本文關(guān)鍵詞：基于多孔碳材料的酶生物燃料電池酶電極的研究　出處：《湖南大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 酶生物燃料電池 多孔碳材料 二茂鐵 中間體 葡萄糖氧化酶

【摘要】：酶生物燃料電池(EBFC)是一類以生物質(zhì)燃料為原料,生物酶為催化劑將化學(xué)能轉(zhuǎn)化為電能的有效裝置,與傳統(tǒng)電池相比,具有能量轉(zhuǎn)化率高、生物相容性好、原料來源廣等優(yōu)勢,是一種真正意義上的綠色電池,有望解決環(huán)境與能源的危機。目前需要解決的問題是讓酶高效固定在電極上且實現(xiàn)電子的有效傳遞。本文以三維多孔泡沫碳(3 D-PCF)或超短摻氮碳納管(N-STCs)為電極載體材料,進行表面修飾以此來固定酶和中間體,并通過多種表征方法系統(tǒng)的研究了酶電極和燃料電池的性能。本文具體研究內(nèi)容如下：(1)以三維多孔泡沫碳(3D-PCF)為電極載體材料,通過氨基二茂鐵的重氮化實現(xiàn)了中間體在3D-PCF上的固定,得到Fc@3D-PCF,然后利用戊二醛將殼聚糖(CS)和葡萄糖氧化酶(GOD)共價交聯(lián)到Fc@3D-PCF上,制備GOD-CS/Fc@3D-PCF電極材料。構(gòu)建以GOD-CS/Fc@3D-PCF/GC為陽極和E-TEKPt/C電極為陰極的葡萄糖/O2 EBFC。所構(gòu)建的酶生物燃料電池的開路電位(Voc)和短路電流(Isc)分別為0.77 V和0.38 mA cm-2,在0.50 V時,獲取最大的功率輸出密度為64.3μW cm-2。(2)基于已制備的固定了氨基二茂鐵的材料Fc@3D-PCF,然后利用醛基二茂鐵的醛基和GOD的氨基通過席夫堿共價鏈接得到Fc-GOD,再通過GA交聯(lián)Fc-GOD附著在Fc@3D-PCF上制得GOD-Fc/Fc@3D-PCF電極材料,構(gòu)建了以GOD-CS/Fc@3D-PCF/GC為陽極和E-TEKPt/C電極為陰極的葡萄糖/O2 EBFC。所構(gòu)建的酶生物燃料電池的開路電位(Voc)和短路電流(Isc)分別為0.88 V和0.58 mAcm-2,在0.40 V時,獲取最大的功率輸出密度為116.4μW cm-2,較之前固定單-中間體各項性能有明顯的提升(3)利用自制摻氮碳納米管(N-STC)為載體材料,通過負載納米金并共同固定中間體和葡萄萄糖氧化酶的方法,制備了EBFC陽極GOD/Fc-CYS-Au/N-STC,并構(gòu)建了以GOD/Fc-CYS-Au/N-STC電極為陽極和E-TEK Pt/C電極為陰極的葡萄糖/O2EBFC,所構(gòu)建的酶生物燃料電池的開路電位(Voc)和短路電流(Isc)分別為0.71 V和01.78 mA cm-2,在0.21 V時,獲取最大的功率輸出密度為83.6μW cm-2。
[Abstract]:Enzyme biofuel cell (EBFC) is an effective device which uses biomass fuel as raw material and enzyme as catalyst to convert chemical energy into electric energy. Because of its advantages of good biocompatibility and wide sources of raw materials, it is a kind of real green battery. It is expected to solve the environmental and energy crisis. At present, the problem that needs to be solved is that the enzyme can be efficiently immobilized on the electrode and the effective transfer of electrons can be realized. In this paper, the three-dimensional porous carbon foam 3D-PCF is used to solve the problem. Or ultrashort nitrogen-doped carbon nanotubes (N-STCs) are used as electrode carrier materials. To immobilize enzymes and intermediates by surface modification. The performance of enzyme electrode and fuel cell was systematically studied by various characterization methods. The main contents of this paper are as follows: 1) Three-dimensional porous carbon foam 3D-PCF) was used as electrode carrier. The intermediate was immobilized on 3D-PCF by diazotization of ferrocene and Fc@3D-PCF was obtained. Then glutaraldehyde was used to cross-link chitosan (CSS) and glucose oxidase (GODs) to Fc@3D-PCF. Preparation of GOD-CS/Fc@3D-PCF electrode Materials. Fabrication of glucose / O _ 2 with GOD-CS/Fc@3D-PCF/GC as anode and E-TEKPt / C electrode as cathode. Open-circuit potential of enzyme biofuel cell constructed by EBFC. Vocabulary and short circuit current were 0.77V and 0.38mA cm-2, respectively. At 0.50 V, the maximum power output density of 64.3 渭 W cm-2.2) was obtained based on the immobilized material Fc@3D-PCF. Then Fc-GOD was obtained by covalent linking of aldehyde group of aldehyde ferrocene and amino group of GOD by Schiff base. Then the GOD-Fc/Fc@3D-PCF electrode material was prepared by GA crosslinking Fc-GOD attached to Fc@3D-PCF. Using GOD-CS/Fc@3D-PCF/GC as anode and E-TEKPT / C electrode as cathode, glucose / O _ 2 / O _ 2 was constructed. Open-circuit potential of enzyme biofuel cell constructed by EBFC. Vocabulary and short circuit current were 0.88V and 0.58 mAcm-2, respectively. At 0.40 V, the maximum power output density is 116.4 渭 W cm-2. Compared with the previous fixed mono-intermediates, the properties of the mono-intermediates were improved obviously. The N-STC / NT-doped carbon nanotubes (NCNTs) were used as the support materials. EBFC anode GOD/Fc-CYS-Au/N-STC was prepared by loading nano-gold and fixing intermediate and grape glucose oxidase. Glucose / O2 EBFC with GOD/Fc-CYS-Au/N-STC electrode as anode and E-TEK Pt/C electrode as cathode was constructed. The open circuit potential (Vocg) and short circuit current (Iscc) of the constructed enzyme biofuel cell were 0.71 V and 0.78 Ma / cm ~ (-2), respectively, at 0.21 V. The maximum power output density is 83.6 渭 W cm-2.
【學(xué)位授予單位】：湖南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：TQ127.11;TM911.4
，

本文編號：1382432