發(fā)布時(shí)間：2018-09-18 12:23

【摘要】：本文以聚乙二醇單甲醚(PEG)為原料,制備了聚乙二醇單甲醚納米金(PEG-納米金),并將聚乙二醇巰基功能化后制備了巰基化聚乙二醇單甲醚納米金(PEG-HS-納米金),然后將兩種納米金分別用于有毒小分子丙烯酰胺的檢測(cè)和吸附上。建立了一種基于聚乙二醇單甲醚穩(wěn)定的納米金可視化檢測(cè)丙烯酰胺(AA)的新方法,并用透射電子顯微鏡(TEM)、紫外可見(jiàn)光光度計(jì)(UV)、激光粒度分析儀(DLS)對(duì)聚乙二醇納米金進(jìn)行測(cè)試分析。結(jié)果表明:該聚乙二醇納米金核呈分散均勻的規(guī)則球體,且其平均粒徑為19nm。顆粒整體(納米金核和表面穩(wěn)定劑)粒徑集中分布在40 nm。濃度為0.004 mg/m L的丙烯酰胺能使聚乙二醇納米金發(fā)生團(tuán)聚,膠體由紅色變成紫色,濃度在0.004mg/m L-0.032 mg/m L之間時(shí),團(tuán)聚程度隨濃度的增加而增加,同時(shí)顆粒整體粒徑依次增大到170 nm、250 nm、600 nm。相同條件下,N,N-二甲基丙烯酰胺(DMAA)不能使聚乙二醇納米金發(fā)生團(tuán)聚。因此,可用該方法簡(jiǎn)單、快速的實(shí)現(xiàn)丙烯酰胺選擇性檢測(cè)。將巰基化聚乙二醇納米金用于丙烯酰胺吸附,研究了其吸附性能、吸附機(jī)理以及循環(huán)利用。氫核磁(1H-NMR)、高效液相色譜(HPLC)、紫外可見(jiàn)分光光譜(UV)、透射電子顯微電鏡(TEM)、激光粒度分析儀(DLS)等測(cè)試表明:該巰基化聚乙二醇納米金在有機(jī)溶劑和水溶液中均能吸附丙烯酰胺,但不吸附N,N-二甲基丙烯酰胺,這種吸附來(lái)源于丙烯酰胺的氫原子與巰基化聚乙二醇的氧原子之間的氫鍵。二氯甲烷中每個(gè)巰基化聚乙二醇納米金平均吸附25772個(gè)丙烯酰胺分子,水溶液中每個(gè)巰基化聚乙二醇納米金平均吸附22420個(gè)丙烯酰胺分子,比二氯甲烷中少,這可能是水溶液中水分子使丙烯酰胺與聚乙二醇之間的氫鍵作用減弱。另外,該巰基化聚乙二醇納米金前4次循環(huán)團(tuán)聚較少,循環(huán)5次之后,團(tuán)聚量超過(guò)50%,這可能是多次離心使納米金產(chǎn)出團(tuán)聚引起的。因此,該巰基化聚乙二醇納米金可能成為新型可循環(huán)利用的有毒小分子吸附劑。
[Abstract]:In this paper, polyethylene glycol monomethyl ether (PEG) was used as raw material. Polyethylene glycol monomethyl ether gold (PEG- nanocrystalline gold) was prepared and PEG-HS- nanocrystalline gold (PEG-HS- nanocrystalline gold) was prepared by functionalization of polyethylene glycol sulfhydryl group. Then the two kinds of gold nanoparticles were used as toxic small molecule propene respectively. Determination and adsorption of amides. A new method for visual detection of acrylamide (AA) based on polyethylene glycol monomethyl ether stabilized nanocrystalline gold was developed, and the (UV), laser particle size analyzer (DLS) was used to test and analyze polyethylene glycol nanocrystalline gold with transmission electron microscope (TEM),) ultraviolet visible photometer (TEM),). The results show that the nucleation of polyethylene glycol nanocrystalline is a uniform regular sphere with an average particle size of 19 nm. The particle size of the whole particle (nanocrystalline gold core and surface stabilizer) is concentrated at 40 nm.. Acrylamide with concentration of 0.004 mg/m / L could agglomerate polyethylene glycol nanocrystalline gold, and the colloid changed from red to purple. When the concentration was between 0.004mg/m L-0.032 mg/m L, the agglomeration degree increased with the increase of concentration, and the particle size increased to 170 nm,250 nm,600 nm. in turn. Under the same conditions, N- dimethylacrylamide (DMAA) could not agglomerate polyethylene glycol nanocrystalline gold. Therefore, the method can be simple and rapid to achieve acrylamide selective detection. The adsorption properties, adsorption mechanism and recycling of acrylamide were studied by using sulfhydrated polyethylene glycol nanocrystalline gold to adsorb acrylamide. The results of hydrogen NMR (1H-NMR), (HPLC), ultraviolet spectrophotometry (HPLC), (UV), transmission electron microscopy (UV), electron microscopy (TEM), laser particle size analyzer (DLS) and so on show that the sulfated polyethylene glycol nanocrystalline gold can adsorb acrylamide in both organic solvent and aqueous solution. However, N- dimethylacrylamide is not adsorbed, which is derived from the hydrogen bond between the hydrogen atom of acrylamide and the oxygen atom of mercapto polyethylene glycol. In dichloromethane, an average of 25772 acrylamide molecules were adsorbed by each mercapto polyethylene glycol nanocrystalline gold, and 22420 acrylamide molecules per mercapto polyethylene glycol nano-gold adsorbed in aqueous solution, less than that in dichloromethane. It is possible that water molecules in aqueous solution weaken the hydrogen bond between acrylamide and polyethylene glycol. In addition, the first four times of cyclic agglomeration of the mercapto PEG-nanocrystalline gold was less, and after five cycles, the agglomeration amount was more than 50, which may be caused by the agglomeration of nanocrystalline gold produced by centrifugation for many times. Therefore, the sulfhydryl polyethylene glycol nanocrystalline gold may be a new type of toxic small molecule adsorbent which can be recycled.
【學(xué)位授予單位】：西南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：TB383.1;O647.3

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