【摘要】：為了開(kāi)發(fā)具有熒光-磁共振雙模式成像功能的生物成像探針,有效結(jié)合磁共振和熒光2種成像技術(shù)的優(yōu)點(diǎn),本論文以具有良好單分散性和水溶性的超順磁性鈷摻雜的氧化鐵納米粒子Co_(0.9)Fe_(2.1)O_4為前驅(qū)體,將銪(Ⅲ)與4′-苯基-2,2′:6′,2″-聯(lián)三吡啶-6,6″-二甲胺四乙酸的熒光配合物(PTTA-Eu~(3+))及癌細(xì)胞靶向識(shí)別分子葉酸(FA)與納米粒子共價(jià)偶聯(lián),制備出了1種具有長(zhǎng)壽命熒光、超順磁性(橫向弛豫時(shí)間T2加權(quán)磁共振成像功能)及癌細(xì)胞靶向識(shí)別性能的多功能納米生物探針PTTA-Eu~(3+)-CoFeO-FA。這種納米生物探針不僅可發(fā)出銪(Ⅲ)配合物特有的長(zhǎng)壽命紅色熒光信號(hào),還具有良好的T2加權(quán)磁共振成像(MRI)造影劑功能(納米粒子水溶液的質(zhì)子橫向弛豫速率r_2達(dá)183.7L/(mmol·s),使其既可用于癌細(xì)胞的時(shí)間分辨熒光成像測(cè)定,也可用于活體動(dòng)物的磁共振成像檢測(cè)。利用所制備的新探針成功地實(shí)現(xiàn)了小鼠單核巨噬細(xì)胞白血病細(xì)胞Raw 264.7的時(shí)間分辨熒光成像和昆明鼠的活體T_2加權(quán)磁共振成像檢測(cè)。
[Abstract]:In order to develop a biological imaging probe with the function of fluorescence magnetic resonance dual-mode imaging, the advantages of magnetic resonance imaging and fluorescence imaging are effectively combined. In this paper, the superparamagnetic cobalt doped ferric oxide nanoparticles, Co_ (0.9) Fe_ (2.1) O _ 4, which have good monodispersity and water solubility, are used as precursors. A fluorescent complex (PTTA-Eu~ (3) of 2 "-bipyridine-6" -dimethylaminotetraacetic acid (PTTA-Eu~ (3) and its target recognition molecule folate (FA) were covalently coupled with nanoparticles. Super paramagnetism (transverse relaxation time T 2-weighted magnetic resonance imaging) and multifunctional nano-biological probe PTTA-Eu~ _ (3)-CoFeO-FA. for targeting recognition of cancer cells This kind of nanobiological probe can not only emit the long life red fluorescence signal of europium (鈪，

本文編號(hào)：2355686