【摘要】：多孔硅(PSi)是通過單晶硅上腐蝕出來具有海綿狀結(jié)構(gòu)的一維納米光子晶體材料,PSi經(jīng)過溶液處理后,其熒光背景低并且紅外透光,易于制備成各種光子器件。利用不同機(jī)理的熒光與PSi光子器件結(jié)合,生物檢測靈敏度被大大提升,可以使制備出的PSi光學(xué)生物傳感器及生物芯片的性能更加優(yōu)異。本論文主要將PSi優(yōu)異的光學(xué)特性與半導(dǎo)體膠體量子點(QDs)的良好的生物特性相結(jié)合,設(shè)計并制備出高靈敏度的熒光光學(xué)生物傳感器,并進(jìn)行有效的生物檢測,探索并制備出基于半導(dǎo)體膠體量子點熒光的多孔硅新型光學(xué)生物傳感器。以下是本論文的主要研究內(nèi)容和結(jié)論:1.通過偶聯(lián)劑1-乙基-(3-二甲基氨基丙基)碳酰二亞胺鹽酸鹽(EDC)和N-羥基硫代琥珀酰亞胺(sulfo-NHS)激活水溶性CdSe/ZnS QDs的羧基,根據(jù)半導(dǎo)體膠體QDs的熒光峰設(shè)計出相應(yīng)的PSi光子器件,半導(dǎo)體膠體QDs成功的與功能化后帶氨基的PSi共價連接,增強(qiáng)了PSi內(nèi)QDs的熒光強(qiáng)度。2.為了驗證基于半導(dǎo)體膠體QDs熒光的多孔硅光學(xué)生物傳感器的可行性,我們將半導(dǎo)體膠體QD標(biāo)記的生物素、磷酸鹽緩沖液(PBS)、及無標(biāo)記的生物素添加到鏈霉親和素修飾的PSi上,通過熒光對比,實驗結(jié)果表明只有半導(dǎo)體膠體QD標(biāo)記的生物素添加到鏈霉親和素修飾的PSi內(nèi),才會產(chǎn)生熒光現(xiàn)象。從而證明基于量子點熒光的多孔硅光學(xué)生物傳感器的可行性,進(jìn)而對不同濃度的SA進(jìn)行檢測,其檢出限為100pM。3.為了擴(kuò)大利用半導(dǎo)體膠體量子點熒光的多孔硅光學(xué)生物傳感器的應(yīng)用,我們將該生物傳感器檢測自制的包蟲病抗原。首先將半導(dǎo)體膠體QD標(biāo)記包蟲病抗體,然后與不同濃度的包蟲病抗原修飾PSi進(jìn)行生物反應(yīng)。結(jié)果表明包蟲病抗原濃度與PSi內(nèi)QD免疫反應(yīng)結(jié)合的熒光強(qiáng)度成線性關(guān)系,檢出限為500fg/ml。
[Abstract]:Porous silicon (PSi) is a one-dimensional nano-photonic crystal material with spongy structure which is etched on monocrystalline silicon. After solution treatment, PSi has a low fluorescence background and infrared transmittance, so it is easy to be fabricated into various photonic devices. With the combination of different mechanisms of fluorescence and PSi photon devices, the sensitivity of biological detection is greatly improved, and the performance of the PSi optical biosensor and biochip can be made more excellent. In this thesis, the excellent optical properties of PSi and the good biological properties of semiconductor colloid quantum dot (QDs) are combined to design and prepare a high sensitivity fluorescent optical biosensor, and to carry out effective biological detection. A novel porous silicon biosensor based on semiconductor colloidal quantum dot fluorescence was developed. The following are the main contents and conclusions of this paper: 1. The carboxyl group of water-soluble CdSe/ZnS QDs was activated by coupling agent 1-ethyl-(3-dimethylaminopropyl) carbonyl diimine hydrochloride (EDC) and N-hydroxythiosuccinimide (sulfo-NHS). The corresponding PSi photon devices were designed according to the fluorescence peak of semiconductor colloid QDs. The semiconductor colloid QDs was successfully covalently connected with the functionalized PSi with amino group, which enhanced the fluorescence intensity of QDs in PSi. 2. In order to verify the feasibility of porous silicon optical biosensor based on semiconductor colloid QDs fluorescence, we labeled biotin and phosphate buffer (PBS), with semiconductor colloid QD. And non-labeled biotin was added to streptavidin-modified PSi. The results showed that only when the biotin labeled with semiconductor colloid QD was added to streptavidin-modified PSi, the fluorescence phenomenon could be produced. Thus, the feasibility of porous silicon optical biosensor based on quantum dot fluorescence is proved, and the detection limit of SA in different concentrations is 100pM.3. In order to expand the application of porous silicon optical biosensor using semiconductor colloid quantum dot fluorescence, we used the biosensor to detect the self-made antigen of hydatid disease. Firstly, semiconductor colloid QD was used to label the antibody against hydatid disease and then reacted with different concentrations of hydatid antigen modified PSi. The results showed that there was a linear relationship between the concentration of echinococcosis antigen and the fluorescence intensity of QD immunoreactivity in PSi, and the detection limit was 500 fg / ml.
【學(xué)位授予單位】：新疆大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O471.1;TP212.3

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本文編號：2454317