本文選題：電泳芯片　切入點(diǎn)：非接觸電導(dǎo)檢測　出處：《華中科技大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：全氟辛烷磺�；衔�(PFOS)和全氟辛酸銨(PFOA)衍生物得到的共聚物在當(dāng)今工業(yè)中得到廣泛應(yīng)用。研究表明,PFOS和PFOA類物質(zhì)是危害人類健康的環(huán)境污染物,是全球斯德哥爾摩公約重點(diǎn)關(guān)注對(duì)象,但是目前還未找到完全合適的替代品,因此研究PFOS和和PFOA等全氟化合物的檢測技術(shù)具有重要意義。目前PFOA及PFOS檢測技術(shù)如光學(xué)檢測、質(zhì)譜檢測等對(duì)測試樣品要求高,且檢測裝置龐大,不利于現(xiàn)場測量。本文針對(duì)現(xiàn)場檢測含氟化合物,提出非接觸電導(dǎo)檢測的微芯片電泳系統(tǒng)。論文首先比較了現(xiàn)有全氟化合物檢測技術(shù),再重點(diǎn)評(píng)述微流控芯片及其非接觸電導(dǎo)檢測技術(shù)的研究進(jìn)展的基礎(chǔ)上,用于分析全氟化合物提出的的便攜式芯片電泳非接觸電導(dǎo)檢測儀器。其次,論文研究了非接觸電導(dǎo)檢測器的電路設(shè)計(jì),包括檢測器電路構(gòu)成、激勵(lì)信號(hào)選取和信號(hào)檢測電路設(shè)計(jì)。本文選擇高速函數(shù)發(fā)生器MAX038為核心并采用微控制器STM32作為電壓控制系統(tǒng),為檢測器提供正弦激勵(lì)交流信號(hào);設(shè)計(jì)了基于選擇電流反饋法的初級(jí)I-V轉(zhuǎn)換電路、信號(hào)放大和整流電路,通過軟件仿真,驗(yàn)證了非接觸電導(dǎo)檢測器的檢測電路設(shè)計(jì)的可行性,并制作了檢測電路PCB板,用于非接觸電導(dǎo)檢測。最后,論文構(gòu)建了微流控芯片電泳非接觸電導(dǎo)檢測儀器試驗(yàn)測試平臺(tái)。通過兩電極非接觸檢測器電極系統(tǒng)的制作和電路測試,完成了不同溶液濃度的非接觸電導(dǎo)測試。研究表明,本文設(shè)計(jì)的非接觸電導(dǎo)檢測器可有效感應(yīng)出濃度變化范圍為10-2mol/L~10-4 mol/L的變化,初步實(shí)現(xiàn)了預(yù)期的芯片電泳非接觸電導(dǎo)檢測裝置的基本要求。
[Abstract]:The copolymers derived from perfluorooctanesulfonyl compounds (PFOS) and perfluorooctanoic acid (PFOAA) derivatives have been widely used in industry today. Studies have shown that perfluorooctanesulfonyl compounds and PFOA compounds are environmental pollutants harmful to human health. Is the focus of the Stockholm Convention, but no suitable alternatives have been found, so it is important to study the detection technology of perfluorinated compounds such as PFOS and PFOA. Currently, PFOA and PFOS detection techniques such as optical detection, Mass spectrometry has a high requirement for testing samples, and the equipment is too large, which is not good for field measurement. In this paper, fluorine compounds are detected in the field. A microchip electrophoresis system for contactless conductance detection is proposed. Firstly, the existing perfluorocarbon detection techniques are compared, and then the research progress of microfluidic chips and their non-contact conductance detection techniques are reviewed. A portable chip electrophoresis non-contact conductance detector proposed by perfluorinated compounds is analyzed. Secondly, the circuit design of non-contact conductance detector is studied, including the circuit structure of the detector. In this paper, the high speed function generator (MAX038) is chosen as the core and the microcontroller STM32 is used as the voltage control system to provide sinusoidal AC signal for the detector. The primary I-V conversion circuit, signal amplifying and rectifying circuit based on selective current feedback method are designed. The feasibility of the design of non-contact conductance detector detection circuit is verified by software simulation, and the PCB board of the detection circuit is made. Finally, a microfluidic chip electrophoretic non-contact conductivity testing instrument test platform was constructed. The fabrication and circuit test of the two-electrode non-contact detector electrode system were carried out. The contactless conductance measurements of different solution concentrations have been completed. The results show that the contactless conductance detector designed in this paper can effectively detect the change of concentration in the range of 10-2mol / L 10-4 mol/L. The basic requirements of the expected non-contact conductivity detector for chip electrophoresis have been preliminarily realized.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TN492;R927

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