可吸收引導(dǎo)骨再生膜力學(xué)性能研究
發(fā)布時間：2019-04-24 03:48
【摘要】：引導(dǎo)骨再生(Guided Bone Regeneration, GBR)是近年來新誕生的一種組織工程學(xué)方法。電紡絲是簡捷連續(xù)制備一維納米纖維技術(shù),脂肪族聚酯已經(jīng)通過靜電紡絲成功地制成納米纖維,并且在引導(dǎo)骨再生研究領(lǐng)域展現(xiàn)出廣闊前景。然而電紡納米纖維膜較低的力學(xué)強度成了難解的問題。為此,在本文中,我們合成了各種星型已內(nèi)酯(star-shaped PCL),探討其相關(guān)性能；將其作為增強劑與聚丙交酯共混紡絲,研究共混纖維膜的力學(xué)和相關(guān)性能的變化；還將SAPCL、改性接枝羥磷灰石(HA-g-PLLA)和表面活性劑PF-108聯(lián)合,研究協(xié)同作用對纖維膜的力學(xué)及相關(guān)性能的影響；在此基礎(chǔ)上,利用溶劑汽化交聯(lián)進(jìn)一步增強纖維膜。四組分復(fù)合納米纖維膜具有良好的親水性和力學(xué)性能適于引導(dǎo)骨再生修復(fù)要求。1.成功合成了2,3,4和6臂的SAPCL,通過H'-NMR、GPC、XRD、DSC對材料進(jìn)行表征,結(jié)果表明相同分子量的PCLs隨著臂數(shù)的增加,支臂長度逐漸變小,短的支臂更受束,它限制了多臂星型PCLs的每條臂的流動性,致使PCL的結(jié)晶度變小。2.通過靜電紡絲方法成功制備了多臂PCL與聚乳酸(PLLA)共混納米纖維膜。利用SEM觀察納米纖維的形貌,DSC研究了共混纖維膜的熱學(xué)性質(zhì),并通過拉伸測試研究了SAPCL的臂數(shù)、分子量、混紡比等對納米纖維膜的力學(xué)性能的影響,當(dāng)SAPCL混入量為20wt%時,共混纖維膜的拉伸強度達(dá)到最大值,并且共混納米纖維膜無細(xì)胞毒性。3.利用靜電紡絲方法成功制備了PLGA/PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108四組分復(fù)合纖維膜。通過SEM、TEM觀察了納米纖維的形貌和無機粒子在纖維中分布,紅外分析說明四組分只是物理共混,DSC研究了四組分纖維膜的熱學(xué)性質(zhì),通過拉伸測試研究了不同組分對纖維膜力學(xué)性能的影響,四組分纖維膜的拉伸強度遠(yuǎn)大純PLGA纖維膜,測試分析了不同組份纖維親水性、降解性和生物活性的差異。其中加入PF108后,PLGA中的-CH3和PF-108中的-CH3疏水性相互作用限制EO融入PLGA聚合物鏈,迫使它們突向表面,使共混纖維膜的親水性增加。MTT測試表明所獲四組分纖維膜無細(xì)胞毒性。4.基于PLGA和PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108納米纖維膜,探討溶劑汽化交聯(lián)進(jìn)一步增強納米纖維膜的力學(xué)性能。氮氣流速、交聯(lián)時間和交聯(lián)溶劑比例是影響交聯(lián)的重要因素,通過正交實驗表明優(yōu)化的工藝條件,并且用SEM觀察交聯(lián)前后的形貌變化,MTT測試表明交聯(lián)納米纖維膜無細(xì)胞毒性。
[Abstract]:Guided bone regeneration (Guided Bone Regeneration, GBR) is a new tissue engineering method in recent years. Electrospinning is a simple and continuous one-dimensional nanofiber preparation technology. Aliphatic polyester has been successfully made into nanofibers by electrospinning, and has shown a broad prospect in the research field of guided bone regeneration. However, the low mechanical strength of electrospun nanofiber film becomes a difficult problem. Therefore, in this paper, we synthesized various star caprolactone (star-shaped PCL), used it as reinforcing agent and polylactide blend spinning to study the change of mechanical and related properties of blend fiber membrane. SAPCL, modified hydroxyapatite (HA-g-PLLA) and surfactant PF-108 were combined to study the influence of synergistic action on the mechanical and related properties of the fiber membrane, and on the basis of this, the fiber membrane was further strengthened by solvent vaporization cross-linking. The four-component composite nanofiber membrane has good hydrophilicity and mechanical properties suitable for guiding bone regeneration and repair. 1. The SAPCL, of 2,3,4 and 6 arms were synthesized and characterized by PCLs, GPC, XRD and DSC. The results showed that the length of the arms of the same molecular weight became smaller with the increase of the number of arms, and the shorter arms were more affected by the beam, and the results showed that the PCLs of the same molecular weight gradually decreased with the increase of the number of arms. It limits the mobility of each arm of the multi-arm star-shaped PCLs and reduces the crystallinity of PCL. Multi-arm PCL / polylactic acid (PLLA) blend nanofiber membranes were successfully prepared by electrospinning. The morphology of nanofibers was observed by SEM, the thermal properties of blend fiber membranes were studied by DSC, and the effects of the number of arms, molecular weight and blending ratio of SAPCL on the mechanical properties of nano-fiber membranes were studied by tensile test. When the mixing amount of SAPCL was 20 wt%, the mechanical properties of nano-fiber membranes were studied. The tensile strength of the blend fiber membrane reaches the maximum and the blend nanofiber membrane has no cytotoxicity. 3. PLGA/20%SAPCL/5%HA-g-PLLA/1%PF- 108 four-component composite fiber membrane was successfully prepared by electrospinning. The morphology of nano-fiber and the distribution of inorganic particles in the fiber were observed by SEM,TEM. The infrared analysis showed that the four components were only physical blend. The thermal properties of the four-component fiber membrane were studied by DSC. The effects of different components on the mechanical properties of fiber membrane were studied by tensile test. The tensile strength of four-component fiber membrane was much larger than that of pure PLGA fiber membrane. The differences of hydrophilicity, degradability and biological activity of four-component fiber membrane were tested and analyzed. With the addition of PF108, the hydrophobic interaction between-CH3 in PLGA and-CH3 in PF-108 restricts EO's inclusion in the PLGA polymer chain, forcing them to protrude to the surface. The hydrophilicity of the blend fiber membrane was increased. MTT test showed that the four components of the blend fiber membrane had no cytotoxicity. 4. Based on PLGA and PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108 nano-fiber membrane, the mechanical properties of nano-fiber membrane were further enhanced by solvent vaporization cross-linking. Nitrogen flow rate, cross-linking time and cross-linking solvent ratio are important factors to influence cross-linking. The optimum technological conditions are shown by orthogonal experiment, and the morphology changes before and after cross-linking are observed by SEM. MTT test shows that the cross-linked nano-fiber membrane has no cytotoxicity.
