu)勢還包括低成本,一次性使用,快速分析等,其可在幾分鐘內(nèi)獲得結(jié)果,而不是同傳統(tǒng)分析方法的幾小時或者幾天。由于其獨特的功能和巨大的應用潛力,各大公司,學校及政府都大量投入,進行深入廣泛的研究探索。由于SAW可用于開發(fā)運輸,泵送,混合,霧化,液滴生成,生物傳感,顆粒(細胞)集聚,分揀和分離等,近些年,表面聲波(Surface Acoustic Wave,SAW)被用于開發(fā)基于SAW單一原理的微流體及片上實驗室,其結(jié)構(gòu)和工藝簡化,成本低,將在老齡化社會的健康呵護和醫(yī)療應用方面發(fā)揮重要作用。本人博士期間研究的目標是探...

【文章頁數(shù)】：138 頁

【學位級別】：博士

【文章目錄】：
Abstract
摘要
List of Abbreviations
Acknowledgements
Chapter 1 Introduction
    1.1 Literature review
    1.2 Microfluidics
    1.3 Microfluidics theory
    1.4 Microfluidic channel designing and materials
    1.5 Cryopreservation technology
    1.6 Common methods for cryopreservation
    1.7 Acoustic waves
    1.8 Piezoelectric materials and piezoelectricity
Chapter 2 Surface Acoustic Wave
    2.1 Introduction
    2.2 Acoustic radiation force and acoustic streaming
        2.2.1 Derivation of acoustic radiation pressure
        2.2.2 Acoustic radiation force
    2.3 SAW acoustic streaming
        2.3.1 Numerical solution of acoustic streaming
Chapter 3 SAW Device Simulation, Designing and Experimental Testing
    3.1 Introduction
    3.2 Design of SAW device
    3.3 Simulation of SAW device
    3.4 Geometry settings
    3.5 Subdomain settings
        3.5.1 Boundary settings
    3.6 Fabrication of SAW Device
        3.6.1 Interdigital transducer (IDT) designing
        3.6.2 IDT metal electrode preparation
        3.6.3 PDMS bonding with SAW substrate
    3.7 SAW characterization setup
Chapter 4 SAW based Tensiometry
    4.1 Introduction
    4.2 Operating principle
    4.3 Fabrication and characterization
    4.4 Simulation and experimental results
    4.5 Results and discussions
    4.6 Conclusions
Chapter 5 SAW based Cell Pumping and Counting
    5.1 Introduction
    5.2 SAW pumped lensless microfluidic imaging system
    5.3 Theoretical analysis of SAW device
    5.4 SAW device design for cell counting
    5.5 Temporal-differencing based cell detection and counting
    5.6 Cell counting
    5.7 Experimental section: System setup
    5.8 Experimental section: Sample preparation
    5.9 SAW Device Characterization
    5.10 Results of cells detection and counting
    5.11 Conclusions
Chapter 6 SAW Lab-on-chip for Stem Cells Cryopreservation
    6.1 Introduction
    6.1 Working principle and operation details
    6.2 Experimental section
        6.2.1 Device design and fabrication
        6.2.2 Cell culture
        6.2.3 Cryopreservation solutions
        6.2.4 CPA loading and unloading by conventional multistep method
        6.2.5 SAW based multistep loading/unloading method
        6.2.6 Ultra-rapid cooling of hUCM-MSCs by liquid nitrogen quenching
        6.2.7 Statistical analysis
    6.3 Results and discussion
        6.3.1 Characteristics of SAW-enabled micromixer
        6.3.2 Post-cryopreservation cell viability and proliferation
    6.4 Acoustic streaming simulation
    6.5 Simulation of CPA and water exchange across cell membrane
    6.6 Conclusion
Chapter 7 Summary and Future Work
    7.1 Summaries
    7.2 Future work
References
List of Publications



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