化學(xué)發(fā)光（Chemiluminescence,CL）是化學(xué)反應(yīng)過(guò)程中的一種光輻射現(xiàn)象。溶液相中的化學(xué)發(fā)光反應(yīng)大多是基于氧化還原反應(yīng)。在化學(xué)反應(yīng)過(guò)程中,產(chǎn)物從反應(yīng)中獲得能量,從基態(tài)躍遷到激發(fā)態(tài),分子從激發(fā)態(tài)回到基態(tài)的過(guò)程中以光子的形式釋放能量,即化學(xué)發(fā)光。化學(xué)發(fā)光分析法是一種分子發(fā)光光譜分析方法,主要是基于待測(cè)化合物的濃度與在一定條件下的CL強(qiáng)度之間的線性關(guān)系進(jìn)行定量分析。常見(jiàn)的化學(xué)發(fā)光分析體系有過(guò)氧草酸酯化學(xué)發(fā)光（Peroxyoxalate Chemiluminescence,PO-CL）體系、光澤精（Lucigenin）化學(xué)發(fā)光體系、聯(lián)吡啶釕電致化學(xué)發(fā)光體系、魯米諾（Luminol）化學(xué)發(fā)光體系等。其中,魯米諾化學(xué)發(fā)光體系是最常用的一種化學(xué)發(fā)光體系,已被廣泛應(yīng)用于藥物分析、環(huán)境分析和生物分析等領(lǐng)域測(cè)定不同的目標(biāo)分析物,包括金屬離子、葡萄糖、維生素、蛋白質(zhì)、核酸、環(huán)境污染物等。然而,在堿性條件下,魯米諾的自身化學(xué)發(fā)光反應(yīng)強(qiáng)度非常弱。因此,發(fā)展有效的催化劑和增強(qiáng)劑對(duì)新型發(fā)光體系的發(fā)展及應(yīng)用具有重要意義。魯米諾體系常見(jiàn)的催化劑包括金屬離子、天然酶、納米材料等。而增強(qiáng)劑則主要用于過(guò)氧化物酶（... 

【文章來(lái)源】：天津大學(xué)天津市 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：98 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
中文摘要
ABSTRACT
Chapter1 Literature review
    1.1.Chemiluminescence and chemiluminescence analytical methods
    1.2.Common chemiluminescence analytical system
        1.2.1.Peroxyoxalate chemiluminescence system
        1.2.2.Acridinium salts chemiluminescence system
        1.2.3.Tris(2,2'-bipyridyl)ruthenium(II)chemiluminescence system
        1.2.4.Luminol chemiluminescence system
    1.3.Catalysts for luminol-H_2O_2 chemiluminescence system
        1.3.1.Metal ions(M~(n+))
        1.3.2.Peroxidase
        1.3.3.Nanomaterials(as mimetic peroxidase)
    1.4.Enhancers for luminol chemiluminescence system
        1.4.1.Fluorescein,naphthols,and hexahydroxybenzothiazole
        1.4.2.Substituted phenol derivatives
        1.4.3.Lophine derivatives and analogues
        1.4.4.Substituted boronic acid derivatives
        1.4.5.Water-soluble macromolecules and surfactants
        1.4.6.Co-enhancers
    1.5.The origin of my research work
Chapter2 Enhancement effect of p-iodophenol on gold nanoparticle-catalyzed chemiluminescence
    2.1.Introduction
    2.2.Experimental
        2.2.1.Apparatus and reagents
        2.2.2.Synthesis and characterization of Au NPs
        2.2.3.Procedure of CL measurement
    2.3.Results and discussions
        2.3.1.Characterization of Au NPs
        2.3.2.The enhancement effect of PIP on the luminol-H_2O_2-Au NPs CL system
        2.3.3.The enhancement effect of nine enhancers on the luminol-O_2-Au NPs CL system
        2.3.4.Kinetic curve of PIP-enhanced luminol-O_2-Au NPs CL system
        2.3.5.Optimization of the reaction conditions for the PIP-enhanced luminol-O_2-Au NPs CL system
        2.3.6.Catalytic activity of Au NPs with different diameters
        2.3.7.Selectivity of the proposed CL system
        2.3.8.Possible mechanism of the PIP-enhanced CL system
        2.3.9.Analytical applications of the proposed CL system
    2.4.Conclusions
Chapter3 Application of p-iodophenol-enhanced chemiluminescence reaction to immunoassay
    3.1.Introduction
    3.2.Experimental
        3.2.1.Apparatus and reagents
        3.2.2.Synthesis and characterization of Au NPs
        3.2.3.Preparation of Au NPs-labeled goat-anti-rabbit IgG secondary antibody..
        3.2.4.Assay procedure for CL detection of rabbit IgG
    3.3.Results and discussions
        3.3.1.Characterization of Au NPs
        3.3.2.Scheme for the detection of rabbit IgG
        3.3.3.Optimization of the assay conditions
        3.3.4.Assay performance for detection of rabbit IgG
        3.3.5.Selectivity of the proposed CL immunoassay system
        3.3.6.Analysis of rabbit IgG in the serum sample
    3.4.Conclusions
Chapter4 Conclusions and outlook
References
Published papers and scientific research
Acknowledgements


【參考文獻(xiàn)】：
期刊論文
[1]苯酚和苯胺類化合物對(duì)光澤精化學(xué)發(fā)光的增強(qiáng)和抑制作用[J]. 石明娟,Aung Myint,董永平,崔華.  光譜學(xué)與光譜分析. 2007(10)
[2]金納米粒子表面自組裝巰基十一烷醇單分子層體系的制備及其光散射特性研究[J]. 尹洪宗,劉輝,李園園,何錫文,陳朗星,李文友.  化學(xué)學(xué)報(bào). 2005(08)



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