本文選題：細(xì)胞檢測 + 微流控芯片��； 參考：《西安理工大學(xué)》2017年碩士論文

【摘要】：利用微流控芯片和無透鏡CMOS圖像傳感器進(jìn)行流式細(xì)胞的圖像采集,是近年來的一個研究熱點(diǎn),但是,由于未經(jīng)透鏡放大,采集到的細(xì)胞圖像分辨率較低、細(xì)節(jié)信息少;同時,微流控芯片的控制效果不理想,導(dǎo)致細(xì)胞黏連、重影現(xiàn)象嚴(yán)重,因此,研究流式細(xì)胞檢測系統(tǒng)中,細(xì)胞圖像的采集對細(xì)胞的分析檢測具有重要意義。本文運(yùn)用微流控技術(shù)和CMOS圖像傳感技術(shù)進(jìn)行流式細(xì)胞的成像研究,主要內(nèi)容有:三維聚焦微流控芯片的設(shè)計(jì)、芯片表面粗糙度的分析和改善、圖像傳感器和PDMS封接成微流控芯片的工藝研究、細(xì)胞圖像采集過程的硬件實(shí)現(xiàn)以及原始圖像預(yù)處理。首先對微流控芯片的聚焦原理和設(shè)計(jì)過程進(jìn)行研究,分析總結(jié)具有二維聚焦功能的三叉型微流控芯片的結(jié)構(gòu)特點(diǎn)和缺陷,在此基礎(chǔ)上,設(shè)計(jì)制作出中間帶擋板的三叉型微流控芯片,能夠完成垂直向下方向上的細(xì)胞聚焦;其次,摸索完善微流控芯片的制作工藝,對影響芯片內(nèi)壁表面粗糙度的關(guān)鍵因素進(jìn)行分析,并提出解決方法;接下來,研究用CMOS圖像傳感器的玻璃蓋板代替有機(jī)玻璃,和PDMS鍵合封接的工藝流程,通過理論分析和實(shí)驗(yàn)對比,制作出基于OV5640圖像傳感器的微流控芯片;最后,完成了細(xì)胞圖像的采集、存儲、顯示等過程的硬件實(shí)現(xiàn),并利用FPGA的片內(nèi)ROM存儲器進(jìn)行圖像存儲,對采集到的原始圖像進(jìn)行預(yù)處理,降低數(shù)據(jù)處理量。通過本文的研究,改善了微流控芯片的聚焦性能和控制效果,并搭建出基于FPGA硬件平臺的流式細(xì)胞圖像采集系統(tǒng),為進(jìn)一步降低系統(tǒng)體積,提高處理速度做出貢獻(xiàn)。
[Abstract]:Using microfluidic chip and lensless CMOS image sensor to collect flow cell image is a hot research topic in recent years. However, because of the unamplified lens, the cell image has low resolution and less detail information. The control effect of microfluidic chip is not ideal, which leads to cell adhesion and heavy double image phenomenon. Therefore, it is very important to study cell image collection in flow cytometry system. In this paper, flow cytometry (FCM) imaging is studied by using microfluidic technology and CMOS image sensing technology. The main contents are as follows: design of 3D focused microfluidic chip, analysis and improvement of surface roughness of microfluidic chip. The process of sealing microfluidic chip with image sensor and PDMS, the hardware realization of cell image acquisition and the preprocessing of original image are studied. Firstly, the focusing principle and design process of microfluidic chip are studied, and the structural characteristics and defects of tri-forked microfluidic chip with two-dimensional focusing function are analyzed and summarized. A tri-forked microfluidic chip with a middle baffle is designed and fabricated, which can focus the cells vertically down. Secondly, the fabrication process of the microfluidic chip is explored and the key factors affecting the surface roughness of the inner wall of the chip are analyzed. Then, the process of using glass cover plate of CMOS image sensor instead of plexiglass and bonding and sealing with PDMS is studied. The microfluidic chip based on OV5640 image sensor is fabricated by theoretical analysis and experimental comparison. Finally, the hardware realization of cell image acquisition, storage, display and so on is completed. The image is stored by ROM memory in FPGA, and the original image is preprocessed to reduce the data processing capacity. Through the research in this paper, the focusing performance and control effect of the microfluidic chip are improved, and a flow cell image acquisition system based on FPGA hardware platform is built, which contributes to further reducing the volume of the system and increasing the processing speed.
【學(xué)位授予單位】：西安理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：TH789;TN492

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本文編號：1970340