【摘要】：本文詳細(xì)介紹了食品中抗生素的分類,來(lái)源以及危害。核酸適配體的功能及特點(diǎn),以及其應(yīng)用。復(fù)合納米材料的特點(diǎn)和在電化學(xué)適配體傳感器中的應(yīng)用。并對(duì)電化學(xué)適配體傳感器做了相關(guān)改進(jìn),用于檢測(cè)抗生素。本文對(duì)以下內(nèi)容做了研究:1、基于氧化石墨烯-聚苯胺(GO-PANI)和辣根過(guò)氧化物酶(HRP)的信號(hào)擴(kuò)增方案構(gòu)建了競(jìng)爭(zhēng)型適配體傳感器超靈敏檢測(cè)土霉素(OTC)。在此方案中,GO-PANI膜修飾在電極表面,金納米材料(AuNPs)通過(guò)恒電位溶出技術(shù)電沉積在GO-PANI膜表面。作為捕獲探針同時(shí)對(duì)于OTC具有強(qiáng)親和力和強(qiáng)選擇性的核酸適配體用HRP進(jìn)行標(biāo)記。該免疫傳感器對(duì)于OTC展示了良好的線性響應(yīng),其線性范圍是4.0×10-6 mg L-1-1.0mg L-1,最低檢測(cè)限達(dá)到2.3×10-6 mg L-1。該傳感器具有重復(fù)性好,穩(wěn)定性強(qiáng)的優(yōu)點(diǎn),并在實(shí)際樣品檢驗(yàn)中展示出低背景干擾和高靈敏度的優(yōu)點(diǎn)。2、利用了還原石墨烯-聚苯胺(GR-PANI)和聚酰胺樹(shù)枝形金納米復(fù)合材料(PAMAM-Au)在電極表面的自組裝。這兩種材料展現(xiàn)了優(yōu)秀的電子傳導(dǎo)能力和對(duì)生物分子的固定能力。在夾心結(jié)構(gòu)的特異性識(shí)別基礎(chǔ)上,檢測(cè)卡那霉素的信號(hào)是依靠辣根過(guò)氧化酶(HRP)催化下,對(duì)雙氧水(H2O2)電化學(xué)還原實(shí)現(xiàn)的。上述電化學(xué)傳感器的線性范圍是5×10-6-4×10-2μg m L-1,檢測(cè)限是4.6×10-6μg m L-1,展示了寬的線性范圍。另外,本傳感器具有經(jīng)濟(jì)性、高選擇性和特異性的優(yōu)勢(shì),在實(shí)際樣品和臨床診斷中可以作為檢測(cè)卡那霉素的一個(gè)有用工具。3、利用石墨烯-立體納米金修飾的玻碳電極(GR-3D Au/GCE)和核酸適配體以及辣根過(guò)氧化物酶共同標(biāo)記的金納米復(fù)合材料(Aptamer-AuNPs-HRP)作為放大策略,構(gòu)建了新的夾心型電化學(xué)傳感器。方案中,GR-3D Au通過(guò)快速的一步電化學(xué)還原得到,具有增強(qiáng)電極表面電子轉(zhuǎn)移速率和固定大量生物分子的優(yōu)點(diǎn)。Aptamer-AuNPs-HRP對(duì)土霉素(OTC)具有強(qiáng)識(shí)別作用和強(qiáng)特異性,一個(gè)金納米粒子表面可以固定多個(gè)aptamer和HRP,從而提高與OTC的識(shí)別作用力以及HRP的催化能力。基于上述信號(hào)放大的策略構(gòu)建的電化學(xué)適配體傳感器對(duì)OTC的線性檢測(cè)范圍是5×10-13-2×10-6 g m L-1,最低檢測(cè)限是4.98×10-13 g mL-1。另外,該適配體傳感器具有高選擇性,強(qiáng)特異性的優(yōu)點(diǎn),并在實(shí)際樣品檢測(cè)中有良好的回收率。
[Abstract]:The classification, source and harm of antibiotics in food were introduced in detail. The function and characteristics of aptamer and its application. Characteristics of composite nanomaterials and their applications in electrochemical adaptor sensors. The electrochemical aptamer sensor was improved to detect antibiotics. In this paper, the following contents were studied: 1. Based on the signal amplification scheme of graphene oxide Polyaniline (GO-PANI) and horseradish peroxidase (HRP), a competitive aptamer sensor for the detection of oxytetracycline (OTC).) was constructed. In this scheme, the GO-PANI film was modified on the electrode surface, and the gold nanocrystalline (AuNPs) was electrodeposited on the surface of the GO-PANI film by potentiostatic stripping technique. Nucleic acid aptamers with strong affinity and selectivity to OTC were labeled with HRP as a capture probe. The immunosensor showed a good linear response to OTC. The linear range was 4.0 脳 10-6 mg L-1-1.0mg L-1 and the minimum detection limit was 2.3 脳 10-6 mg L-1. The sensor has the advantages of good repeatability and high stability, and shows the advantages of low background interference and high sensitivity in the actual sample test. Self-assembly of reduced graphene Polyaniline (GR-PANI) and polyamide dendritic gold nanocomposites (PAMAM-Au) on the electrode surface was carried out. These two materials demonstrate excellent electronic conductivity and immobilization of biomolecules. On the basis of specific recognition of sandwich structure, the signal of kanamycin was determined by electrochemical reduction of hydrogen peroxide (H2O2) catalyzed by horseradish peroxidase (HRP). The linear range of the electrochemical sensor is 5 脳 10-6-4 脳 10-2 渭 g mL -1 and the detection limit is 4.6 脳 10-6 渭 g mL -1, which shows a wide linear range. In addition, the sensor has the advantages of economy, high selectivity and specificity. It can be used as a useful tool for the detection of kanamycin in actual samples and clinical diagnosis. The glassy carbon electrode (GR-3D Au/GCE) and the aptamer of nucleic acid and the gold nanocomposite material (Aptamer-AuNPs-HRP) labeled by horseradish peroxidase (horseradish peroxidase) were used as amplification strategy. A new sandwich electrochemical sensor was constructed. In the scheme, GR-3D Au was obtained by rapid one-step electrochemical reduction, which has the advantages of enhancing the electron transfer rate on the electrode surface and fixing a large number of biomolecules. Aptamer-AuNPs-HRP has strong recognition and specificity to oxytetracycline (OTC). Multiple aptamer and HRP, can be immobilized on the surface of gold nanoparticles to improve the recognition force with OTC and the catalytic ability of HRP. The linear detection range of electrochemical aptamer sensor based on the above signal amplification strategy for OTC is 5 脳 10-13 -2 脳 10-6 g mL -1 and the minimum detection limit is 4.98 脳 10 -13 g mL-1.. In addition, the aptamer sensor has the advantages of high selectivity and strong specificity, and has a good recovery rate in the actual sample detection.
【學(xué)位授予單位】：濟(jì)南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：O657.1;TB383.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 張愛(ài)春;周存;;DNA電化學(xué)生物傳感器的研究進(jìn)展[J];天津工業(yè)大學(xué)學(xué)報(bào);2010年03期

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本文編號(hào)：2323181