【學(xué)位授予單位】：長春工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.2
本文編號：2464079
[Abstract]:Guided bone regeneration (Guided Bone Regeneration, GBR) is a new tissue engineering method in recent years. Electrospinning is a simple and continuous one-dimensional nanofiber preparation technology. Aliphatic polyester has been successfully made into nanofibers by electrospinning, and has shown a broad prospect in the research field of guided bone regeneration. However, the low mechanical strength of electrospun nanofiber film becomes a difficult problem. Therefore, in this paper, we synthesized various star caprolactone (star-shaped PCL), used it as reinforcing agent and polylactide blend spinning to study the change of mechanical and related properties of blend fiber membrane. SAPCL, modified hydroxyapatite (HA-g-PLLA) and surfactant PF-108 were combined to study the influence of synergistic action on the mechanical and related properties of the fiber membrane, and on the basis of this, the fiber membrane was further strengthened by solvent vaporization cross-linking. The four-component composite nanofiber membrane has good hydrophilicity and mechanical properties suitable for guiding bone regeneration and repair. 1. The SAPCL, of 2,3,4 and 6 arms were synthesized and characterized by PCLs, GPC, XRD and DSC. The results showed that the length of the arms of the same molecular weight became smaller with the increase of the number of arms, and the shorter arms were more affected by the beam, and the results showed that the PCLs of the same molecular weight gradually decreased with the increase of the number of arms. It limits the mobility of each arm of the multi-arm star-shaped PCLs and reduces the crystallinity of PCL. Multi-arm PCL / polylactic acid (PLLA) blend nanofiber membranes were successfully prepared by electrospinning. The morphology of nanofibers was observed by SEM, the thermal properties of blend fiber membranes were studied by DSC, and the effects of the number of arms, molecular weight and blending ratio of SAPCL on the mechanical properties of nano-fiber membranes were studied by tensile test. When the mixing amount of SAPCL was 20 wt%, the mechanical properties of nano-fiber membranes were studied. The tensile strength of the blend fiber membrane reaches the maximum and the blend nanofiber membrane has no cytotoxicity. 3. PLGA/20%SAPCL/5%HA-g-PLLA/1%PF- 108 four-component composite fiber membrane was successfully prepared by electrospinning. The morphology of nano-fiber and the distribution of inorganic particles in the fiber were observed by SEM,TEM. The infrared analysis showed that the four components were only physical blend. The thermal properties of the four-component fiber membrane were studied by DSC. The effects of different components on the mechanical properties of fiber membrane were studied by tensile test. The tensile strength of four-component fiber membrane was much larger than that of pure PLGA fiber membrane. The differences of hydrophilicity, degradability and biological activity of four-component fiber membrane were tested and analyzed. With the addition of PF108, the hydrophobic interaction between-CH3 in PLGA and-CH3 in PF-108 restricts EO's inclusion in the PLGA polymer chain, forcing them to protrude to the surface. The hydrophilicity of the blend fiber membrane was increased. MTT test showed that the four components of the blend fiber membrane had no cytotoxicity. 4. Based on PLGA and PLGA/20%SAPCL/5%HA-g-PLLA/1%PF-108 nano-fiber membrane, the mechanical properties of nano-fiber membrane were further enhanced by solvent vaporization cross-linking. Nitrogen flow rate, cross-linking time and cross-linking solvent ratio are important factors to influence cross-linking. The optimum technological conditions are shown by orthogonal experiment, and the morphology changes before and after cross-linking are observed by SEM. MTT test shows that the cross-linked nano-fiber membrane has no cytotoxicity.
【學(xué)位授予單位】：長春工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：TB383.2
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 張鵬云;張鵬云;張鵬云;張建松;張建松;張建松;徐曉紅;徐曉紅;莫秀梅;莫秀梅;莫秀梅;莫秀梅;何創(chuàng)龍;何創(chuàng)龍;;生物交聯(lián)劑京尼平對靜電紡明膠納米纖維膜改性的影響[J];中國組織工程研究與臨床康復(fù);2009年08期
，本文編號：2464079
本文鏈接：http://sikaile.net/kejilunwen/cailiaohuaxuelunwen/2464079.html
最近更新
教材專著
