FDTD仿真金屬納米顆粒LSPR特性及在生物光子學上的應(yīng)用研究
發(fā)布時間：2018-12-19 11:09
【摘要】：近年來,納米科學領(lǐng)域的蓬勃發(fā)展使得金屬納米顆粒的制備表征取得了矚目的成就,其具有的一些獨特的光學、電學特性也逐漸呈現(xiàn)在世人面前。發(fā)展至今,后續(xù)的應(yīng)用研究已經(jīng)在物理、化學、生命醫(yī)學等領(lǐng)域得到了廣泛的開展。目前,金屬納米顆粒不僅成為了進行光與物質(zhì)相互作用研究最好的平臺,而且為生命科學領(lǐng)域提供了重要的研究方法和檢測途徑。因此,研究關(guān)于金屬納米結(jié)構(gòu)獨具魅力的特性及其與生物分子間的相互作用,對于研究開發(fā)性能穩(wěn)定、效果良好的近場成像技術(shù)以及進行生物分子空間分辨特性檢測等工作的開展具有非常積極而重大的意義�；诮饘偌{米顆粒在生命科學研究中的重要性,結(jié)合本課題組的研究工作基礎(chǔ),本論文以金屬納米顆粒為研究對象,使用時域有限差分法(FDTD)仿真模擬研究了金屬納米顆粒的光學特性,及其在生物光子學領(lǐng)域的應(yīng)用。通過利用局域表面等離子體激元共振(LSPR)相互作用機制設(shè)計了近場LSPR信號放大器,并分析了在生物光子學領(lǐng)域的潛在應(yīng)用。在金屬納米顆粒與熒光分子相互作用機制的基礎(chǔ)上,拓展了金屬納米顆粒在熒光分子空間分辨特性探測上的應(yīng)用。不僅對金屬納米顆粒的LSPR特性進行了深入的研究討論,同時對在生命科學領(lǐng)域的應(yīng)用進行了優(yōu)化和開拓。本論文主要的研究成果如下：1、系統(tǒng)研究了金屬納米顆粒二聚體的LSPR特性�？疾炝薒SPR耦合相互作用對二聚體金屬結(jié)構(gòu)局域電場分布的影響。相比較單顆粒局域電場分布,二聚體納米結(jié)構(gòu)具有更強的局域電場增強能力。使用金屬自由電荷分布及電荷相互作用機制深入分析了二聚體納米結(jié)構(gòu)的LSPR特性,這對我們接下來金屬納米顆粒二聚體放大器的設(shè)計和優(yōu)化提供了理論指導；2、設(shè)計了一種基于二聚體結(jié)構(gòu)的LSPR放大器,實現(xiàn)了在局域范圍內(nèi)的電場放大,這對一些微弱信號的增強探測有著積極的作用。通過在二聚體外部包裹增益材料的方法來克服金屬納米顆粒的吸收損耗,極大的增強了二聚體結(jié)構(gòu)周圍的局域電場。研究二聚體納米粒子間距d的大小對局域電場分布的影響,選擇合適的間距情況下考察了LSPR放大器的近場增強能力,探討了它的應(yīng)用前景.相關(guān)結(jié)果使用時域有限差分法(FDTD)計算得到.通過調(diào)節(jié)二聚體納米粒子的間距,實現(xiàn)特定的信號增強。3、研究了利用金屬納米顆粒附近分子的熒光發(fā)射原理,進行生物分子間的相互作用研究的可行性。拓寬了探測表征手段,除了常用的熒光強度等參數(shù),我們還利用量子效率、熒光壽命等參數(shù)來進行相關(guān)表征。通過靈活多樣的探測手段,此方法可以應(yīng)用于蛋白、DNA、抗體-抗原相關(guān)的結(jié)合作用研究。并且可以更加靈敏地進行分子間結(jié)合作用研究。使用FDTD仿真計算了處于金屬納米顆粒附近熒光分子的發(fā)射特性,探討了能量交換的機理。通過對比不同條件下處于金屬納米顆粒附近熒光分子的發(fā)射特性,根據(jù)量子效率和熒光壽命來探測熒光分子的空間分辨特性,從而研究生物分子間的結(jié)合作用。
[Abstract]:In recent years, the development of the nano-science field has made the preparation and characterization of the metal nano-particles remarkable achievement, and has some unique optical and electrical characteristics. So far, the follow-up research has been carried out in the fields of physics, chemistry, life medicine and so on. At present, the metal nanoparticles not only become the best platform for the interaction of light and materials, but also provide important research methods and methods for the field of life science. Therefore, the study on the characteristics of the unique charm of the metal nano-structure and its interaction with the biological molecules, the research and development performance is stable, It is of great significance to carry out the near-field imaging technology with good effect and to carry out the detection of the biological molecule space resolution property. Based on the importance of metal nanoparticles in the research of life science, and based on the research work of the research group, the optical properties of the metal nanoparticles were studied by using the time-domain finite difference method (FDTD) simulation. and the application thereof in the field of biological photonics. The near field LSPR signal amplifier is designed by the local surface plasmon resonance (LSPR) interaction mechanism, and the potential application in the field of biological photonics is analyzed. On the basis of the interaction mechanism between the metal nanoparticles and the fluorescent molecules, the application of the metal nanoparticles in the detection of the space-resolved properties of the fluorescent molecules is expanded. Not only the LSPR characteristics of the metal nanoparticles have been studied in depth, but also the application in the field of life science is optimized and developed. The main results of this thesis are as follows: 1. The LSPR characteristic of the metal nanoparticle dimer is studied. The effect of the LSPR coupling interaction on the local electric field distribution of the dimer metal structure was investigated. Compared with the single-particle local electric field distribution, the dimeric nanostructures have a stronger local electric field enhancement capability. The LSPR characteristic of the dimer nano-structure is deeply analyzed by using the metal free charge distribution and the charge interaction mechanism, which provides the theoretical guidance for the design and optimization of the next metal nano-particle dimer amplifier; and 2, an LSPR amplifier based on the dimer structure is designed, The electric field amplification in the local area is realized, which has a positive effect on the enhancement detection of some weak signals. by wrapping the gain material outside the dimer, the absorption loss of the metal nanoparticles is overcome, and the local electric field around the dimer structure is greatly enhanced. The influence of the size of the nano-particle spacing d on the distribution of the local electric field is studied, and the near field enhancement of the LSPR amplifier is investigated under the condition of proper spacing, and its application prospect is discussed. The correlation results are calculated using the time-domain finite difference method (FDTD). By adjusting the spacing of the dimer nanoparticles, the specific signal enhancement was achieved. 3. The fluorescence emission principle of the molecules near the metal nanoparticles was used to study the interaction between the biomolecules. In addition to the commonly used parameters such as fluorescence intensity, we also use the parameters of quantum efficiency, fluorescence lifetime and so on to make relevant representation. The method can be applied to the binding action of protein, DNA and antibody-antigen. and the intermolecular binding effect can be more sensitively carried out. The emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles were calculated by the FDTD method, and the mechanism of energy exchange was discussed. by comparing the emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles under different conditions, the spatial resolution characteristics of the fluorescent molecules are detected according to the quantum efficiency and the fluorescence lifetime, and the binding action between the biomolecules is studied.
