【摘要】：納米材料由于具有獨(dú)特的光電性能,受到眾多領(lǐng)域研究者的廣泛關(guān)注。近年來(lái),基于量子點(diǎn)和納米金構(gòu)建光學(xué)傳感器成為迅速發(fā)展的領(lǐng)域之一。本文研究構(gòu)筑了兩種檢測(cè)藥物及蛋白的光學(xué)傳感器。一種是基于目標(biāo)檢測(cè)物能夠有效地猝滅或增強(qiáng)巰基乙酸(TGA)修飾的Cd Te量子點(diǎn)的熒光,另一種是基于檢測(cè)物能夠引起納米金團(tuán)聚建立的比色分析方法。主要內(nèi)容如下:(1)分別基于替加環(huán)素對(duì)Cd Te量子點(diǎn)的熒光猝滅作用和使納米金團(tuán)聚的作用建立了兩種檢測(cè)替加環(huán)素的新方法,并進(jìn)行了比較。在優(yōu)化實(shí)驗(yàn)條件下,Cd Te量子點(diǎn)熒光猝滅的程度與替加環(huán)素濃度在0.11-35.56μg m L-1范圍內(nèi)呈現(xiàn)良好的線性關(guān)系,檢出限為3.93×10-2μg m L-1;納米金比色法測(cè)定替加環(huán)素的線性范圍為5.98×10-3-1.44×10-1μg m L-1和0.30-2.99μg m L-1,檢出限為1.07×10-3μg m L-1。將上述兩種方法用于實(shí)際樣品中替加環(huán)素的分析,回收率在94.60%-104.44%之間。(2)分別基于鹽酸林可霉素(LCM)對(duì)Cd Te量子點(diǎn)的熒光增敏作用和使納米金團(tuán)聚的性質(zhì)建立了兩種檢測(cè)LCM的新方法,并進(jìn)行了比較。在優(yōu)化條件下,在1-240μg m L-1范圍內(nèi),LCM濃度與量子點(diǎn)熒光增強(qiáng)程度呈良好的線性關(guān)系,檢出限為2.63×10-1μg m L-1;在1.00×10-3-2.00×10-2μg m L-1及3.00×10-2-1.20×10-1μg m L-1范圍時(shí),LCM濃度與納米金吸光度比值(A650/A519)分別呈現(xiàn)良好的線性關(guān)系,檢出限為1.27×10-4μg m L-1。將方法用于LCM片劑的分析,結(jié)果滿意。(3)分別基于依替米星(ETM)對(duì)Cd Te量子點(diǎn)的熒光增敏作用和使納米金團(tuán)聚的性質(zhì)建立了兩種檢測(cè)ETM的新方法,并進(jìn)行了比較。在優(yōu)化實(shí)驗(yàn)條件下,量子點(diǎn)熒光增敏程度與ETM濃度在5.55-266.40 ng m L-1范圍內(nèi)呈現(xiàn)良好的線性關(guān)系,檢出限為1.28 ng m L-1;納米金比色法測(cè)定ETM的線性范圍為1.00-20.00 ng m L-1及28.00-84.00 ng m L-1,檢出限為0.26 ng m L-1。兩種方法用于實(shí)際樣品中ETM的測(cè)定,回收率在95.0%-104.9%之間。(4)利用熒光光譜法、紫外光譜法、同步熒光及三維熒光光譜法研究了TGA-Cd Te量子點(diǎn)與FTO蛋白的相互作用。結(jié)果表明,TGA-Cd Te量子點(diǎn)能夠猝滅FTO蛋白的熒光,猝滅機(jī)理為靜態(tài)猝滅。通過(guò)計(jì)算得到了TGA-Cd Te量子點(diǎn)與FTO蛋白作用的結(jié)合常數(shù)及熱力學(xué)參數(shù)等。此外,基于FTO蛋白對(duì)TGA-Cd Te量子點(diǎn)的熒光增敏作用,建立了測(cè)定FTO蛋白的方法。線性范圍為5.52×10-9-6.62×10-7 mol L-1,檢出限為1.14×10-9 mol L-1。方法用于合成樣品中FTO蛋白的檢測(cè),結(jié)果滿意。
[Abstract]:Nanomaterials have attracted wide attention due to their unique optoelectronic properties. In recent years, the construction of optical sensors based on quantum dots and nanocrystalline gold has become one of the rapidly developing fields. In this paper, two optical sensors for the detection of drugs and proteins have been developed. One is based on the fact that the target detector can effectively quench or enhance the fluorescence of Cd Te quantum dots modified by mercaptoacetic acid (TGA), and the other is a colorimetric analysis method based on the method of colorimetric analysis, which can cause nano-gold agglomeration. The main contents are as follows: (1) based on the fluorescence quenching effect of tegacycline on Cd Te quantum dots and the effect of nano-gold agglomeration, two new methods for the detection of tegacycline were established and compared. Under the optimized experimental conditions, the fluorescence quenching of, Cd Te quantum dots showed a good linear relationship with the concentration of tegicycline in the range of 0.11-35.56 渭 g mL ~ (-1), and the detection limit was 3.93 脳 10 ~ (-2) 渭 g / mL ~ (-1). The linear range of tigicycline was 5.98 脳 10 ~ (-3) -1.44 脳 10 ~ (-1) 渭 g mL ~ (-1) and 0.30-2.99 渭 g / mL ~ (-1) with the detection limit of 1.07 脳 10 ~ (-3) 渭 g / m ~ (-1). The above two methods have been applied to the analysis of tegacycline in actual samples. The recoveries ranged from 94.60% to 104.44%. (2) based on the fluorescence sensitizing effect of lincomycin (LCM) on Cd Te quantum dots and the properties of nano-gold agglomeration, two new methods for the detection of LCM were established and compared. Under the optimized conditions, there was a good linear relationship between the concentration of LCM and the fluorescence enhancement of quantum dots in the range of 1-240 渭 g mL ~ (-1), and the detection limit was 2.63 脳 10 ~ (-1) 渭 g / m ~ (-1). In the range of 1.00 脳 10-3-2.00 脳 10-2 渭 g mL-1 and 3.00 脳 10-2-1.20 脳 10-1 渭 g mL-1, the linear relationship between the concentration of LCM and the gold absorbance ratio (A650/A519) was good. The detection limit is 1.27 脳 10 ~ (-4) 渭 g mL ~ (-1). The method has been applied to the analysis of LCM tablets with satisfactory results. (3) based on the fluorescence sensitizing effect of etemicin (ETM) to Cd Te quantum dots and the properties of nano-gold agglomeration, two new methods for the detection of ETM have been established and compared. Under the optimized experimental conditions, there was a good linear relationship between the fluorescence sensitivity of QDs and the concentration of ETM in the range of 5.55-266.40 ng mL ~ (-1), and the detection limit was 1.28 ng mL ~ (-1). The linear ranges for the determination of ETM by nano-gold colorimetry were 1.00-20.00 ng mL -1 and 28.00-84.00 ng mL -1, and the detection limit was 0.26 ng mL -1. The two methods have been applied to the determination of ETM in practical samples. The recoveries are in the range of 95.0-104.9%. (4) fluorescence spectrometry, UV spectrophotometry, The interaction between TGA-Cd Te quantum dots and FTO protein was studied by synchronous fluorescence and three dimensional fluorescence spectroscopy. The results show that TGA-Cd Te QDs can quench the fluorescence of FTO protein, and the quenching mechanism is static quenching. The binding constants and thermodynamic parameters of the interaction between TGA-Cd Te quantum dots and FTO protein were calculated. In addition, based on the fluorescence sensitizing effect of FTO protein on TGA-Cd Te quantum dots, a method for the determination of FTO protein was established. The linear range is 5.52 脳 10-9-6.62 脳 10-7 mol L-1 and the detection limit is 1.14 脳 10-9 mol L-1. The method has been applied to the determination of FTO protein in synthetic samples with satisfactory results.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;TP212

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