【摘要】：本文采用層接層自組裝的方法，用石墨烯、金納米粒子或三聯(lián)吡啶釕[Ru(bpy)3]修飾兩種釩取代型多酸，分別在氧化銦錫玻璃（ITO）、石英和硅片上制備了兩種復(fù)合薄膜：{PEI/[P2W16V2-Au/PDDA-rGO]8/P2W16V2}和{PEI/[PMo9V3/Ru(bpy)3/PSS/PDDA-rGO]5/PMo9V3}。 利用紫外-可見吸收光譜（UV-vis）對(duì)薄膜的增長進(jìn)行監(jiān)測，結(jié)果表明兩種復(fù)合薄膜是均勻穩(wěn)定增長的。利用原子力顯微鏡（AFM）和掃描電鏡（SEM）對(duì)復(fù)合薄膜的表面形貌和粗糙度進(jìn)行表征，結(jié)果顯示薄膜表面呈微褶皺狀，納米粒子均勻分散在上面。通過X-射線光電子能譜（XPS）研究復(fù)合薄膜的組成，結(jié)果顯示多酸、石墨烯、金納米粒子或[Ru(bpy)3]均成功的沉積到薄膜上。采用安培計(jì)時(shí)法和循環(huán)伏安法（CV）研究了復(fù)合薄膜的電化學(xué)性質(zhì)和傳感尿酸（UA）或抗壞血酸（AA）的性質(zhì)。電化學(xué)實(shí)驗(yàn)結(jié)果表明復(fù)合膜{PEI/[P2W16V2-Au/PDDA-rGO]8/P2W16V2}在pH=7的生理?xiàng)l件下對(duì)UA有良好的電化學(xué)活性，催化電位為0.43V，催化電流與UA濃度成良好的線性關(guān)系，檢測范圍是2.5×10-7~1.025×10-4M，，檢測限是1.4×10-7M，靈敏度為0.24μM/μA。同時(shí)，該復(fù)合薄膜展示了良好的穩(wěn)定性和重現(xiàn)性。實(shí)驗(yàn)中將其用于尿樣中UA的檢測，得到的平均回收率是100.4%，在催化電位0.43V下，可能的潛在干擾物質(zhì)對(duì)其幾乎沒有響應(yīng)。復(fù)合薄膜{PEI/[PMo9V3/Ru(bpy)3/PSS/PDDA-rGO]5/PMo9V3}在pH=6.5的PBS緩沖溶液可以用于檢測AA，催化電位為0.15V。時(shí)間電流的線性分析得到復(fù)合薄膜對(duì)AA的檢測范圍是7.5×10-7~2.07×10-4M，檢測限是1.0×10-7M，靈敏度為0.16μM/μA。該AA傳感器對(duì)其他的物質(zhì)具有良好的抗干擾性。在真實(shí)樣品的檢測中，該AA傳感器取得了令人滿意的結(jié)果。
[Abstract]:In this paper, two vanadium substituted polyacids were modified by graphene, gold nanoparticles or tripyridine ruthenium [Ru (bpy) 3] by lamination self-assembly in indium tin oxide glass (ITO), respectively. Two kinds of composite thin films were prepared on quartz and silicon wafer: {PEI/ [P2W16V2-Au/PDDA-rGO] 8/P2W16V2} and {PEI/ [PMo9V3/Ru (bpy) 3/PSS/PDDA-rGO] 5/PMo9V3}. The growth of the films was monitored by UV-vis absorption spectroscopy (UV-vis). The results show that the two kinds of composite films grow uniformly and steadily. The surface morphology and roughness of the composite films were characterized by atomic force microscope (AFM) and scanning electron microscope (SEM). The results showed that the surface of the films was micropleated and the nanoparticles were uniformly dispersed on them. The composition of the composite films was studied by X-ray photoelectron spectroscopy (XPS). The results showed that polyacid, graphene, gold nanoparticles or [Ru (bpy) 3] were successfully deposited on the films. The electrochemical properties of the composite films and the sensing properties of uric acid (UA) or ascorbic acid (AA) were studied by amperometric chronograph and cyclic Voltammetric method (CV). The results of electrochemical experiments show that the composite membrane {PEI/ [P2W16V2-Au/PDDA-rGO] 8/P2W16V2} has good electrochemical activity against UA under the physiological conditions of pH=7, the catalytic potential is 0.43V, and the catalytic current has a good linear relationship with the concentration of UA. The detection range is 2.5 脳 10 鈮

本文編號(hào)：2483014