本文選題：微流控芯片　切入點：乳腺癌細胞　出處：《浙江大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：基于微流控芯片的細胞/組織芯片又稱人體芯片(body-on-a-chip),是把一種細胞或多種細胞培養(yǎng)于微流控芯片通道內(nèi),模擬人體內(nèi)微環(huán)境,實現(xiàn)細胞在體外的長期培養(yǎng),可用于藥物篩選,為解決傳統(tǒng)藥物篩選實驗耗時長、耗費大的問題提供了一種新方法。本課題設(shè)計并制作了基于微流控的乳腺癌細胞三維培養(yǎng)芯片,該芯片可用于初步的藥篩實驗。芯片選用了無毒性,且具有良好可塑性和光透性的PDMS作為制作材料,通過多組實驗對比,確定芯片結(jié)構(gòu)為不帶夾膜的雙層結(jié)構(gòu),細胞培養(yǎng)池選用能減少流體剪切力影響的圓形細胞池。利用注射泵回抽培養(yǎng)基作為芯片的通液模式,不僅減少了氣泡的產(chǎn)生,其回收液還可用于藥效檢測。在芯片的制作方法上,創(chuàng)新性地采用了成本低廉,制作簡單并且可以快速成型的3D打印技術(shù)。芯片上的細胞培養(yǎng)結(jié)果表明該芯片可以很好地用于乳腺癌細胞的二維培養(yǎng)和三維培養(yǎng)。在微流控的作用下,芯片上的三維細胞可培養(yǎng)7天左右,直至形成腔體結(jié)構(gòu),微流控為細胞培養(yǎng)提供了營養(yǎng)輸送的同時幫助排除廢液。此外,當(dāng)流速控制在50μl/h以下時,不同大小的流體剪切力在短期內(nèi)對細胞生長影響不大,細胞生長狀態(tài)類似。藥篩實驗中,將濃度為0.02μmol/ml紫杉醇作用于芯片細胞,24小時后的細胞熒光圖像結(jié)果顯示,紫杉醇對三維乳腺癌細胞生長有抑制作用。
[Abstract]:The cell / tissue chip based on microfluidic chip, also called human body-on-a-chip-chip-chip, is to culture one or more cells in microfluidic chip channels, simulate the microenvironment of human body, and realize long-term cell culture in vitro, which can be used for drug screening. In order to solve the problem of long time and high cost in traditional drug screening experiments, a novel microfluidic based three-dimensional culture chip for breast cancer cells was designed and fabricated. The chip can be used in the preliminary drug screening experiment. The chip uses PDMS, which is non-toxic and has good plasticity and light permeability, as the fabrication material. Through the comparison of many groups of experiments, it is determined that the chip structure is a double-layer structure without clip film. The cell culture pool is a circular cell pool which can reduce the effect of fluid shear stress. Using the injection pump back pumping medium as the liquid mode of the chip not only reduces the production of bubbles. The recycled liquid can also be used for drug efficacy testing. In the fabrication of the chip, the cost of the chip is innovatively adopted. The results of cell culture on the chip show that the chip can be used in two-dimensional and three-dimensional culture of breast cancer cells. The three-dimensional cells on the chip can be cultured for about 7 days until the cavity structure is formed. Microfluidic provides nutrition transport for cell culture and helps to remove waste liquid. In addition, when the flow rate is less than 50 渭 l / h, Fluid shear stress of different sizes had little effect on cell growth in a short period of time, but the cell growth state was similar. In the drug screening experiment, the fluorescence images of cells exposed to paclitaxel at a concentration of 0.02 渭 mol/ml for 24 hours showed that, Paclitaxel inhibits the growth of three dimensional breast cancer cells.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TN492;R737.9

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