【學位授予單位】：華東師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1;O657.3
[Abstract]:In recent years, the development of the nano-science field has made the preparation and characterization of the metal nano-particles remarkable achievement, and has some unique optical and electrical characteristics. So far, the follow-up research has been carried out in the fields of physics, chemistry, life medicine and so on. At present, the metal nanoparticles not only become the best platform for the interaction of light and materials, but also provide important research methods and methods for the field of life science. Therefore, the study on the characteristics of the unique charm of the metal nano-structure and its interaction with the biological molecules, the research and development performance is stable, It is of great significance to carry out the near-field imaging technology with good effect and to carry out the detection of the biological molecule space resolution property. Based on the importance of metal nanoparticles in the research of life science, and based on the research work of the research group, the optical properties of the metal nanoparticles were studied by using the time-domain finite difference method (FDTD) simulation. and the application thereof in the field of biological photonics. The near field LSPR signal amplifier is designed by the local surface plasmon resonance (LSPR) interaction mechanism, and the potential application in the field of biological photonics is analyzed. On the basis of the interaction mechanism between the metal nanoparticles and the fluorescent molecules, the application of the metal nanoparticles in the detection of the space-resolved properties of the fluorescent molecules is expanded. Not only the LSPR characteristics of the metal nanoparticles have been studied in depth, but also the application in the field of life science is optimized and developed. The main results of this thesis are as follows: 1. The LSPR characteristic of the metal nanoparticle dimer is studied. The effect of the LSPR coupling interaction on the local electric field distribution of the dimer metal structure was investigated. Compared with the single-particle local electric field distribution, the dimeric nanostructures have a stronger local electric field enhancement capability. The LSPR characteristic of the dimer nano-structure is deeply analyzed by using the metal free charge distribution and the charge interaction mechanism, which provides the theoretical guidance for the design and optimization of the next metal nano-particle dimer amplifier; and 2, an LSPR amplifier based on the dimer structure is designed, The electric field amplification in the local area is realized, which has a positive effect on the enhancement detection of some weak signals. by wrapping the gain material outside the dimer, the absorption loss of the metal nanoparticles is overcome, and the local electric field around the dimer structure is greatly enhanced. The influence of the size of the nano-particle spacing d on the distribution of the local electric field is studied, and the near field enhancement of the LSPR amplifier is investigated under the condition of proper spacing, and its application prospect is discussed. The correlation results are calculated using the time-domain finite difference method (FDTD). By adjusting the spacing of the dimer nanoparticles, the specific signal enhancement was achieved. 3. The fluorescence emission principle of the molecules near the metal nanoparticles was used to study the interaction between the biomolecules. In addition to the commonly used parameters such as fluorescence intensity, we also use the parameters of quantum efficiency, fluorescence lifetime and so on to make relevant representation. The method can be applied to the binding action of protein, DNA and antibody-antigen. and the intermolecular binding effect can be more sensitively carried out. The emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles were calculated by the FDTD method, and the mechanism of energy exchange was discussed. by comparing the emission characteristics of the fluorescent molecules in the vicinity of the metal nanoparticles under different conditions, the spatial resolution characteristics of the fluorescent molecules are detected according to the quantum efficiency and the fluorescence lifetime, and the binding action between the biomolecules is studied.
【學位授予單位】：華東師范大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：TB383.1;O657.3
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