微流芯片中消逝波激勵(lì)的熒光輻射特性研究
發(fā)布時(shí)間:2019-08-07 15:47
【摘要】:將石英裸光纖植入聚二甲基硅氧烷基片的微流道中,采用沿光纖軸向光抽運(yùn)、消逝場(chǎng)激勵(lì)染料分子的方式,在基片微流道中獲得均勻的熒光輻射.實(shí)驗(yàn)發(fā)現(xiàn),熒光輻射的強(qiáng)度隨光纖軸向距離的增加而衰減,光纖包層溶液折射率越大,熒光沿光纖軸向的衰減越突出;包層溶液中染料濃度越大,熒光沿光纖軸向的衰減也越突出;通過選擇適當(dāng)?shù)陌鼘尤芤赫凵渎室约叭玖蠞舛瓤梢垣@得沿光纖軸向接近均勻的熒光輻射.用消逝波激勵(lì)熒光的輻射理論計(jì)算了熒光光強(qiáng)沿光纖軸向的變化,計(jì)算結(jié)果與實(shí)驗(yàn)符合較好.在此基礎(chǔ)上,設(shè)計(jì)并制作了一種具有三個(gè)通道的聚二甲基硅氧烷基片,通過在三個(gè)微流道中分別注入染料濃度均為0.1 mmol的羅丹明640、羅丹明B及羅丹明6 G的乙醇染料溶液,采用沿光纖軸向消逝波光激勵(lì)方式,在一塊聚二甲基硅氧烷基片上同時(shí)實(shí)現(xiàn)了三個(gè)不同波段的熒光輻射.
[Abstract]:Quartz bare fiber was implanted into the microchannel of polydimethyl siloxane substrate. Uniform fluorescence radiation was obtained in the substrate microchannel by means of axial optical pumping along the optical fiber and excitation of dye molecules by vanishing field. It is found that the intensity of fluorescence radiation decays with the increase of axial distance of optical fiber, the larger the refractive index of optical fiber coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is, the higher the dye concentration in the coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is. By selecting the appropriate refractive index of coating solution and dye concentration, the fluorescence radiation close to uniform along the axial direction of optical fiber can be obtained. The variation of fluorescence intensity along the axial direction of optical fiber is calculated by using the radiation theory of vanishing wave excited fluorescence, and the calculated results are in good agreement with the experimental results. On this basis, a kind of polydimethyl siloxane substrate with three channels was designed and fabricated. Three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions with dye concentration of 0.1 mmol into three microchannels, and using the optical excitation mode of vanishing wave along the axial direction of the optical fiber, three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate at the same time. The fluorescence radiation in three different bands was realized at the same time by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions into three microchannels.
【作者單位】: 云南大學(xué)物理系;
【基金】:國(guó)家自然科學(xué)基金(批準(zhǔn)號(hào):11404282,61465014) 中國(guó)科學(xué)院西部之光人才培養(yǎng)項(xiàng)目 云南大學(xué)中青年骨干教師培養(yǎng)項(xiàng)目資助的課題~~
【分類號(hào)】:TN492
本文編號(hào):2524041
[Abstract]:Quartz bare fiber was implanted into the microchannel of polydimethyl siloxane substrate. Uniform fluorescence radiation was obtained in the substrate microchannel by means of axial optical pumping along the optical fiber and excitation of dye molecules by vanishing field. It is found that the intensity of fluorescence radiation decays with the increase of axial distance of optical fiber, the larger the refractive index of optical fiber coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is, the higher the dye concentration in the coating solution is, the more prominent the attenuation of fluorescence along the axial direction of optical fiber is. By selecting the appropriate refractive index of coating solution and dye concentration, the fluorescence radiation close to uniform along the axial direction of optical fiber can be obtained. The variation of fluorescence intensity along the axial direction of optical fiber is calculated by using the radiation theory of vanishing wave excited fluorescence, and the calculated results are in good agreement with the experimental results. On this basis, a kind of polydimethyl siloxane substrate with three channels was designed and fabricated. Three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions with dye concentration of 0.1 mmol into three microchannels, and using the optical excitation mode of vanishing wave along the axial direction of the optical fiber, three different bands of fluorescence radiation were realized on a polydimethyl siloxane substrate at the same time. The fluorescence radiation in three different bands was realized at the same time by injecting Rhodamine 640, rhodamine B and rhodamine 6 G ethanol dye solutions into three microchannels.
【作者單位】: 云南大學(xué)物理系;
【基金】:國(guó)家自然科學(xué)基金(批準(zhǔn)號(hào):11404282,61465014) 中國(guó)科學(xué)院西部之光人才培養(yǎng)項(xiàng)目 云南大學(xué)中青年骨干教師培養(yǎng)項(xiàng)目資助的課題~~
【分類號(hào)】:TN492
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