發(fā)布時間：2018-04-29 14:38

  本文選題：床旁快速檢測 + 心肌肌鈣蛋白I��； 參考：《南京郵電大學(xué)》2015年碩士論文

【摘要】：床旁快速診斷具有快速、操作簡單和節(jié)約綜合成本等優(yōu)點(diǎn),因而廣泛應(yīng)用在診所和患者家中。為了增加檢測設(shè)備的便攜性,Whitesides等人提出了基于紙為基底的紙質(zhì)微流控芯片,具有低成本、低檢測背景、較好的生物相容性、易于折疊和后處理方法簡單等優(yōu)點(diǎn)。心肌肌鈣蛋白I作為理想的心臟標(biāo)志物,目前臨床檢測主要為中心實(shí)驗室檢測:需要大型檢測儀器和專業(yè)人員,不適合臨床快速診斷。而基于紙質(zhì)微流控器件的床旁快速診斷滿足檢測要求�；谝陨媳尘�,本論文提出一種基于噴墨印刷技術(shù)制作紙質(zhì)微流控器件的方法,且利用制作的紙質(zhì)微流控器件用于c Tn I的床旁快速檢測。在圖案化的紙質(zhì)微流控芯片上,利用噴墨打印技術(shù)沉積捕獲抗體,制作全打印生物檢測器件�；驹硎菬晒釶S納米微球作為標(biāo)記物,利用熒光顯微鏡獲得檢測區(qū)域熒光信號,計算得到c Tn I的定量結(jié)果。本論文通過研究,得到最佳抗體配對為m Ab1-16A11與m Ab2-19C7,甲苯與PDMS按質(zhì)量比10:1進(jìn)行混合得到的溶液滿足噴墨打印機(jī)的要求,最終結(jié)果表明這種紙質(zhì)微流控檢測器件可以用于進(jìn)行生物檢測,且能達(dá)到1 ng/m L的檢測限。本論文還研究銀電極的制作與優(yōu)化:通過研究在NC膜上噴墨打印銀納米粒子制備銀電極的幾個重要影響因素:基板溫度、點(diǎn)間距、打印層數(shù)和后處理時間得出,當(dāng)基板溫度為40℃,點(diǎn)間距為20μm,后處理時間為90分鐘時,得到的銀電極具有較小的電阻值,可以與紙質(zhì)微流控器件結(jié)合進(jìn)行電化學(xué)檢測。
[Abstract]:Rapid bedside diagnosis has the advantages of fast operation, simple operation and cost saving, so it is widely used in clinics and patients' homes. In order to increase the portability of the detection equipment, Whitesides et al proposed a paper microfluidic chip based on paper substrate, which has the advantages of low cost, low detection background, good biocompatibility, easy folding and simple post-processing. Cardiac troponin I is an ideal cardiac marker. At present, the clinical detection is mainly a central laboratory test. It needs large detection instruments and professionals, so it is not suitable for rapid clinical diagnosis. The bed-side rapid diagnosis based on paper microfluidic device can meet the requirement of detection. Based on the above background, this paper presents a method to fabricate paper microfluidic devices based on inkjet printing technology, and makes use of the paper microfluidic devices for fast bedside detection of c TN I. In a patterned paper microfluidic chip, an all-print biological detection device was fabricated by using inkjet printing technology to deposit and capture antibodies. The basic principle is that fluorescent PS nanospheres are used as markers, fluorescence microscope is used to obtain fluorescence signal of detection region, and quantitative results of cTnI are calculated. In this paper, the best antibody pairing is m Ab1-16A11 and mAb2-19C7. The mixture of toluene and PDMS meets the requirements of inkjet printer at 10:1 mass ratio. The results show that the paper microfluidic detection device can be used for biological detection, and the detection limit of 1 ng/m L can be reached. In this paper, we also study the fabrication and optimization of silver electrode: by studying several important factors that affect the preparation of silver electrode by ink jet printing silver nanoparticles on NC film: substrate temperature, point spacing, number of printing layers and post-processing time. When the substrate temperature is 40 鈩，

本文編號：1820357