【摘要】：化學(xué)發(fā)光分析不需要外界光源,具有檢測靈敏度高、線性范圍寬、儀器簡單經(jīng)濟(jì)、分析速度快等優(yōu)點(diǎn),是光譜分析中的研究熱點(diǎn)之一。由于大部分化學(xué)發(fā)光反應(yīng)的反應(yīng)速率慢、量子產(chǎn)率低,尋找合適的催化劑來提升化學(xué)發(fā)光反應(yīng)的發(fā)光強(qiáng)度一直是化學(xué)發(fā)光研究領(lǐng)域的熱門課題。作為一種綠色環(huán)保的無機(jī)納米功能材料,海泡石以其獨(dú)特的多孔結(jié)構(gòu)和纖維形貌受到了研究者們的廣泛關(guān)注,被廣泛地應(yīng)用于催化、吸附、功能材料等領(lǐng)域,但是在化學(xué)發(fā)光方面的應(yīng)用卻未見報(bào)道。本論文首次將海泡石納米材料與化學(xué)發(fā)光分析法相結(jié)合,研究了海泡石對魯米諾化學(xué)發(fā)光體系的影響,建立了基于海泡石-魯米諾-過氧化氫化學(xué)發(fā)光新體系的化學(xué)發(fā)光分析法,結(jié)合流動注射分析技術(shù)實(shí)現(xiàn)了對環(huán)境、食品樣品中微量成分的高靈敏度測定,拓寬了海泡石的應(yīng)用范圍,在開發(fā)新型、高效化學(xué)發(fā)光體系催化劑方面做出了積極、有益的探索。本文主要取得了以下研究成果：1.開發(fā)了一種新型高效的魯米諾-過氧化氫化學(xué)發(fā)光體系的催化劑—海泡石,考察了海泡石的pH值、反應(yīng)溫度和時(shí)間等因素對魯米諾-過氧化氫化學(xué)發(fā)光體系的影響。研究結(jié)果表明,在pH值為1.0,100℃下反應(yīng)4h得到的海泡石晶形規(guī)整、形貌均一,催化效果良好,增敏倍數(shù)近400倍。2.基于海泡石催化魯米諾-過氧化氫化學(xué)發(fā)光體系,建立了一種快速準(zhǔn)確測定過氧化氫含量的化學(xué)發(fā)光方法。該分析方法的線性范圍為1.0×10.8～8.0à10-6 mol·L-1,檢出限(S/N=3)為8.8×10.9 mol.L-1。該方法成功應(yīng)用于自來水和雨水樣品中過氧化氫含量的測定,加標(biāo)回收率在98～104%之間。3.基于海泡石催化魯米諾-過氧化氫化學(xué)發(fā)光體系,設(shè)計(jì)并開發(fā)了一種新型的化學(xué)發(fā)光傳感器,該傳感器測定葡萄糖的線性范圍為7.5×10.6～ 2.0×10-3 mol.L-1，檢出限(S/N=3)為3.2×10.6 mOl.L-1。采用該傳感器測定飲料中葡萄糖的濃度,得到了令人滿意的分析結(jié)果。
[Abstract]:Chemiluminescence analysis has the advantages of high detection sensitivity, wide linear range, simple and economical instrument, fast analytical speed and so on. It is one of the research hotspots in spectral analysis. Because the reaction rate of most chemiluminescence reactions is slow and the quantum yield is low, it has been a hot topic in the field of chemiluminescence research to find suitable catalysts to enhance the luminescence intensity of chemiluminescence reactions. As a kind of green inorganic nano-functional materials, sepiolite has been widely concerned by researchers for its unique porous structure and fiber morphology, and has been widely used in the fields of catalysis, adsorption, functional materials and so on. However, applications in chemiluminescence have not been reported. In this paper, the effect of sepiolite on the luminol chemiluminescence system was studied by combining the sepiolite nanomaterials with chemiluminescence analysis for the first time. A new chemiluminescence analysis method based on sepiolite-Lumino-hydrogen peroxide chemiluminescence system was established. A high sensitivity method for the determination of trace components in food and environment samples was achieved by flow injection analysis. The application range of sepiolite has been widened and active and beneficial exploration has been made in the development of new and high efficient chemiluminescence system catalysts. The main achievements of this paper are as follows: 1. Sepiolite, a new and efficient catalyst for luminol hydrogen peroxide chemiluminescence system, was developed. The effects of pH value, reaction temperature and reaction time on the luminol hydrogen peroxide chemiluminescence system were investigated. The results show that the sepiolite prepared by reaction at 1.0100 鈩，

本文編號：2398101